Raman Spectroscopy Fluorescence Defluorescence Method Based on Kurtosis Judgment to Obtain Fluorescence Fading Measurement Value

Abstract

The invention relates to a Raman spectrum fluorescence eliminating method, in particular to a Raman spectrum fluorescence eliminating method for judging and obtaining a fluorescence fading measured value through kurtosis. According to the method, through desynchrony of Raman and fluorescence signal changes in mixed signals at different measuring moments, subtraction coefficients are adjusted, the subtraction value between spectral data at the moments before and after a series is calculated, the smallest subtraction value of kurtosis is selected and serves as the fluorescence fading measured value, the fluorescence fading measured value is subjected to noise reduction, the fluorescence fading measured value obtained after noise reduction serves as fluorescence components in the mixed signals, the fluorescence components are deducted from the mixed signals gradually, and finally pure Raman signals without fluorescence interference are obtained. Fluorescence of a Raman spectrum can be truly and effectively eliminated, measuring time can be shortened, signal acquisition and processing difficulty can be lowered, adopted equipment is simple, manufacturing cost is low, and application and popularization are easy.

Description

technical field [0001] The invention relates to a Raman spectrum defluorescence method, in particular to a Raman spectrum defluorescence method for obtaining a fluorescence fading measurement value through kurtosis judgment. Background technique [0002] Laser Raman spectroscopy is a rapidly developing qualitative and quantitative method, which can provide molecular spectral information similar to mid-infrared, and has the advantages of not being easily interfered by moisture, non-contact and non-destructive samples. However, when the laser stimulates Raman scattering, it also excites fluorescence. Fluorescence is the most important factor that interferes with the identifiability of Raman spectra and affects the quality of Raman signals. [0003] The fastest-growing miniature spectroscopic Raman spectrometer in recent years has the characteristics of simple structure and no complicated maintenance, but the use of hardware to eliminate fluorescence interference will increase ...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a Raman spectrum defluorescence method for obtaining the fluorescence fading measurement value through kurtosis judgment, so as to solve the problems existing in the prior art that it is difficult to eliminate fluorescence interference through hardware, the result is not true, and the equipment needs to be increased. The disadvantages of cost and complexity, increased measurement time and difficulty of signal acquisition and processing

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