Method for maintaining heterogeneous concentrations of molecules in emulsion droplets

A technology of molecules and emulsions, applied in the field of microfluidics

Inactive Publication Date: 2015-11-11
GNUBIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] There is a problem: while some relatively large entities (e.g. some cells and proteins) can be encapsulated in droplets and stored stably (e.g., droplets retain their original composition and concentration) for days, weeks, months and / or or longer without molecular leakage or exchange with neighboring droplets in the population, but some molecules and / or molecules can transfer across emulsion droplet boundaries (referred to in the text as "interfaces") and will transfer and mixing

Method used

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  • Method for maintaining heterogeneous concentrations of molecules in emulsion droplets
  • Method for maintaining heterogeneous concentrations of molecules in emulsion droplets
  • Method for maintaining heterogeneous concentrations of molecules in emulsion droplets

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Embodiment Construction

[0025] The present invention generally relates to methods of maintaining a heterogeneous concentration of metastatic target molecules in emulsion droplets by using one of several means to bind them to other non-transferable molecules in droplets contained in a microfluidic device. accomplish.

[0026] In an embodiment of the invention, the emulsion droplets are located in a microfluidic device. As used herein, a "microfluidic device" is a device capable of exerting a deterministic function on small quantities of liquid or gaseous fluids, typically measured in volume (e.g., milliliter (mL), microliter (μL), nanoliter (nL), picoliter (pL) or femtoliter (fL) volume) and / or by physical measure (such as millimeter (mm), micrometer (μm) (also known as "millionth of a meter"), nanometer (nm ) etc.) to measure. Functions may include mixing, disintegrating, sorting, heating, etc. Microfluidic devices may contain microfluidic channels as a means of transferring fluid or sample from o...

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Abstract

The present invention provides a method for maintaining heterogeneous concentrations of transitory target molecules in emulsion droplets by using one of a number of means to associate them to other, non-transitory molecules within the emulsion droplets contained in a microfluidic device. The present invention further provides a method for ensuring that the concentration and speciation of molecules will remain constant when stored in emulsion droplets. This results in emulsion droplets having heterogeneous concentrations of molecules, thereby improving the performance and applicability of droplet-based microfluidics.

Description

[0001] Related Applications and Incorporation by Reference [0002] This application claims priority to US Provisional Patent Application Serial No. 61 / 737,625, filed December 14, 2012. [0003] The above application and all documents cited herein or during its prosecution ("Application Cited Documents") and all documents cited or referenced in the Application Cited Documents ("herein cited documents") and all documents cited or referenced in the herein cited documents The documents used, together with any manufacturer's instructions, descriptions, product specifications and product pages for any product described herein or any document incorporated by reference herein, are hereby incorporated by reference and may be used in the practice of the present invention. More specifically, all references are herein incorporated by reference to the same extent as if each individual reference was specifically and individually indicated to be incorporated by reference. technical field ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/10
CPCB01J19/0046A61K9/107B01J2219/00599B01J2219/00722C40B50/08
Inventor T·亨J·布罗沃夫S·其阿尼
Owner GNUBIO
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