A microdevice and microscope system for studying egg cells

Abstract

The invention provides a micro device used for studying egg cells. The micro device used for studying egg cells comprises an egg cell micro-irrigation unit and a circulation refrigeration unit. The egg cell micro-irrigation unit comprises a first covering plate and a second covering plate which are used for fixing the egg cell micro-irrigation unit. The circulation refrigeration unit is tightly close to the first covering plate, a first film and a second film are sequentially arranged between the first covering plate and the second covering plate, and a micro-irrigation cavity is formed by a first curved groove in the first film and a second curved groove in the second film; the micro-irrigation cavity is provided with an inlet guide pipe and an outlet guide pipe, and temperature sensing equipment is arranged in the cavity; a fixing baffle which is used for fixing the positions of egg cells and permits flowing of a solution is arranged at the position, between the inlet guide pipe and the outlet guide pipe, in the cavity. The invention further provides a microscope system, after the micro device is connected with an irrigation microscope device, the formed microscope system can be used for measuring the size response of egg cells subjected to changes of the concentration at a specific temperature, and then the permeability parameter of the cell membrane is determined.

Description

technical field

[0001] The application belongs to the technical field of cryogenic biomedicine, and in particular relates to a microdevice and a microscope system for studying egg cells. Background technique

[0002] In recent years, the incidence of infertility has been increasing day by day, and traditional medicine and surgical treatment methods are far from meeting the needs of patients for fertility. Human assisted reproductive technology provides a new way for the cure of infertility. Due to the characteristics of assisted reproductive technology, cryopreservation of eggs is inevitably involved in the process of implementing this technology. The birth of oocyte cryopreservation technology is an important breakthrough in the field of biomedicine. By cryopreserving oocytes, it can help women who are infertile or have undergone several ovariectomies to obtain offspring. Therefore, cryopreservation of oocytes has broad prospects for development.

[0003] The typical cryo...

Examples

Embodiment

[0070] Structure of a microdevice used to study egg cells see Figure 1 to Figure 5 , wherein, the upper wall (the first cover) 11 and the lower wall (the second cover) 12 are silica glass; the first film 13 and the second film 14 are silica gel films, the thickness is 100 microns, 50 microns. The first film 13 is engraved with two short grooves 131 and 132 , and the second film 14 is engraved with a long groove (second groove) 141 . The width of the long groove and the two short grooves are both 200 μm, and the lengths are 44 mm, 30 mm, and 15 mm, respectively, and the outer edges are aligned. The first short groove 131 and the long groove 141 form a material chamber with a height of 150 microns, and the part between the two short grooves forms a baffle plate 19 with a height of 100 microns. The inlet conduit 15 is close to the edge of the material chamber, and the outlet conduit 16 is close to the other edge of the microperfusion chamber; both the inlet conduit 15 and the o...

technical field

[0001] The application belongs to the technical field of cryogenic biomedicine, and in particular relates to a microdevice and a microscope system for studying egg cells. Background technique

[0002] In recent years, the incidence of infertility has been increasing day by day, and traditional medicine and surgical treatment methods are far from meeting the needs of patients for fertility. Human assisted reproductive technology provides a new way for the cure of infertility. Due to the characteristics of assisted reproductive technology, cryopreservation of eggs is inevitably involved in the process of implementing this technology. The birth of oocyte cryopreservation technology is an important breakthrough in the field of biomedicine. By cryopreserving oocytes, it can help women who are infertile or have undergone several ovariectomies to obtain offspring. Therefore, cryopreservation of oocytes has broad prospects for development.

[0003] The typical cryo...

Examples

Embodiment

[0070] Structure of a microdevice used to study egg cells see Figure 1 to Figure 5 , wherein, the upper wall (the first cover) 11 and the lower wall (the second cover) 12 are silica glass; the first film 13 and the second film 14 are silica gel films, the thickness is 100 microns, 50 microns. The first film 13 is engraved with two short grooves 131 and 132 , and the second film 14 is engraved with a long groove (second groove) 141 . The width of the long groove and the two short grooves are both 200 μm, and the lengths are 44 mm, 30 mm, and 15 mm, respectively, and the outer edges are aligned. The first short groove 131 and the long groove 141 form a material chamber with a height of 150 microns, and the part between the two short grooves forms a baffle plate 19 with a height of 100 microns. The inlet conduit 15 is close to the edge of the material chamber, and the outlet conduit 16 is close to the other edge of the microperfusion chamber; both the inlet conduit 15 and the o...

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