Cultivation method of Gerbera detoxified seedlings

A cultivation method and technology of gerbera, applied in the cultivation field of virus-free seedlings of gerbera, can solve the problems of low seedling survival rate in transplanting, low seedling proliferation efficiency, poor quality of induced seedlings, etc., shorten callus differentiation time, improve Seedling growth rate, the effect of increasing the number and efficiency of formation

Inactive Publication Date: 2016-11-09
WUXI NANLIGONG TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tissue culture method of Gerbera chrysanthemum in the prior art has problems such as low tissue induction rate, poor quality of induced seedlings and many weak seedlings during indoor cultivation, while there are also problems such as low seedling proliferation efficiency and low seedling transplanting survival rate in the process of outdoor cutting culture And other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for cultivating virus-free seedlings of gerbera, the specific steps are as follows:

[0030] (1) Receptacle explant pretreatment:

[0031] Select flower buds with a diameter of 1-2 cm, refrigerate them at 4°C for 3 days, take them out and return them to room temperature, peel off the bracts and florets, wash them with pure water, and sterilize them in 0.05% mercuric chloride aqueous solution for 5 minutes, then Disinfect with 70% alcohol for 30 seconds, and then rinse with sterile water for 3 times to obtain the gerbera receptacle explant.

[0032] (2) Explant inoculation and induction culture:

[0033] Transfer the gerbera receptacle explants into the sterilized first culture medium, set the temperature at 23±1°C, use blue light as the light source, control the light intensity to 600Lx, and control the photoperiod to light 14h / dark 10h, callus was induced in about 10 days.

[0034] The composition of the first culture medium is: KNO 3 900mg / L, 2-amino-5-c...

Embodiment 2

[0043] A method for cultivating virus-free seedlings of gerbera, the specific steps are as follows:

[0044] (1) Pretreatment of receptacle explants: select flower buds with a diameter of 1-2 cm, refrigerate at 4°C for 3 days, take out the flower buds and return them to room temperature naturally, peel off the bracts and florets, rinse with pure water, first in 0.05 % mercuric chloride aqueous solution for 5 minutes, then sterilized with 70% alcohol for 20 seconds, and then rinsed with sterile water for 3 times to obtain the gerbera receptacle explants. The percentages mentioned above are volume percentages.

[0045] (2) Explant inoculation and induction culture: transfer the gerbera receptacle explants into the sterilized first medium, set the temperature at 23±1°C, use blue light as the light source, and control the light intensity. The temperature is 400Lx, the photoperiod is controlled as light 14h / dark 10h, and the callus is induced in about 10 days.

[0046] The compos...

Embodiment 3

[0054] A method for cultivating virus-free seedlings of gerbera, the specific steps are as follows:

[0055] (1) Receptacle explant pretreatment:

[0056] Select flower buds with a diameter of 2 cm, refrigerate them at 4°C for 5 days, take them out and return them to room temperature naturally, peel off the bracts and florets, wash them with pure water, sterilize in 0.05% mercuric chloride aqueous solution for 8 minutes, and then use 70 % alcohol for 40 seconds, and then rinsed with sterile water for 3 times to obtain the Gerbera chrysanthemum receptacle explants.

[0057] The percentages mentioned above are volume percentages.

[0058] (2) Explant inoculation and induction culture:

[0059] Transfer the gerbera receptacle explants into the sterilized first medium, set the temperature at 23±1°C, use blue light as the light source, control the light intensity to 800Lx, and control the photoperiod to light 14h / dark 10h, callus was induced in about 10 days.

[0060] The compo...

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PUM

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Abstract

The invention discloses a cultivation method of Gerbera detoxified seedlings. The cultivation method includes steps of receptacle explant pretreatment of Gerbera, inoculation and induced culture, transfer induced culture, seedling domestication and seedling hardening. By using the cultivation method, interference caused by virus and disease factors during cultivation is avoided, forming number and efficiency of callus are increased, culture period is short, and seedling qualification rate is increased; the cultivation method has wide application prospect in the field of Gerbera tissue culture.

Description

technical field [0001] The invention relates to a method for cultivating virus-free seedlings of gerbera, in particular to a method for cultivating virus-free seedlings based on explants of gerbera receptacles. Background technique [0002] Gerbera is a perennial evergreen herbaceous flower of the genus Gerbera in the family Asteraceae. It is one of the top five cut flowers in the world. It is popular among people because of its large flowers, bright flowers, full color and long vase life. It is widely planted in my country. [0003] Since most gerbera are self-infertile, hybrid offspring will split and mutate. When genetic information is passed from parent to offspring, the variety of gerbera will degenerate and lose its original typical due to the cultivation environment such as light, water, temperature, nutrition and other reasons. It usually manifests as smaller flowers, more transitional colors, disordered flower shape, thinner flower stalks, lower resistance, and shor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 许建中林少会孟剑华
Owner WUXI NANLIGONG TECH DEV
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