A kind of crude extract of Paenibacillus bacteriocin and its preparation method and application

Abstract

The invention discloses a paenibacillus sp. bacteriocin crude extract as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) inoculating paenibacillus sp. into a culture medium for culturing the paenibacillus sp., shocking and carrying out fermentation cultivation to obtain fermentation liquor, and centrifuging the obtained fermentation liquor so as to take liquid supernatant; and (2) adding ammonium sulfate to the obtained liquid supernatant, centrifuging and collecting precipitates which are separated out when the saturation of the ammonium sulfate is 30-70%, dissolving the obtained precipitates and then dialyzing, and carrying out freeze drying to obtain the paenibacillus sp. bacteriocin crude extract. Raw materials for preparing the paenibacillus sp. bacteriocin crude extract are bran, wide in source and low in cost, and a fermentative strain is a paenibacillus sp. single strain; and the technology facilitates the standardization of product quality and the cost control of industrialized mass production. The paenibacillus sp. bacteriocin crude extract can be directly applied to food production, thus achieving the purposes of inhibiting the proliferation of putrefying bacteria and prolonging the shelf period of food and reducing the application cost.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a crude extract of Paenibacillus bacteriocin and its preparation method and application. Background technique

[0002] Bacteriocins are substances with antibacterial activity produced by certain bacteria, and their main components are polypeptides, proteins or protein complexes, which were first proposed by Jacob in 1953. In recent years, the negative effects brought about by the popularization and even abuse of broad-spectrum antibiotics have become increasingly prominent, and the research on new antibiotic substitutes is imminent. Bacteriocins, as protein substances, are highly safe because they can be degraded by proteases, so they have received more and more attention. Although the currently developed bacteriocin (Nisin) has a very high antiseptic and antibacterial application value, due to the characteristics of Nisin itself (stable properties under low pH condition...

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