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A method for judging the degree of fluorescence elimination of Raman spectrum by information entropy

A technology for Raman spectroscopy and fluorescence elimination, applied in Raman scattering, material excitation analysis, etc., can solve the problems of increasing instrument cost and complexity, instrument design limitations, and untrue results, etc., to increase equipment cost, eliminate methods, The effect of simple equipment

Active Publication Date: 2018-10-19
OPTOTRACE SUZHOU TECH
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a method for judging the degree of fluorescence elimination of Raman spectrum through information entropy, so as to solve the existing problems of increasing instrument cost and complexity, untrue results, and constituting restrictions on instrument design. Inadequacies

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  • A method for judging the degree of fluorescence elimination of Raman spectrum by information entropy
  • A method for judging the degree of fluorescence elimination of Raman spectrum by information entropy
  • A method for judging the degree of fluorescence elimination of Raman spectrum by information entropy

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Embodiment 1

[0049] A method for judging the degree of fluorescence elimination of sucrose Raman spectrum by information entropy, the method comprising the following steps:

[0050] A. Use Raman laser probe 785nm laser excitation, measure the Raman spectrum of sucrose, fix the focus, set the integration time to 10s, measure twice at a time interval of 30s, and record the resulting mixed signal S 1、 S 2 ,like figure 1 as shown in S 1 is the measured spectrum at time 1, S 2 is the measured spectrum at time 2;

[0051] B. Adjust the weight of the spectrum at time 2 to 1.028, subtract it from the spectrum at the previous time, and obtain the fluorescence drop value Δ that effectively eliminates the Raman component in it S , the weighted and subtracted spectra are respectively S 3 Spectra with unweighted direct subtraction S 4 For example figure 2 shown, from figure 2 It can be seen that the Raman component can be effectively eliminated by using weighted subtraction;

[0052] ...

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Abstract

The invention discloses a method for judging the fluorescence quenching degree of Raman spectrum through information entropy and relates to a Raman spectrum fluorescence quenching method. The method uses the discrete-sequence information entropy as the criterion of fluorescence deduction, fluorescence is gradually deducted from a mixed signal, and the fluorescence in the mixed signal is completely quenched when the information entropy reaches the minimum. The method has the advantages that the fluorescence quenching degree is judged through the information entropy, the method is scientific and reasonable and unlimited by instrument wave-number range, instrument performance can be brought into full play, and the freedom degree of instrument design and manufacturing is increased; the limitation that the fluorescence residue situation needs to be judged in a specific wavelength range in the prior art is solved, no special requirement on spectrum range is needed, and the method is adaptive to instrument design with different wavelength settings; Raman true signals which conform to actual mechanisms and are not interfered by fluorescence can be obtained fast and accurately while equipment complexity is not increased and an existing measuring habit is not changed, and the method is easy to popularize and use.

Description

technical field [0001] The invention relates to a method for eliminating fluorescence of Raman spectrum, in particular to a method for judging the degree of fluorescence elimination of Raman spectrum through information entropy. Background technique [0002] Laser Raman spectroscopy can provide molecular spectral information, and has the advantages of not being easily disturbed by water, non-contact and non-destructive samples, etc. It is a rapidly developing qualitative and quantitative analysis method. However, the simultaneous fluorescence generated by Raman scattered excitation interferes with the Raman spectral discernibility and signal quality. [0003] Micro-spectroscopic Raman spectrometers using array light-sensitivity have developed rapidly in recent years. The advantages are simple structure and no complicated maintenance. However, the use of hardware to eliminate fluorescence interference will increase the complexity of the equipment. For example, using the mobil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 姚志湘粟晖刘柳
Owner OPTOTRACE SUZHOU TECH
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