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Artificial cultivation method of radix ranunculus ternate

A technology of artificial cultivation and cat's claw grass, applied in cultivation, soilless cultivation, horticultural methods, etc., can solve the problems of unsatisfactory medicinal effect, lack of effective ingredients, low root reproduction coefficient, etc., to overcome regional limitations and The cost disadvantage, the effect of increasing the number and efficiency of formation, and shortening the time of callus differentiation

Inactive Publication Date: 2016-11-23
WUXI NANLIGONG TECH DEV
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low cost of wild cat's claw and its commonness in some areas, Yaonong mostly adopts the method of artificial transplanting to cultivate cat's claw, but this method is only suitable for environments with a large number of wild cat's claws, not suitable for most areas
Root propagation is to use the method of cutting and planting the small root next to the root of cat's claw to realize artificial propagation. The coefficient of root propagation is low and cannot meet the needs of large-scale production. Factors such as hormone concentration can easily cause asexual variation in cat's claw seedlings
Ranunculaceae plants have great variability, and the cross-pollination of cat's claw will also lead to gene mixing. The seeds obtained by this method will not achieve the expected medicinal effect after continuous cultivation, and it is easy to cause palmitic acid Lack of active ingredients such as ethyl ester, myristic acid, palmitic acid, β-sitosterol, stigmasterol, and campesterol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of cat's claw in vitro cultivation method, concrete steps are as follows:

[0030] (1) Receptacle explant pretreatment:

[0031] Select the flower buds of cat’s claw, refrigerate them at 4°C for 3 days, take them out and let them return to room temperature naturally, peel off the bracts and florets, rinse them with pure water, sterilize them in 0.05% mercuric chloride aqueous solution for 5 minutes, and then use Disinfect with 70% alcohol for 30 seconds, and then rinse with sterile water for 3 times to obtain the explant of cat's claw torus; the percentages mentioned above are volume percentages.

[0032] (2) Explant inoculation and induction culture:

[0033] Transfer the explants of cat’s claw receptacles into the sterilized first medium, set the temperature at 23±1°C, use blue light as the light source, control the light intensity to 600Lx, and control the photoperiod to light 14h / dark 10h, the medium was changed every ten days until the callus was induced. ...

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PUM

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Abstract

The invention discloses an artificial cultivation method of radix ranunculus ternate. In terms of artificial cultivation of Chinese herbal medicines, a radix ranunculus ternate receptacle explant induction cultivation method is used for the first time, radix ranunculus ternate seedlings are obtained by differentiation induction cultivation, radix ranunculus ternate root tubers capable of serving as Chinese medicinal materials are obtained after domestication, acclimatization and transplanting cultivation, and excellent radix ranunculus ternate germplasm resources are further obtained. The radix ranunculus ternate seedlings obtained by callus differentiation cultivation overcome territorial limitation and cost disadvantages of artificial transplanting, also avoid the atypical property of genes of the germplasm resources obtained by seed propagation, and have an application prospect in the field of popularization of artificial cultivation of the radix ranunculus ternate.

Description

technical field [0001] The invention relates to an artificial cultivation method of cat's claw, in particular to an artificial cultivation method based on in vitro cultivation of cat's claw anthers. Background technique [0002] Cat's claw is a perennial herb of the family Ranunculaceae, whose roots are used as medicine. Mainly produced in Jiangsu, Zhejiang, Anhui, Jiangxi, Henan, Hubei, Guizhou, Guangxi and other regions. Cat's claw has a slightly sweet taste and mild nature, enters the lung and liver meridians, and has the functions of dispelling stagnation, reducing swelling, clearing away heat, and detoxifying. Indications for tuberculosis, neck scrofula, pharyngitis, hemorrhoids, etc. It can also treat a variety of cancers, lymphoma, etc., with remarkable anti-cancer effects and high cure rate. [0003] Because cat's claw is wild, it is easy to dig and has a wide range of curative effects, the market demand is large, and the price is relatively high, which has caused ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00C05G1/00
CPCA01H4/008A01G24/23A01G31/00A01H4/001C05C9/00C05G1/00C05C11/00C05D1/00C05D9/02C05D9/00C05F11/00C05F11/02
Inventor 林少会许建中孟剑华
Owner WUXI NANLIGONG TECH DEV
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