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H5-subtype avian influenza vaccine candidate strain rS-156-/170+/181- as well as construction method and application thereof

A construction method and avian influenza technology, which can be applied to biochemical equipment and methods, pharmaceutical formulations, viruses/phages, etc., and can solve problems such as the inability of vaccines to provide protection, changes in antigenicity and immunogenicity, and poor cross-protection of branched viruses.

Active Publication Date: 2014-12-10
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the H5N1 subtype AIV prevalent in poultry in my country is mainly distributed in the 2.3.4 and 2.3.2 branches of the 2.3 branch and the 7.2 branch of the 7 branches, and the cross protection between viruses of different branches is poor
Moreover, these dominant epidemic viruses have further evolved into fourth-level branches, and further changes in antigenicity and immunogenicity have occurred. Existing vaccines cannot provide complete protection.

Method used

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  • H5-subtype avian influenza vaccine candidate strain rS-156-/170+/181- as well as construction method and application thereof
  • H5-subtype avian influenza vaccine candidate strain rS-156-/170+/181- as well as construction method and application thereof
  • H5-subtype avian influenza vaccine candidate strain rS-156-/170+/181- as well as construction method and application thereof

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Embodiment Construction

[0014] The biological materials involved in the present invention are as follows:

[0015] A / mallard / Huadong / S / 2005 (SY) strain (Tang Y, Wu P, Peng D, Wang X, Wan H, Zhang P, Long J, Zhang W, Li Y, Wang W, Zhang X, Liu X. Characterization of duck H5N1 influenza viruses with differing pathogenicity in mallard(Anas platyrhynchos) ducks.Avian Pathol.2009 Dec;38(6):457-67.Yanfang Li,Sujuan Chen,Xiaojian Zhang,Qiang Fu,Zhiye Zhang,Shaohua Shi, Yinbiao Zhu,Min Gu,Daxin Peng*,Xiufan Liu*.A 20-amino-acid deletion in the neuraminidase stalk and a five-amino-acid deletion in the NS1 protein both contribute to the pathogenicity of H5N1 avian influenza.Plos one. 2014).

[0016] The specific technical scheme of the present invention is as follows:

[0017] 1. Point mutation and the rescue of vaccine strains

[0018] 1.1 Primer design

[0019] According to the HA sequence of the A / mallard / Huadong / S / 2005 (SY) strain (Genebank sequence number: EU195392.1, Tang, Y., Wu, P., Sun, Q., Peng, D., Zhang, ...

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Abstract

The invention relates to an H5-subtype avian influenza vaccine (AIV) candidate strain as well as a construction method and an application thereof. The vaccine candidate strain is obtained by modifying glycosylation sites on HA protein by adopting an A / mallard / Huadong / S / 2005 (SY) strain as a female parent and screening, and particularly, the vaccine candidate strain is HA-protein-deleted rS-156- / 170+ / 181- modified by glycosylation at 181st position. The vaccine candidate strain has the advantage of good serum cross-neutralizing capacity and can be used for preparing broad-spectrum H5-subtype AIV vaccines.

Description

Technical field [0001] The present invention relates to a method for constructing a broad-spectrum H5 subtype AIV vaccine by modifying the glycosylation sites of the H5 subtype avian influenza virus (AIV) HA protein by reverse genetic technology, and the AIV mainly involved is distributed in 2.3.4 Branches, 2.3.2.1 branches and 7.2 branches. Background technique [0002] The H5N1 subtype highly pathogenic avian influenza can cause high morbidity and high mortality in poultry. It is endemic in China, Vietnam, Indonesia, India, Bangladesh and Egypt (FAO, http: / / www.fao .org), which has brought huge economic losses to the poultry industry. Since it was first reported in 1997 that AIV can directly infect humans, there have been reports of H5N1, H9N2, H7N7 and H7N9 subtypes AIV infecting humans. As of October 8, 2013, the World Health Organization (WHO) (http: / / www.who.int) reported that there have been 641 cases of human infection with H5N1 subtype AIV worldwide, of which 380 have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12N15/09A61K39/145A61P31/16
Inventor 彭大新张晓剑陈素娟姜逸王宵刘秀梵
Owner YANGZHOU UNIV
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