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A method for producing rice-flavored liquor by liquid fermentation

A liquid fermentation and rice-flavored technology, which is applied in the field of wine making, can solve the problems of high labor intensity, low content of ester aroma substances, and development restrictions, and achieve the effects of reducing labor intensity, pure rice aroma, and improving quality

Active Publication Date: 2016-06-15
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] First, due to the saccharification of solid-state bacteria cultivation in the early stage, manual operation, high labor intensity, and the production process is greatly affected by natural factors such as climate and environment, resulting in unstable quality;
[0006] Two, although Xiaoqu production adopts inoculation (koji) cultivation, its manufacturing process is open, subject to the influence of natural environmental factors, in its Xiaoqu except containing a large amount of Rhizopus, yeast and other beneficial microorganisms for brewing wine, also entraining simultaneously. Harmful microorganisms in winemaking, which ultimately affect the quality of wine;
[0007] 3. Compared with Daqu liquor, the content of ester aroma substances is low, generally 1 / 4-1 / 2 of Daqu liquor, which makes rice-flavored liquor rank in the ranks of low-grade liquor in the liquor market for a long time, and its development is restricted;
[0008] 4. At present, an appropriate amount of active dry yeast (ADY) is used in the production of Xiaoqu wine. Although it can partially purify the fermentation system and help improve the yield of raw materials and stabilize wine quality, ordinary Saccharomyces cerevisiae will produce more during the growth and metabolism process. The higher alcohol content in the finished Xiaoqu wine is too high, generally up to about 50% of the total aroma components, it is not easy to digest and absorb after drinking, the stamina is strong, and it has intoxicating effect, that is, "top".

Method used

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  • A method for producing rice-flavored liquor by liquid fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the preparation of yeast culture fluid

[0029] (1) Preparation of rice saccharification liquid medium

[0030] ① Gelatinization and liquefaction: After crushing the rice, beat it with 4 times the amount of water, add 4 U of high-temperature-resistant α-amylase per gram of rice flour, and start stirring to mix well. In the state of stirring, heat to 90°C, keep warm and liquefy at this temperature for 15 minutes, seal the can, continue to heat to gauge pressure 0.15MPa, keep the pressure for 25 minutes, cool naturally until the gauge pressure is zero, open the can and cool to about 60°C .

[0031] ②Saccharification: Add 120U of glucoamylase per gram of raw material, stir for 15 minutes, then keep warm at 60°C for 2.5 hours, stir once every half hour during this period, and the starch saccharification rate should be about 60%.

[0032] ③ Medium preparation: Dilute the saccharification solution with water to 13Bx, adjust the pH to 5.0, then warm the prepare...

Embodiment 2

[0038] Embodiment 2: the preparation of lactic acid bacteria culture fluid

[0039] (1) Primary seed cultivation

[0040] The slant strains of Lactobacillus acidophilus (CICC6005) were inoculated into a 250mL Erlenmeyer flask filled with 50mL MRS liquid medium, cultured statically at 37°C for 30h, and the pH dropped to 4.0.

[0041] (2) Secondary seed cultivation

[0042] Dilute the rice saccharification liquid medium to 12Bx, add 1% barley root powder, sterilize at 110°C for 30 minutes, and obtain the lactic acid bacteria medium after cooling. Connect the first-grade seeds with 10% (V), culture statically at 37°C for 16 hours, and the lactic acid content reaches about 18g / L to obtain the lactic acid bacteria culture solution.

Embodiment 3

[0043] Example 3: Production of Rice-flavored Liquor by Liquid Fermentation of Xiaoqu Pure Grain

[0044] 1. Raw material crushing

[0045] 3.4kg of high-quality indica rice, crushed, of which more than 60% pass through a 20-mesh sieve.

[0046] 2. Liquefaction and gelatinization

[0047] Add 10L of water to the pulverized rice flour, stir well, add 4U of high-temperature-resistant α-amylase per gram of rice flour, stir well, heat to 85°C while stirring, and maintain at this temperature for 1h. Continue heating and gelatinize at 0.1MPa and 121°C for 60 minutes to obtain rice gelatinized liquid.

[0048] 3. Bacteria saccharification

[0049] Cool the rice gelatinized liquid to 40°C, add 50U / g of glucoamylase preparation, keep at 40°C, cool to 30°C after 30 minutes, add water to a total volume of 12L, add 0.8% raw material amount of Guilin Jiuqu pills, and connect lactic acid bacteria according to the volume ratio Culture medium 1.0%, yeast culture medium inoculum amount 2%,...

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Abstract

The invention discloses a method for producing rice-flavor liquor based on liquid fermentation, belonging to the technical field of liquor brewing. The method comprises the steps of crushing raw materials, liquefying gelatinization, culture saccharification, fermentation and distilling, wherein the step of culture saccharification is carried out under the state of tiny oxygen consumption and comprises cooling a saccharification liquid, adding glucoamylase, and subsequently adding xiaoqu yeast powder or rhizopus yeast, a lactobacillus culture solution and a microzyme culture solution. According to the method, mechanical production of xiaoqu liquor is achieved by cooking liquefaction of starchy grains and then liquid state culture saccharification, and therefore the defect of unstable quality caused by such factors as climate, environment during saccharification of culture bacteria is improved; simultaneously liquor brewing yeasts which are purely cultured and can produce high yield of esters and appropriately low yield of higher alcohols, lactobacillus and a part of commercial enzyme preparations are subjected to synergistic saccharification with conventional saccharification fermentation agents, thereby partially purifying the fermentation system, making up the deficiency of the conventional saccharification fermentation agents and greatly increasing the content of ester substances in rice-flavor liquor; and controlling the content of higher alcohols can remarkably improve the quality of the rice-flavor liquor.

Description

Technical field: [0001] The invention discloses a production method of rice-flavored liquor, which belongs to the technical field of brewing. Background technique: [0002] Rice-flavored liquor is represented by Guilin Sanhua Liquor, Quanzhou Xiangshan Liquor, and Guangdong Changle Burn. It is characterized by pure and elegant rice fragrance, soft and sweet entrance, refreshing and clean finish, and pleasant aftertaste. [0003] The general production method of rice-flavored liquor is as follows: rice is used as the raw material, and Xiaoqu is used as the saccharification and starter agent. In the early stage, the rice and Xiaoqu are mixed well, and then solid-state bacteria are cultivated for saccharification. In the later stage, water is added to turn the tank into a semi-liquid fermentation, and the mature mash is distilled into Xiaoqu wine. It belongs to semi-solid and semi-liquid fermentation liquor. [0004] The main problem that the preparation method of this rice-f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12G3/02C12R1/865
CPCC12G3/02C12H6/02
Inventor 肖冬光唐取来郭学武杜丽平张翠英陈叶福
Owner TIANJIN UNIV OF SCI & TECH
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