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Method and device for batch identification of wheat salt tolerance

A wheat salt tolerance and batch technology, applied in the field of identification of plant salt tolerance, can solve the problems of cumbersome steps, the inability to eliminate differences in cultivation conditions such as light and temperature, and the inability of hydroponic conditions to simulate the growth environment of wheat seedlings. Consistency, strong practicability, and the effect of ensuring uniformity

Inactive Publication Date: 2016-03-02
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the identification of salt tolerance in hydroponics can make wheat seedlings quickly develop salt stress phenotypes, which can partially reflect the salt tolerance characteristics of wheat seedlings, but a single hydroponic condition cannot simulate the normal growth environment of wheat seedlings, and there are many shortcomings
For example, since the base of the seedling grows in the hole of the bubble film, the tight space will limit the tillering and growth of wheat
Moreover, since the wheat seedlings cannot change the planting position once transplanted into the bubble film board, it is impossible to eliminate the differences in the cultivation conditions such as light and temperature, and ensure the consistency of the experimental conditions of each individual plant
In addition, the steps of this method are relatively cumbersome, and the seedlings need to be transplanted before hydroponics. When the seedlings grow up, they will fall due to lack of support, and must be transplanted into soil culture conditions again to reproduce.

Method used

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  • Method and device for batch identification of wheat salt tolerance
  • Method and device for batch identification of wheat salt tolerance
  • Method and device for batch identification of wheat salt tolerance

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1, the device of batch identification wheat salt tolerance

[0033] Such as figure 1 Shown, the structural representation (only showing the setting of the 1st row and the 1st row of plastic tubes) of the device of batch identification wheat salt tolerance provided by the present invention, it comprises the plastic box 1 (long 65cm * wide of an upper end opening) 40cm × height 16cm), set up a 98-hole (7 × 14) tube rack 2 (U.S. Stuewe & Sons company, model: RL98) in this plastic box 1, place a tapered plastic tube 3 (its tube) in each hole Mouth diameter is 3.8cm, and length is 21cm, and volume is 164ml, U.S. Stuewe & Sons company, model SC10), the two ends openings of plastic tube 3, its small mouth end is arranged downwards, and the lower end opening of plastic tube 3 and the bottom of plastic box 1 There is a distance between them, which can be adjusted according to different experimental requirements (the distance is about 1 cm).

[0034] In the device of...

Embodiment 2

[0035] Embodiment 2, identify the salt tolerance of wheat

[0036] In this example, wheat varieties Xiaoyan 60 and Jimai 22 were selected as test materials.

[0037] Wheat variety Jimai 22 is a new super-high-yield, multi-resistant, and high-quality medium-gluten wheat variety newly bred by the Crop Research Institute of Shandong Academy of Agricultural Sciences. It passed the approval of Shandong Province and the National Huanghuai North Area in September 2006 and January 2007 respectively. The approval number They are Lunong Shen 2006050 and Guo Shen Mai 2006018 respectively.

[0038] Wheat variety Xiaoyan 60 is a new drought-resistant, salt-tolerant, high-yielding, high-quality wheat variety cultivated by the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences. It has applied for and obtained the right to protect new varieties, application number: 20100586.6.

[0039]Fill the plastic pipe 3 in the device of Example 1 with soil, and add the nutrient...

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Abstract

The invention discloses a method and device for identification of plant salt tolerance. The device comprises a box body of which the upper end is open, wherein a plurality of pipelines are disposed in the box body, the positions of the pipelines are adjustable, and two ends of each pipeline are open; and the pipelines are disposed in the box body by a pipe frame, and a spacing is formed between the lower end opening of each pipeline and the bottom part of the box body. The method for the batch identification of wheat salt tolerance with application of the device comprises the steps that (1) soil is put in the pipelines in the device; (2) seeds of to-be-identified wheat are planted in the pipelines; (3) the pipelines are placed in the box body by the pipe frame, wherein a nutrient solution is stored in the box body, and the lower ends of the pipelines are soaked in the nutrient solution; and (4) the batch identification of the wheat salt tolerance can be realized through had justment and control of the salt content of the nutrient solution. The method is widely applicable to the salt tolerance identification of wheat germplasm resources, genetic materials and new varieties (species).

Description

technical field [0001] The invention relates to a method and a device for identifying the salt tolerance of plants, in particular to a method and a device for identifying the salt tolerance of wheat in batches. Background technique [0002] Soil salinization is a worldwide resource and ecological problem. According to statistics, the area of ​​saline soil in the world is 954.38 million hectares, and saline soil is widely distributed in my country, with a total area of ​​1.487 billion mu (about 100 million hectares). Rational use of salinized soil resources is an urgent problem in my country's agricultural development. There are many ways to develop and utilize salinized soil, and planting salt-tolerant crop varieties is one of the cost-effective ways. Wheat is the second largest food crop in the world, and it is also one of the main cultivated crops in saline soil in my country. Soil salinization seriously affects wheat yield. Therefore, breeding salt-tolerant wheat varie...

Claims

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Application Information

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IPC IPC(8): A01G7/00A01G9/02
CPCA01G7/00A01G9/02
Inventor 郑琪滕婉李宏伟李滨李振声
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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