A kind of Paenibacillus bacteriocin extract and its preparation method and application

A technology of Paenibacillus and Bacillus, applied in the field of Paenibacillus bacteriocin extract and its preparation, can solve the problems of insufficient sources, high preparation costs, and poor stability of bacteriocins, and achieve wide sources, low cost, and excellent application value Effect
CN104762353BActive Publication Date: 2018-04-27BRIGHT DAIRY & FOOD CO LTD
2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BRIGHT DAIRY & FOOD CO LTD
Publication Date
2018-04-27

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a bacillus genus bacteriocin extract, a preparation method and application thereof. Wherein said preparation method comprises the following steps: (1) inoculating Paenibacillus sp. in a tomato juice medium and shaking culture to obtain a fermented liquid; (2) centrifuging the fermented liquid obtained in step (1) to obtain the supernatant, The low molecular fraction was obtained by ultrafiltration and freeze-dried. At present, the preparation of bacteriocin extract relies on chemical synthesis medium, and the research of using tomato juice to prepare bacteriocin extract has not been reported. The present invention applies tomato juice medium as a natural fermentation medium to bacteriocin for the first time. The preparation of the extract, so its products have higher food safety. The raw material of the Paenibacillus bacteriocin extract is tomato, which has wide sources and low cost, and the fermentation strain is a single strain of Paenibacillus. The above-mentioned process is beneficial to standardization of product quality and cost control of large-scale industrial production.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a Paenibacillus bacteriocin extract and a preparation method and application thereof. Background technique

[0002] Bacteriocins are substances with antibacterial activity produced by certain bacteria, and their main components are polypeptides, proteins or protein complexes, which were first proposed by Jacob in 1953. In recent years, the negative effects brought about by the popularization and even abuse of broad-spectrum antibiotics have become increasingly prominent, and the research on new antibiotic substitutes is imminent. Bacteriocins, as protein substances, are highly safe because they can be degraded by proteases, so they have received more and more attention. Although the currently developed bacteriocin (Nisin) has a very high antiseptic and antibacterial application value, due to the characteristics of Nisin itself (stable properties under low pH conditions, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More