Deoxycholate hydrogen sulfide lactose agar medium and use thereof

A technology of bile sulfur milk agar and culture medium, which is applied in the field of microbiology to achieve the effects of simple formula, rapid bacterial growth and low mutation rate

Inactive Publication Date: 2015-05-27
刘汉斌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Except typhoid bacillus, paratyphoid A and paratyphoid bacillus causing human diseases, most of them can only cause animal diseases such as livestock, rodents and poultry, but sometimes they can also contaminate human food and cause food poisoning

Method used

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Examples

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Effect test

Embodiment 1

[0022] A bilesulfuric milk agar medium, the components of the medium include 20.1g / L GN enrichment liquid, 8.0g / L lactose, 2.8g / L sodium thiosulfate, 1.5g / L sodium citrate, 1.5 g / L ferric ammonium citrate, 0.023g / L neutral red, 16.5g / L agar, pH value is 7.1-7.3.

[0023] The GN enrichment solution contains 20.0g tryptone, 2.0g glucose, 1.5g dipotassium hydrogen phosphate, 3g mannitol, 5.8g sodium citrate, and 0.33g sodium deoxycholate per liter of GN enrichment solution. Sodium chloride 5.0g, dipotassium hydrogen phosphate 2.0g.

[0024] Peptone and glucose provide carbon source, nitrogen source, vitamins and minerals; lactose is a fermentable sugar; sodium deoxycholate and sodium citrate inhibit gram-positive bacteria and most coliforms and proteus, but Does not affect the growth of Salmonella; sodium thiosulfate and ferric ammonium citrate are used to detect the production of hydrogen sulfide, so that the center of the colony is black; neutral red is a pH indicator, and t...

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Abstract

The invention belongs to the field of microbes and relates to a deoxycholate hydrogen sulfide lactose agar medium. The deoxycholate hydrogen sulfide lactose agar medium comprises 20.1g / L of GN enrichment broth, 8.0g / L of lactose, 2.8g / L of sodium hyposulfite, 1.5g / L of sodium citrate, 1.5g / L of ammonium ferric citrate, 0.023g / L of neutral red and 16.5g / L of agar. The deoxycholate hydrogen sulfide lactose agar medium has a pH value of 7.1-7.3. Each liter of the GN enrichment broth comprises 20.0g of tryptone, 2.0g of glucose, 1.5g of dipotassium hydrogen phosphate, 3g of mannitol, 5.8g of sodium citrate, 0.33g of deoxysodium cholate, 5.0g of sodium chloride and 2.0g of dipotassium hydrogen phosphate. The deoxycholate hydrogen sulfide lactose agar medium has a simple formula, can be prepared conveniently, has a low cost, is conducive to popularization application, promotes bacterium fast growth, reduces a variation rate and is convenient for observation and analysis.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a gallic acid milk agar medium and an application thereof. Background technique [0002] The causative agent of salmonellosis. Enterobacteriaceae, Gram-negative enterobacteriaceae. Nearly a thousand species (or strains) have been discovered. According to its antigenic composition, Salmonella can be divided into basic bacterial groups such as A, B, C, D, and E. Among them, those related to human diseases mainly include A. paratyphi in Group A, B. paratyphi and Bacillus typhimurium in group B, C. paratyphi and Bacillus choleraesuis in group C, typhoid bacillus and Enteritidis bacteria in group D, etc. In addition to typhoid bacillus, paratyphoid A and paratyphoid bacillus causing human diseases, most of them can only cause animal diseases such as livestock, rodents and poultry, but sometimes they can also contaminate human food and cause food poisoning. Salmonella ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/42
CPCC12N1/20
Inventor 刘汉斌
Owner 刘汉斌
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