Oriental lily preservation method
A preservation method, lily technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of in vitro preservation of oriental lily and germplasm resources, so as to improve the efficiency of in vitro preservation and reduce the cost of preservation , The effect of improving the survival rate
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[0025] The invention discloses a method for preserving oriental lily, which comprises the following steps:
[0026] A. Preparation of medium: including basic medium and in vitro storage, the components of the medium at each stage and the weight of each component in each liter of medium are:
[0027] (1) Basic medium: Among them,
[0028] The induction medium is MS medium with white sugar 40 to 45g / L, agar powder 3 g / L, pH5.7;
[0029] Proliferation medium is white sugar 45 to 49g / L, agar powder 6 g / L, MS medium with pH5.7;
[0030] The culture medium for retirement storage and bulb expansion in test tubes is MS medium with 80 g / L sucrose, 8 g / L agar powder, and pH 5.8;
[0031] (2) Induction medium 1: MS+6-BA 1.5 g / L+IBA0.3 m g / L+AC2 m g / L;
[0032] (3) Induction medium 1: MS+6-BA 1.5 g / L+IBA0.3 m g / L+AC2 m g / L;
[0033] (4) Proliferation medium: 5MS+6-BA0.6 mg / L++IBA0.3 mg / L;
[0034] (5) Synchronous culture medium for in vitro preservation and tube bulb expansion: 1MS+N...
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