A kind of water-insoluble exopolysaccharide of leuconostoc enterococcus and preparation method thereof

A Leuconostoc enterococci, water-insoluble technology, applied in the biological field, can solve the problems of cumbersome preparation methods, single type of glycosidic bond in the connection structure, narrow source of water-insoluble polysaccharides, etc., and achieve high food safety and broad application prospects.

Active Publication Date: 2018-01-16
BRIGHT DAIRY & FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved by the present invention is that the current source of water-insoluble polysaccharides is narrow, the connection structure of the branch chain and the main chain has a single type of glycosidic bond, the preparation method is cumbersome, and the strains used for the preparation are harmful bacteria. The present invention provides a Water-insoluble exopolysaccharide of leuconostoc enterococcus and preparation method thereof

Method used

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  • A kind of water-insoluble exopolysaccharide of leuconostoc enterococcus and preparation method thereof
  • A kind of water-insoluble exopolysaccharide of leuconostoc enterococcus and preparation method thereof
  • A kind of water-insoluble exopolysaccharide of leuconostoc enterococcus and preparation method thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0032]Example 1 Preparation of the water-insoluble exopolysaccharide of Leuconostoc enterococci

[0033] 1. Materials and methods

[0034] Preparation of seeds (fermented strains): dissolve the lyophilized powder of Leuconostoc mesenteroides BD3749 with a small amount of sterile distilled water, and use an inoculation loop to get a ring and streak it on the M17 solid medium containing 5% sucrose ( Purchased from OXOID, UK), 30°C aerobic culture for 48h, took out a single colony with an inoculation loop and put it into 10mL M17 liquid medium containing 5% sucrose (purchased from OXOID, UK), and used a vortex shaker to homogenize the colony Disperse in the liquid medium, 30°C, 180rpm shaking culture for 24h, take out, inoculate with 2% (v / v) inoculum size in M17 liquid medium containing 5% sucrose (purchased from OXOID, UK), 30°C, 180rpm shaking After culturing for 24 hours, the culture was centrifuged at 15,000rpm for 10 minutes, the supernatant was discarded, the bacteria wer...

Embodiment 2

[0040] Example 2 Preparation of the water-insoluble exopolysaccharide of Leuconostoc enteroenteritis

[0041] 1. Materials and methods

[0042] The preparation of seed (fermentation strain): with embodiment 1.

[0043] Synthetic medium (peptone 1%, yeast extract 0.5%, K 2 HPO 4 0.5%, CaCl 2 0.034%, sucrose 30%, described percentage is the preparation of mass percent): with peptone 10g, yeast extract 5g, sucrose 300g, K 2 HPO 4 20g and CaCl 2 0.034g mixed with 1L distilled water, fully dissolved, with NaHCO 3 Adjust the pH to 8 and sterilize to obtain a synthetic medium with the required sucrose concentration.

[0044] 2. Preparation of water-insoluble exopolysaccharides from Leuconostoc enterolis

[0045] Leuconostoc enterococcus seed solution was inoculated in a 5% (v / v) inoculation amount into a synthetic medium with a sucrose mass percentage of 30% and a pH of 8, 15°C, 300rpm shaking fermentation culture for 24 hours to obtain a fermentation broth, After the fermen...

Embodiment 3

[0048] Example 3 Preparation of the water-insoluble exopolysaccharide of Leuconostoc enteroenteritis

[0049] 1. Materials and methods

[0050] The preparation of seed (fermentation strain): with embodiment 1.

[0051] Synthetic medium (peptone 1%, yeast extract 0.5%, K 2 HPO 4 0.5%, CaCl 2 0.034%, sucrose 5%, the percentage is the preparation of mass percent): with peptone 10g, yeast extract 5g, sucrose 50g, K 2 HPO 4 20g and CaCl 2 Mix 0.034g with 1L of distilled water, fully dissolve, adjust the pH to 5 with NaOH, and sterilize to obtain a synthetic medium with the required sucrose concentration.

[0052] 2. Preparation of water-insoluble exopolysaccharides from Leuconostoc enterolis

[0053] Leuconostoc enterococcus seed solution was inoculated in a 1% (v / v) inoculation amount into a synthetic medium with 5% sucrose mass percentage and a pH of 5, 37°C, 100rpm shaking fermentation culture for 96 hours to obtain a fermentation broth, After lyophilization of the ferme...

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Abstract

The invention discloses water-insoluble exopolysaccharide of leuconostoc mesenteroides and a preparation method thereof. The water-insoluble exopolysaccharide of leuconostoc mesenteroides is glucan of which the main chain is formed through connection of alpha-(1,6) glycosidic bonds and the branched chain is formed through connection of alpha-(1,3) and alpha-(1,4) glycosidic bonds, wherein the molar ratio of the glycosidic bonds alpha-(1,6), alpha-(1,3) and alpha-(1,4) is 1:0.2-0.3:0.3-0.4. Compared with other water-insoluble polysaccharide generated by bacterial strains coming from other sources, the water-insoluble exopolysaccharide possesses a special structure, and is a water-insoluble glucan simultaneously possessing alpha-(1,3) and alpha-(1,4) glycosidic bonds. The source of the water-insoluble exopolysaccharide is safe and reliable, the preparation method is relatively simple, is relatively suitable for industrialized production and application, and possesses extremely extensive application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a water-insoluble exopolysaccharide of Leuconostoc enterococcus and a preparation method thereof. Background technique [0002] Water-insoluble glucan (Insoluble glucan, IG) is a kind of sugar polymer that is insoluble in aqueous solution and polymerized with glucose as a single component. It has been reported in the metabolites of , fungi and bacteria, and there are many kinds. At present, there are three kinds of IGs that are reported more: dextran, curdlan and cellulose. From the perspective of structural characteristics, the branches of these IGs that have been reported are only composed of α-(1,2), α-(1,3) or α-(1 ,4) One of the glycosidic bonds connects the branched chain to the main chain, and rarely involves two or more glycosidic bonded branch structures. Therefore, the screening and discovery of new configurations of IG not only adds new members to the IG fami...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02C12P19/08C12R1/01
CPCC08B37/0009C12P19/08
Inventor 鄢明辉吴正均韩瑨徐晓芬冯华峰
Owner BRIGHT DAIRY & FOOD CO LTD
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