Tri-axial digital scanning light-sheet microscope based on axial ultrahigh-speed scanning

A digital scanning and microscopy technology, applied in the field of fluorescence microscopy, to achieve the effect of fast three-dimensional scanning

Active Publication Date: 2015-03-11
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2P3A-DSLM solves the problem of in vivo imaging of subcellular structures and dynamic processes of small model organisms

Method used

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  • Tri-axial digital scanning light-sheet microscope based on axial ultrahigh-speed scanning
  • Tri-axial digital scanning light-sheet microscope based on axial ultrahigh-speed scanning
  • Tri-axial digital scanning light-sheet microscope based on axial ultrahigh-speed scanning

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Experimental program
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Effect test

Embodiment 1

[0049] figure 2 It is a schematic diagram of the structure of 2P3A-DSLM. As shown in the figure, on the basis of the traditional light sheet microscope, a fast zoom device is set in the excitation light path for fast modulation of the focus position of light; a fast light intensity modulation device is set for fast modulation of light intensity; the fast zoom device is TAG . According to the actual situation, the reflective beam expander can expand the beam first to make the spot meet the TAG aperture requirements and reduce the chromatic aberration. Lens 1 and Lens 2 are used to conjugate the zoom plane of the TAG lens to the rear focal plane of the microscope objective. Lens 3 and lens 4 perform beam expansion for the second time, and conjugate the scanning angle of the scanner to the back focal plane of the excitation objective lens.

Embodiment 2

[0051] The fast zoom device is one of electronically controlled variable focus lenses and MEMSMIRROR.

Embodiment 3

[0053] The fast light intensity modulation device is a Pockels cell (Conoptics, 350-160LA).

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Abstract

The invention provides a tri-axial digital scanning light-sheet microscope based on axial ultrahigh-speed scanning, and mainly relates to the field of fluorescent microscopes. A beam light path rapid zooming device is arranged in an excitation light path and used for rapidly modulating the focal position of light, and a rapid light intensity modulation device is arranged and used for rapidly modulating light intensity so that the light-sheet microscope capable of increasing and adjusting scanning range without changing thickness of a light sheet is invented. Submicron level z-axis resolution, a visual field of the size of 170*170 mu m<2> in a deep tissue (greater than 500 mu m) and time resolution of being 1ms can be provided by the novel technology. A problem of long time of imaging of a small model organism subcellular structure and the living organism dynamic process is solved by 2P3A-DSLM.

Description

technical field [0001] The invention relates to a three-axis digital scanning light sheet microscope based on axial ultra-high-speed scanning, and mainly relates to the technical field of fluorescence microscopy. Background technique Background technique: [0002] The recent emergence of light-sheet fluorescence microscopy (LSFM) has revolutionized biological three-dimensional imaging. LSFM has the characteristics of high time resolution and small light damage, which makes it possible to observe small model organisms or tissues for a long time through fluorescence imaging. [0003] The light sheet microscope uses a layer of light beam to excite the fluorescent sample from the side of the sample, and detects the image through the photographic equipment, and its incident illumination light path and the photographic equipment receive the fluorescent light path perpendicular to each other. Classification of traditional light sheet microscopes: From the perspective of laser li...

Claims

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Application Information

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IPC IPC(8): G02B26/10G02F1/03G01N21/64
Inventor 陈良怡宗伟健孙育杰陈宣杨
Owner PEKING UNIV
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