Method capable of improving accuracy of L-hydroxyproline Raman spectral detection

A Raman spectrum detection, hydroxyproline technology, applied in Raman scattering, preparation of test samples, measurement devices, etc., can solve problems such as misjudgment, affecting the accuracy of L-hydroxyproline, etc. Influence, Guaranteed Accuracy, Accurate Spectrogram Effect

Inactive Publication Date: 2018-06-29
LUOYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the prior art detects whether L-hydroxyproline is contained in the milk powder and usually adopts the Raman spectrum detection method, mainly after the milk powder is compressed into tablets, and then measures its Raman spectrum, and then compares the obtained Raman spectrum with the The Raman spectrum of L-hydroxyproline is compared, and finally through the comparison of the two spectra, it is determined whether L-hydroxyproline is added to the milk powder, but when detecting L-hydroxyproline, it may be due to grinding Other factors make L-hydroxyproline contain other impurities, which affect the accuracy of L-hydroxyproline Raman spectrum detection, resulting in wrong judgments when judging whether L-hydroxyproline is added to milk powder

Method used

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  • Method capable of improving accuracy of L-hydroxyproline Raman spectral detection

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Embodiment 1

[0026] A method capable of improving the detection accuracy of L-hydroxyproline Raman spectrum, comprising the following steps:

[0027] a. Dry the sample powder and potassium bromide in an oven for 20 minutes, and store them separately;

[0028] b. Take an appropriate amount of sample and place it in a mortar for grinding to obtain sample powder for subsequent use;

[0029] C, the potassium bromide dried in the step a is placed in a mortar and ground, and when grinding potassium bromide, apply force evenly in the same direction, and the gained is recorded as potassium bromide powder;

[0030] d. Mix the sample powder obtained in step b with the potassium bromide powder obtained in step c, and ultrasonically disperse for 40s to obtain the mixed powder;

[0031] e, compressing the mixed powder obtained in step d with a tablet press;

[0032] f. Place the pressed tablet in a Raman spectrometer, at 100-3700cm -1 Scan the Raman spectrum within the range to obtain the Raman spec...

Embodiment 2

[0040] A method capable of improving the detection accuracy of L-hydroxyproline Raman spectrum, comprising the following steps:

[0041] a. Dry the sample powder and potassium bromide in an oven for 25 minutes, and store them separately;

[0042] b. Take an appropriate amount of sample and place it in a mortar for grinding to obtain sample powder for subsequent use;

[0043] C, the potassium bromide dried in the step a is placed in a mortar and ground, and when grinding potassium bromide, apply force evenly in the same direction, and the gained is recorded as potassium bromide powder;

[0044] d. Mix the sample powder obtained in step b with the potassium bromide powder obtained in step c, and ultrasonically disperse for 60 seconds to obtain the mixed powder;

[0045] e, compressing the mixed powder obtained in step d with a tablet press;

[0046] f. Place the pressed tablet in a Raman spectrometer, at 100-3700cm -1 Scan the Raman spectrum within the range to obtain the Ram...

Embodiment 3

[0054] A method capable of improving the detection accuracy of L-hydroxyproline Raman spectrum, comprising the following steps:

[0055] a. Dry the sample powder and potassium bromide in an oven for 30 minutes, and store them separately;

[0056] b. Take an appropriate amount of sample and place it in a mortar for grinding to obtain sample powder for subsequent use;

[0057] C, the potassium bromide dried in the step a is placed in a mortar and ground, and when grinding potassium bromide, apply force evenly in the same direction, and the gained is recorded as potassium bromide powder;

[0058] d. Mix the sample powder obtained in step b with the potassium bromide powder obtained in step c, and ultrasonically disperse for 80s to obtain the mixed powder;

[0059] e, compressing the mixed powder obtained in step d with a tablet press;

[0060] f. Place the pressed tablet in a Raman spectrometer, at 100-3700cm -1 Scan the Raman spectrum within the range to obtain the Raman spec...

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Abstract

The invention relates to the technical field of Raman spectral detection, and in particular, relates to a method capable of improving the accuracy of L-hydroxyproline Raman spectral detection. With addition of potassium bromide, the fluorescence produced by other substances in a sample can be eliminated, the occurrence of a phenomenon that a Raman spectrum is flooded by the fluorescence emitted bythe substances is avoided, interference of other substances to experiments is avoided, and the accuracy of the detection is ensured. In addition, the force is uniformly applied in a same direction during grinding of potassium bromide, and the crystal transformation phenomenon of potassium bromide in the grinding process can be avoided, so as to ensure that the results are not affected; potassiumbromide and a sample powder are mixed with the mass ratio of (98-103):1, the influence of potassium bromide on the detection of the sample powder can be reduced to the most extent, and an obtained spectrogram is more accurate.

Description

technical field [0001] The invention relates to the technical field of Raman spectrum detection, in particular to a method capable of improving the detection accuracy of L-hydroxyproline Raman spectrum. Background technique [0002] Milk powder is a powder made by removing water from the fresh milk of mammals. It generally refers to the powder made from cow's milk through disinfection, degreasing, dehydration, drying and other processes; it can also refer to the milk of other animals such as goats. Products produced from milk. Milk powder is suitable for storage, easy to carry, and rich in nutrition. At the same time, it has good wettability, high dispersion, easy preparation, and can be dissolved quickly even with warm water, so it is favored by consumers. There are many types of milk powder, among which the formula and process of infant milk powder are the most particular. It is to meet the nutritional needs of infants and is closer to the composition of breast milk. On t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/28
CPCG01N1/28G01N1/286G01N21/65G01N2001/2866
Inventor 陶亚萍韩礼刚韩运侠胡要花李瑞
Owner LUOYANG NORMAL UNIV
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