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A preservation method, lily technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of in vitro preservation of oriental lily and germplasm resources, so as to improve the efficiency of in vitro preservation and reduce the cost of preservation , The effect of improving the survival rate
Inactive Publication Date: 2015-09-23
LIUZHOU HELIAN AGRI
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[0003] Oriental lily is a perennial ornamental plant, which is mainly used for cut flower cultivation. In recent years, the annual growth of lily cut flower production in my country has reached more than 15, but it is currently impossible to achieve in vitro preservation of oriental lily germplasm resources.
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[0025] The invention discloses a method for preserving oriental lily, which comprises the following steps:
[0026] A. Preparation of medium: including basic medium and in vitro storage, the components of the medium at each stage and the weight of each component in each liter of medium are:
[0027] (1) Basic medium: Among them,
[0028] The induction medium is MS medium with white sugar 40 to 45g / L, agar powder 3 g / L, pH5.7;
[0029] Proliferation medium is white sugar 45 to 49g / L, agar powder 6 g / L, MS medium with pH5.7;
[0030] The culture medium for retirement storage and bulb expansion in test tubes is MS medium with 80 g / L sucrose, 8 g / L agar powder, and pH 5.8;
[0031] (2) Induction medium 1: MS+6-BA 1.5 g / L+IBA0.3 m g / L+AC2 m g / L;
[0032] (3) Induction medium 1: MS+6-BA 1.5 g / L+IBA0.3 m g / L+AC2 m g / L;
[0034] (5) Synchronous culture medium for in vitro preservation and tube bulb expansion: 1MS+N...
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Abstract
The invention provides an oriental lily preservation method including the following steps that plants which grow normally and luxuriantly and is not subjected to genetic variation after ISSR molecular marker detection are selected, and qualified oriental lily seedballs are cultivated; a field planting substrate is prepared by mixing peat and perlite according to the volume ratio of 8:5. The oriental lily preservation method has the advantages of improving in-vitro conservation efficiency, greatly reducing preservation cost and increasing the survival rate of oriental lilies.
Description
technical field [0001] The invention relates to the technical field of planting, in particular to a method for preserving oriental lilies. Background technique [0002] Lily, the scientific name (Lilium brownii var. viridulum Baker) is also known as Qiangshu, Fanjiu, Shandan, Daoxian, Zhongmai, Zhongting, Moro, Zhongfeng, Zhongfenghua, Lily Garlic, Dafu Garlic, Suannao, Nocturnal lily, etc., are perennial herbaceous bulbous plants of the genus Lilium (scientific name: Lilium) of the Liliaceae family. They are native to China and are mainly distributed in temperate regions of the northern hemisphere such as eastern Asia, Europe, and North America. At least 120 species have been found in the world, of which 55 species are produced in China. In recent years, many new species have been produced through artificial hybridization, such as Asian lily, perfume lily, and fire lily. The bulbs are rich in starch, edible and also used as medicine. [0003] Oriental lily is a perennial...
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