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A kind of eucalyptus leaf extract with anticancer effect and its preparation method and application

A technology of tussah leaves and extracts is applied in the field of preparing medicines that have an inhibitory effect on tumor cell growth, can solve problems such as no reports in experimental research, and achieve the effects of simple process flow, solvent residue avoidance and low cost.

Active Publication Date: 2017-11-10
辽宁省农业科学院大连生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no report on the related experimental research on the preparation of eucalyptus leaf extract with anti-cancer effect using eucalyptus leaves as raw materials

Method used

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  • A kind of eucalyptus leaf extract with anticancer effect and its preparation method and application
  • A kind of eucalyptus leaf extract with anticancer effect and its preparation method and application
  • A kind of eucalyptus leaf extract with anticancer effect and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Using Quercus japonica oak leaves as raw material, the preparation method of the oak leaf extract comprises the following steps:

[0040] ①Wash the leaves with water, dry them in the shade at 25°C, crush them to 40 mesh, and extract them with deionized water at a material-to-liquid ratio of 1g / 15mL at 20°C for 12 hours each time. Use 4 layers of gauze to filter and then soak the residue Extraction, total leaching 3 times, combined extracts; centrifuged the combined extracts at 3000rpm for 10min to obtain extracts; evaporated and concentrated the extracts at 65°C to 5% of the volume of the extracts to obtain concentrates;

[0041] ② Dilute the concentrated solution obtained in step ① to 10 times the volume of the concentrated solution with deionized water, filter it at 0.05-0.08MPa in an ultrafiltration membrane device with a molecular weight cut-off of 5000Dr, and collect the extract with a molecular weight below 5000Dr.

[0042] ③ Freeze-drying the extract obtained in ...

Embodiment 2

[0044]Using oak leaves of Quercus liaotungensis as raw material, the preparation method of the extract of oak leaves is the same as in Example 1, the only difference is that step ① of this example is: wash the leaves of oak leaves with water, dry them in the shade at 25°C, and crush them to 40 mesh With 65% ethanol aqueous solution with a volume fraction of 1g / 15mL, extract at 20°C for 12 hours each time, use 4 layers of gauze to filter, then extract the residue, and extract 3 times in total. Extraction solution; the combined extraction solution was centrifuged at 3000rpm for 10min to obtain the extraction solution; the extraction solution was evaporated and concentrated at 60°C to 5% of the volume of the extraction solution to obtain the first concentrated solution, which was added to the first concentrated solution Add 10 times the volume of deionized water, evaporate and concentrate at 60°C to the volume of the first concentrated solution to obtain a concentrated solution. ...

Embodiment 3

[0046] Using oak leaves of Quercus liaotungensis as raw material, the preparation method of the extract of oak leaves is the same as in Example 1, the only difference is that step ① of this example is: wash the leaves of oak leaves with water, dry them in the shade at 25°C, and crush them to 40 mesh With 75% ethanol aqueous solution with a volume fraction of 1g / 15mL, extract at 20°C, each extraction time is 12h, use 4 layers of gauze to filter and then extract the residue, a total of 3 extractions, combined Extraction solution; the combined extraction solution was centrifuged at 3000rpm for 10min to obtain the extraction solution; the extraction solution was evaporated and concentrated to 5% of the volume of the extraction solution at 60°C to obtain the first concentrated solution, which was added to the first concentrated solution Add 10 times the volume of deionized water, evaporate and concentrate at 60°C to the volume of the first concentrated solution to obtain a concentra...

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Abstract

The invention discloses an eucalyptus leaf extract with anticancer effect and its preparation method and application. The preparation method comprises the following steps: ① extract the eucalyptus leaves with an extraction agent to obtain an extract, evaporate and concentrate the extract Extract the solution to obtain a concentrated solution; ② After diluting the concentrated solution obtained in step ① with water, ultra-filter to obtain an oak leaf extract with a molecular weight ≤ 5000Dr; ③ Freeze-dry the extract obtained in step ② to obtain a freeze-dried powder, that is, an oak leaf extract. The eucalyptus leaf extract with anticancer effect of the invention can be used to prepare medicines with inhibitory effect on tumor cell growth. The experimental research on the preparation of the anti-cancer effect of the oak leaf extract described in the present invention is the first, which fills the research gap in this field. The prepared oak leaf extract can inhibit the growth of various tumor cells in vivo and in vitro, and has strong anti-tumor effect. cancer effect. The invention has simple technological process, less equipment investment, easy industrial production, and no pollution in the preparation technological process.

Description

technical field [0001] The present invention relates to an extract of eucalyptus leaves, in particular to an extract of eucalyptus leaves with an anticancer effect, a preparation method of the eucalyptus leaf extract and its use in the preparation of medicines with inhibitory effect on tumor cell growth . Background technique [0002] Oak tree (Querus mongolica Fisch) is a deciduous tree of the family Fagaceae, the genus Quercus. There are species of Quercus mongolica, Quercus liaotungensis, Quercus mongolica, and oak tree. The leaf margins are serrated and rarely entire. The catkins of the male flowers are drooping, and the female flowers are solitary in the involucre. The nut is solitary, the inner wall of the pericarp is glabrous, and the rudimentary ovule is located outside the base of the seed. The trunk is peculiar and vigorous, the shape of the tree is beautiful and varied, the branches and leaves are luxuriant, it is resistant to pruning, and it is easy to shape. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A23L33/00A61P35/00
Inventor 王林美岳冬梅夏兴宏李树英
Owner 辽宁省农业科学院大连生物技术研究所
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