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H5 subtype avian influenza vaccine candidate strain rs‑156‑/170+/181‑ and its construction method and application

A construction method and avian influenza technology, which can be applied to biochemical equipment and methods, pharmaceutical formulations, viruses/phages, etc., and can solve problems such as poor cross-protection of branched viruses, inability of vaccines to provide protection, and changes in antigenicity and immunogenicity.

Active Publication Date: 2017-05-03
YANGZHOU UNIV
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Problems solved by technology

At present, the H5N1 subtype AIV prevalent in poultry in my country is mainly distributed in the 2.3.4 and 2.3.2 branches of the 2.3 branch and the 7.2 branch of the 7 branches, and the cross protection between viruses of different branches is poor
Moreover, these dominant epidemic viruses have further evolved into fourth-level branches, and further changes in antigenicity and immunogenicity have occurred. Existing vaccines cannot provide complete protection.

Method used

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  • H5 subtype avian influenza vaccine candidate strain rs‑156‑/170+/181‑ and its construction method and application
  • H5 subtype avian influenza vaccine candidate strain rs‑156‑/170+/181‑ and its construction method and application
  • H5 subtype avian influenza vaccine candidate strain rs‑156‑/170+/181‑ and its construction method and application

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Embodiment Construction

[0014] The biological materials involved in the present invention are as follows:

[0015] A / mallard / Huadong / S / 2005 (SY) strain (Tang Y, Wu P, Peng D, Wang X, Wan H, Zhang P, Long J, Zhang W, Li Y, Wang W, Zhang X, Liu X.Characterization of duck H5N1 influenza viruses with differing pathogenicity in mallard (Anas platyrhynchos) ducks. Avian Pathol. 2009 Dec; 38(6):457-67. Yanfang Li, Sujuan Chen, Xiaojian Zhang, Qiang Fu, Zhiye Zhang, Shaohua Shi, Yinbiao Zhu, Min Gu, Daxin Peng*, Xiufan Liu*. A 20-amino-acid deletion in the neuraminidase stalk and a five-amino-acid deletion in the NS1 protein both contribute to the pathogenicity of H5N1avian influenza. Plos one. 2014).

[0016] Concrete technical scheme of the present invention is as follows:

[0017] 1. Point Mutations and Rescue of Vaccine Strains

[0018] 1.1 Primer design

[0019] According to the A / mallard / Huadong / S / 2005 (SY) strain virus HA sequence (Genebank sequence number: EU195392.1, Tang, Y., Wu, P., Sun, Q., Pe...

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Abstract

The invention relates to a candidate strain of H5 subtype avian influenza vaccine and its construction method and application. The vaccine candidate strain is obtained by modifying the glycosylation site on the HA protein by screening the A / mallard / Huadong / S / 2005 (SY) strain as the parent, specifically the deletion of the 181-position glycosylation modification of the HA protein rS‑156‑ / 170+ / 181‑. The vaccine candidate strain has good serum cross-neutralization ability and can be used to prepare broad-spectrum H5 subtype AIV vaccine.

Description

technical field [0001] The invention relates to a method for modifying the glycosylation site of the H5 subtype avian influenza virus (AIV) HA protein by reverse genetic technology to construct a broad-spectrum H5 subtype AIV vaccine. The AIV mainly involved is distributed in 2.3.4 In branch, 2.3.2.1 branch and 7.2 branch. Background technique [0002] H5N1 subtype highly pathogenic avian influenza can cause high morbidity and mortality in poultry and is endemic in China, Vietnam, Indonesia, India, Bangladesh and Egypt (FAO, http: / / www.fao .org), causing huge economic losses to the poultry industry. Since the first report in 1997 that AIV can directly infect humans, there have been successive reports of H5N1, H9N2, H7N7 and H7N9 subtypes of AIV infecting humans. As of October 8, 2013, the World Health Organization (WHO) (http: / / www.who.int) reported that there were 641 cases of human infection with H5N1 subtype AIV in the world, of which 380 cases died, and the mortality r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00C12N15/09A61K39/145A61P31/16
Inventor 彭大新张晓剑陈素娟姜逸王宵刘秀梵
Owner YANGZHOU UNIV
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