A kind of preparation method of the water-insoluble exopolysaccharide of enterococci

A water-insoluble technology of Leuconostoc enterococcus, applied in the biological field, can solve the problems of high cost, food safety risk, cumbersome medium preparation, etc., and achieve the effect of broad application prospects and high food safety

Active Publication Date: 2020-11-27
BRIGHT DAIRY & FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems that the current preparation of water-insoluble dextran uses a synthetic medium with various components and complex formulas, the cost is relatively high, the preparation of the medium is cumbersome, and there are food safety risks, etc. Preparation method of bacterial water-insoluble exopolysaccharide

Method used

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  • A kind of preparation method of the water-insoluble exopolysaccharide of enterococci
  • A kind of preparation method of the water-insoluble exopolysaccharide of enterococci
  • A kind of preparation method of the water-insoluble exopolysaccharide of enterococci

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Preparation and yield of Leuconostoc enteroconostoc water-insoluble exopolysaccharide

[0034] 1. Materials and methods

[0035] Preparation of seeds (fermented strains): dissolve the lyophilized powder of Leuconostoc mesenteroides (the preservation number of the Leuconostoc mesenteroides is CGMCC No.10064) with a small amount of sterile distilled water, and use an inoculation loop Take a ring and streak it on M17 solid medium containing 5% sucrose (purchased from OXOID Co., UK), culture it aerobically at 30°C for 48 hours, pick a single colony with an inoculation loop and put it into 10 mL of M17 liquid containing 5% sucrose Culture medium (purchased from OXOID Co., UK), using a vortex shaker to evenly disperse the colonies in the liquid medium, 30°C, 180rpm shaking culture for 24h, take it out, and inoculate it with 2% (v / v) inoculum in 5 % sucrose M17 liquid medium (purchased from OXOID Co., UK), 30°C, 180rpm shaking culture for 24h, the culture was centri...

Embodiment 2

[0041] Example 2 Preparation and yield of Leuconostoc enterococci water-insoluble exopolysaccharide

[0042] 1. Materials and methods

[0043] The preparation of seed (fermentation strain): with embodiment 1.

[0044] Preparation of tomato juice sucrose medium: ripe tomatoes were cleaned, peeled, squeezed by a juicer, filtered through 120-mesh gauze to get the juice, boiled for 10 minutes, centrifuged at 4000g for 12 minutes to get the supernatant, added 30% sucrose by mass, heated to dissolve and cooled to Room temperature, with NaHCO 3 Adjust the pH to 8, sterilize at 135°C for 10 minutes, and cool to room temperature to obtain a sterile tomato juice sucrose medium.

[0045] 2. Preparation of water-insoluble exopolysaccharides from Leuconostoc enterolis

[0046] Inoculate the obtained Leuconostoc enterococcus seed liquid into a sucrose mass percentage of 30% and a pH of 8 in the tomato juice sucrose medium at an inoculum size of 5% (v / v), 15°C, 300rpm shaking fermentation...

Embodiment 3

[0049] Example 3 Preparation and Yield of Leuconostoc Enteritidis Water-Insoluble Exopolysaccharide

[0050] 1. Materials and methods

[0051] The preparation of seed (fermentation strain): with embodiment 1.

[0052] Preparation of tomato juice sucrose medium: ripe tomatoes were cleaned, peeled, squeezed by a juicer, filtered through 80-mesh gauze to get the juice, boiled for 1 min, centrifuged at 12000g for 8 min to get the supernatant, added with a mass percentage of 5% sucrose, heated to dissolve and cooled to At room temperature, adjust the pH to 5 with NaOH, sterilize at 110°C for 30 minutes, and cool to room temperature to obtain a sterile tomato juice sucrose medium.

[0053] 2. Preparation of water-insoluble exopolysaccharides from Leuconostoc enterolis

[0054] Inoculate the obtained Leuconostoc enterococcus seed liquid into the tomato juice sucrose medium with 5% sucrose mass percentage and pH 5 according to the inoculum size of 1% (v / v), 37°C, 100rpm shaking ferm...

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Abstract

The invention discloses a preparing method of water insolubility exopolysaccharides of leuconostoc mesenteroides. The method comprises following steps: inoculating the leuconostoc mesenteroides BD 3749 into a tomato juice saccharose culture medium, and fermenting to culture to obtain a fermented solution; (2) freezing and drying the fermented solution in step (1), then extracting powder with alkali liquor, taking supernatant, adjusting pH of the supernatant to 7, taking sediment and freezing and drying to obtain the water insolubility exopolysaccharides. According to the preparing method, the tomato saccharose culture medium takes the tomato juice saccharose as a main raw material and is wide in source, low in cost and natural and safe, food safety is improved while a material cost is reduced, fermented strain is single leuconostoc mesenteroides, and above combination is favorable for standardization of product quality and cost control of industrial large scale production, and has a very broad application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of water-insoluble exopolysaccharide of Leuconostoc enterococcus. Background technique [0002] Water-insoluble glucan (Insoluble glucan, IG) is a kind of sugar polymer that is insoluble in water and polymerized with glucose as a single component. This kind of carbohydrate with special physical and chemical properties not only has a wide range of sources, but also has It has been reported in the metabolites of , fungi and bacteria, and there are many kinds. At present, there are three kinds of IGs that are reported more: dextran, curdlan and cellulose. From the perspective of structural characteristics, the branches of these IGs that have been reported are only composed of α-(1,2), α-(1,3) or α-(1 ,4) One of the glycosidic bonds connects the branched chain to the main chain, and rarely involves two or more glycosidic bonded branch structures. Theref...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12R1/01
CPCC12P19/04
Inventor 吴正钧韩瑨徐晓芬高彩霞吴江
Owner BRIGHT DAIRY & FOOD CO LTD
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