Method for distinguishing Hainan and Guizhou province germplasm Blumea balsamifera

A kind of Aina incense and germplasm technology, applied in the analysis of materials, preparation of test samples, material analysis by optical means, etc., can solve the problems of complex operation, difficult to identify plant germplasm in different regions, and complicated steps. , to achieve the effect of simple operation

Active Publication Date: 2016-04-20
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new technology helps identify plants that are producing citronella oil from their seeds by measuring certain characteristics such as these properties at specific points during plant growth stages. By doing this it makes possible quicker and more accurate identification than previous methods like chemical analysis which only showed how well they were growing on them.

Problems solved by technology

Technological Problem: Prior Art describes methods that were developed by researchers at various locations around China during their time-consuming process of studying plants with specific characteristics or compositions. These technical problem addressed by this patents include difficulty in accurately differentiating between different types of vegetables due to lack of easy accessibility across multiple gaps within these fields.

Method used

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  • Method for distinguishing Hainan and Guizhou province germplasm Blumea balsamifera
  • Method for distinguishing Hainan and Guizhou province germplasm Blumea balsamifera
  • Method for distinguishing Hainan and Guizhou province germplasm Blumea balsamifera

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Experimental program
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Effect test

Embodiment 1

[0036] A number of mature leaves with normal growth and development of Hainan germplasm and Guizhou germplasm were randomly selected, divided into small pieces of 1cm×1cm, quickly put into FAA fixative solution, fixed for 24h, serial alcohol dehydration, infiltration, embedding, German Leica RM2245 microtome Sliced ​​with a thickness of 8 μm, stained with safranin-fast green, mounted with neutral gum, observed, photographed, and measured the thickness of the leaves of Ainarbaum with a DMI4000B digital optical microscope imaging system from Germany.

[0037] 15 samples were randomly selected from Hainan germplasm and Guizhou germplasm group, and SPSS19.0 software was used for statistical analysis to calculate the average value and standard error of leaf thickness. The test was conducted to analyze the significant difference between the two groups of data (P<0.05), and the results are shown in Tables 1-7.

[0038] According to the results of microscopic measurement and statistic...

Embodiment 2

[0055] A number of mature leaves with normal growth and development of Hainan germplasm and Guizhou germplasm were randomly selected, divided into small pieces of 1cm×1cm, quickly put into FAA fixative solution, fixed for 24h, serial alcohol dehydration, infiltration, embedding, German Leica RM2245 microtome Slices with a thickness of 8 μm, stained with safranin-fast green, mounted with neutral gum, observed, photographed, and measured the thickness of the upper epidermis of the leaves of Aina japonica (Leica) DMI4000B digital optical microscopic imaging system.

[0056]15 samples were randomly selected from Hainan germplasm and Guizhou germplasm group, and SPSS19.0 software was used for statistical analysis to calculate the average value and standard error of upper epidermis thickness, and the results were expressed in the form of "mean ± standard error". The t test was used to analyze the significant difference between the two groups of data (P<0.05), and the results are show...

Embodiment 3

[0066] A number of mature leaves with normal growth and development of Hainan germplasm and Guizhou germplasm were randomly selected, divided into small pieces of 1cm×1cm, quickly put into FAA fixative solution, fixed for 24h, serial alcohol dehydration, infiltration, embedding, German Leica RM2245 microtome Slices with a thickness of 8 μm, stained with safranin-fast green, mounted with neutral gum, observed, photographed, and measured the thickness of palisade tissue and spongy tissue in Leica (Leica) DMI4000B.

[0067] 15 samples were randomly selected from Hainan germplasm and Guizhou germplasm group, and SPSS19.0 software was used for statistical analysis to calculate the average value and standard error of palisade tissue and spongy tissue thickness, and the results were expressed in the form of "mean ± standard error" The t test was used to analyze the significant difference between the two groups of data (P<0.05), and the results are shown in Tables 11-13.

[0068] Acco...

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Abstract

The present invention relates to a method for distinguishing Hainan and Guizhou province germplasm Blumea balsamifera, by measurement of one or more of leaf tip part section thickness, little middle section thickness, leaf base section thickness, leaf epidermal thickness, palisade tissue thickness, spongy tissue thickness or palisade tissue to spongy tissue ratio of Blumea balsamifera to be tested and of comparison of measurements results and standard numerical values, the Hainan and Guizhou province germplasm Blumea balsamifera can be effectively distinguished. The method is simple in operation, and can fast and effectively distinguish the Hainan and Guizhou province germplasm Blumea balsamifera, and a scientific basis and reference for cultivation, directed breeding or medicine production of Blumea balsamifera derived from different germplasm is provided.

Description

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Claims

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Application Information

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OwnerTROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI