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In-vitro cultivation method of Saussurea involucrata

A cultivation method and technology of snow lotus, applied in horticultural methods, botanical equipment and methods, gardening, etc., can solve problems such as asexual variation of snow lotus seedlings, failure to achieve expected medicinal effects, and uncontrollability, so as to increase the number of seeds formed and efficiency, shorten callus differentiation time, shorten the effect of cultivation period

Inactive Publication Date: 2016-11-09
WUXI NANLIGONG TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reproduction coefficient of the tissue culture method is low, which cannot meet the needs of large-scale production, and due to factors such as light, water, temperature, nutrition, and hormone concentration in the culture environment, it is easy to cause asexual variation in the snow lotus seedlings.
[0005] There are also many defects in the method of seed propagation. Because the snow lotus grows in an extreme environment, its seeds have the characteristics of strong vitality and slow germination. The seeds of some snow lotus can be buried in the ground for three years without germination. Controlling greatly prolongs the cycle of artificial cultivation of snow lotus; if artificial activation of snow lotus seeds is used, the biological structure of the seeds may be destroyed, resulting in a waste of germplasm resources of snow lotus; Mixed, the obtained seeds cannot achieve the expected medicinal effect after continued cultivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A kind of snow lotus culture method in vitro, concrete steps are as follows:

[0025] (1) Receptacle explant pretreatment:

[0026] Select the flower buds of snow lotus, refrigerate them at 4°C for 3 days, take them out and let them return to room temperature naturally, peel off the bracts and florets, wash them with pure water, sterilize them in 0.05% mercuric chloride aqueous solution for 5 minutes, and then use 70% Alcohol disinfection for 30 seconds, followed by rinsing with sterile water for 3 times to obtain the explants of snow lotus receptacle; the percentages mentioned above are volume percentages.

[0027] (2) Explant inoculation and induction culture:

[0028] Move the explants of snow lotus receptacle into the sterilized first medium, set the temperature at 23±1°C, use blue light as the light source, control the light intensity to 600Lx, and control the photoperiod to light 14h / dark 10h, The medium was changed every ten days until callus was induced.

[0...

Embodiment 2

[0038] A kind of cultivation method of snow lotus virus-free seedling, concrete steps are as follows:

[0039] (1) Pretreatment of receptacle explants: select the flower buds of snow lotus, refrigerate them in 4°C for 3 days, take out the flower buds and return them to room temperature naturally, peel off the bracts and florets, wash them with pure water, and put them in 0.05% l Sterilized in mercury aqueous solution for 5 minutes, then sterilized with 70% alcohol for 20 seconds, and then rinsed with sterile water for 3 times to obtain the explant of Saussurea sinensis, and the above-mentioned percentages are volume percentages.

[0040] (2) Explant inoculation and induction culture: transfer the explants of snow lotus receptacle into the sterilized first medium, set the temperature at 23±1°C, use blue light as the light source, and control the light intensity at 400Lx , the photoperiod was controlled as light 14h / dark 10h, and the medium was changed every ten days until the c...

Embodiment 3

[0049] A kind of cultivation method of snow lotus virus-free seedling, concrete steps are as follows:

[0050] (1) Receptacle explant pretreatment:

[0051] Select the flower buds of snow lotus, refrigerate them at 4°C for 5 days, take them out and let them return to room temperature naturally, peel off the bracts and florets, wash them with pure water, sterilize them in 0.05% mercuric chloride aqueous solution for 8 minutes, and then use 70% Alcohol disinfection for 40 seconds, followed by rinsing with sterile water for 3 times to obtain Saussurea receptacle explants.

[0052] The percentages mentioned above are volume percentages.

[0053] (2) Explant inoculation and induction culture:

[0054] Move the explants of snow lotus receptacle into the sterilized first medium, set the temperature at 23±1°C, use blue light as the light source, control the light intensity to 800Lx, and control the photoperiod to light 14h / dark 10h, The medium was changed every ten days until callu...

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Abstract

The invention discloses an in-vitro cultivation method of Saussurea involucrata. The in-vitro cultivation method is characterized in that the receptacle explant of the Saussurea involucrata is used to perform induction cultivation, blue light irradiation is performed first, then fluorescent light irradiation is performed, and the combination of the blue light irradiation and the fluorescent light irradiation greatly shortens the callus differentiation time and increases the callus formation number and efficiency. The in-vitro cultivation method has the advantages that the cultivation cycle is shortened, the typicality of Saussurea involucrata genes is kept, and the method is promising in application prospect in the field of artificial Saussurea involucrata cultivation.

Description

technical field [0001] The invention relates to an in vitro cultivation method of rare Chinese herbal medicine, in particular to an in vitro cultivation method based on snow lotus anther. Background technique [0002] Snow lotus is a plant of the genus Asteraceae in the family Asteraceae, generally referring to snow lotus, a perennial herb containing protein, amino acids, flavonoids, alkaloids, etc. and other effects. It has high medicinal value and can be used for numbness of the extremities caused by wind-cold, rheumatoid arthritis, cold pain in the lower abdomen, irregular menstruation in women, low back pain due to kidney deficiency, etc. [0003] Snow lotus mostly grows near the snow line above 3,000 meters above sea level in Tianshan, Altai and Kunlun Mountains, and the growth period takes 6 to 8 years. At the end of the 20th century, the demand for snow lotus at home and abroad was increasing day by day, and the wild snow lotus was excavated wildly, resulting in a s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 许建中孟剑华安剑石
Owner WUXI NANLIGONG TECH DEV
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