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Preservation method for gerbera jamesonii genetic resource

A preservation method and gerbera technology, applied in the field of preservation of gerbera germplasm resources, can solve the problems of high cost of seed conservation, loss of typicality of gerbera varieties, asexual variation of gerbera seeds and seedlings, etc., so as to improve the formation quantity and efficiency. , shorten the callus differentiation time and improve the efficiency of in vitro preservation

Inactive Publication Date: 2016-11-16
WUXI NANLIGONG TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, a kind of routine propagation method of gerbera is to adopt division method, but its propagation coefficient is low, can not meet the needs of large-scale production, and because factors such as culture temperature, the number of subgenerations of proliferating buds and hormone concentration are easy to Cause asexual variation in gerbera seedlings
[0005] At present, most germplasm resources of gerbera are preserved by indoor tissue culture and field cultivation. The disadvantage of indoor tissue culture preservation is that the subculture period is short and the cost of seed preservation is relatively high; while the defect of field cultivation preservation is that it occupies an area of More light, temperature, water, and pest control are difficult. Years of cultivation will cause soil compaction, salinization, and a large amount of pathogenic bacteria to accumulate, resulting in the loss of original typicality of gerbera varieties.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for preserving gerbera germplasm resources, the specific steps are as follows:

[0034] (1) Receptacle explant pretreatment:

[0035] Select flower buds with a diameter of 1-2 cm, refrigerate them at 4°C for 3 days, take them out and return them to room temperature, peel off the bracts and florets, wash them with pure water, and sterilize them in 0.05% mercuric chloride aqueous solution for 5 minutes, then Disinfect with 70% alcohol for 30 seconds, and then rinse with sterile water for 3 times to obtain the gerbera receptacle explant.

[0036] (2) Explant inoculation and induction culture:

[0037]Transfer the gerbera receptacle explants into the sterilized first culture medium, set the temperature at 23±1°C, use blue light as the light source, control the light intensity to 600Lx, and control the photoperiod to light 14h / dark 10h, callus was induced in about 10 days.

[0038] The composition of the first culture medium is: KNO 3 900mg / L, 2-amino-5-carboxy...

Embodiment 2

[0052] A method for preserving gerbera germplasm resources, the specific steps are as follows:

[0053] (1) Receptacle explant pretreatment:

[0054] Select flower buds with a diameter of 1-2 cm, refrigerate them at 4°C for 3 days, take them out and return them to room temperature, peel off the bracts and florets, wash them with pure water, and sterilize them in 0.05% mercuric chloride aqueous solution for 5 minutes, then Disinfect with 70% alcohol for 20 seconds, and then rinse with sterile water for 3 times to obtain gerbera receptacle explants.

[0055] The percentages mentioned above are volume percentages.

[0056] (2) Explant inoculation and induction culture:

[0057] Transfer the gerbera receptacle explants into the sterilized first medium, set the temperature at 23±1°C, use blue light as the light source, control the light intensity to 400Lx, and control the photoperiod to light 14h / dark 10h, callus was induced in about 10 days.

[0058] The composition of the fir...

Embodiment 3

[0073] A method for preserving gerbera germplasm resources, the specific steps are as follows:

[0074] (1) Receptacle explant pretreatment:

[0075] Select flower buds with a diameter of 2 cm, refrigerate them at 4°C for 5 days, take them out and return them to room temperature naturally, peel off the bracts and florets, wash them with pure water, sterilize in 0.05% mercuric chloride aqueous solution for 8 minutes, and then use 70 % alcohol for 40 seconds, and then rinsed with sterile water for 3 times to obtain the Gerbera chrysanthemum receptacle explants.

[0076] The percentages mentioned above are volume percentages.

[0077] (2) Explant inoculation and induction culture:

[0078] Transfer the gerbera receptacle explants into the sterilized first medium, set the temperature at 23±1°C, use blue light as the light source, control the light intensity to 800Lx, and control the photoperiod to light 14h / dark 10h, callus was induced in about 10 days.

[0079] The compositio...

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PUM

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Abstract

The invention discloses a preservation method for a gerbera jamesonii genetic resource. The preservation method comprises the six steps of gerbera jamesonii receptacle explant pretreatment, inoculation induction culture, transfer induction culture, germplasm separation preservation, recovery culture, and seedling acclimating and hardening. By means of the preservation method for the gerbera jamesonii genetic resource, the separation preservation time of the gerbera jamesonii genetic resource is remarkably prolonged, the separation preservation efficiency is remarkably improved, the preservation time of the gerbera jamesonii genetic resource is greatly prolonged, the gerbera jamesonii genetic resource can be preserved for a long time, and application prospects are achieved.

Description

technical field [0001] The invention relates to a method for preserving biological germplasm resources, in particular to a method for preserving gerbera germplasm resources. Background technique [0002] Gerbera is a perennial evergreen herbaceous flower of the genus Gerbera in the family Asteraceae. It is one of the top five cut flowers in the world. It is popular among people because of its large flowers, bright flowers, full color and long vase life. It is widely planted in my country. [0003] Since most gerbera are self-infertile, hybrid offspring will split and mutate. When genetic information is passed from parent to offspring, the variety of gerbera will degenerate and lose its original typical due to the cultivation environment such as light, water, temperature, nutrition and other reasons. It usually manifests as smaller flowers, more transitional colors, disordered flower shape, thinner flower stalks, lower resistance, and shorter flowering period. [0004] In th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/001A01H4/008
Inventor 许建中安剑石孟剑华
Owner WUXI NANLIGONG TECH DEV
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