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Method for evaluating blood coagulation reaction

A technology for coagulation and coagulation factors, which is used in biochemical equipment and methods, microbial determination/inspection, instruments, etc.

Active Publication Date: 2015-09-23
CHUGAI PHARMA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the existing FVIII drug efficacy monitoring methods may not be applicable

Method used

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  • Method for evaluating blood coagulation reaction
  • Method for evaluating blood coagulation reaction
  • Method for evaluating blood coagulation reaction

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Experimental program
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Effect test

Embodiment Construction

[0086] Hereinafter, the present invention is described in more detail through examples, but the present invention is not limited to these examples.

[0087] Preparation of anti-FIXa / FX bispecific antibody with FVIII functional substitution activity and determination of FVIII functional substitution activity

[0088] Anti-FIXa / FX bispecific antibodies with FVIII function substitution activity were obtained using the methods described in WO2005 / 035756, WO2006 / 109592, and WO2012 / 067176. The antibody genes described in WO2005 / 035756, WO2006 / 109592, and WO2012 / 067176 were incorporated into animal cell expression vectors, which were transfected into HEK293 cells to transiently express bispecific antibodies. Subsequently, the bispecific antibody contained in the cell culture supernatant was purified with protein A and filtered.

[0089] The FVIII function substitution activity of thus purified bispecific antibodies was determined by the enzymatic assay shown below. At room temper...

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Abstract

Various methods for evaluating hemostatic effect and various blood coagulation initiation reagents were studied to construct a method for evaluating blood coagulation reaction mediated by a substance having an activity of substituting for coagulation factor VIII (FVIII). As a result, it was discovered that by using a coagulation initiation reagent containing activated coagulation factor XI (FXIa) and phospholipids, the effect of a substance having an activity of substituting for coagulation factor VIII (FVIII) on blood coagulation reaction can be evaluated using the amount of thrombin generated in the blood sample as an indicator.

Description

technical field [0001] The present invention relates to a method for evaluating coagulation reactions mediated by substances having coagulation factor VIII (FVIII) function substitution activity. In addition, the present invention relates to a reagent for evaluating blood coagulation reaction comprising activated blood coagulation factor XI (FXIa), and a kit for evaluating blood coagulation reaction mediated by a substance having blood coagulation factor VIII (FVIII) function substitution activity comprising the reagent Wait. Background technique [0002] Hemophilia is a bleeding disorder caused by congenital deficiency or insufficiency of coagulation factor VIII (FVIII) or coagulation factor IX (FIX). The former is called hemophilia A and the latter is called hemophilia B. Their genes are all present on the X chromosome, and genetic abnormalities are transmitted through sex-linked recessive inheritance, so more than 99% of the patients are male. The known prevalence is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/86
CPCG01N2800/224G01N2333/755G01N33/86C12Q1/56C12N9/6443G01N2333/96452
Inventor 添田哲弘北泽刚久岛绿伦
Owner CHUGAI PHARMA CO LTD