Method for judging fluorescence quenching degree of Raman spectrum through information entropy

A Raman spectroscopy, fluorescence elimination technology, applied in Raman scattering, material excitation analysis, etc., can solve the problems of unreal results, instrument design limitations, increased instrument cost and complexity, etc., to achieve elimination methods, simple equipment, increased The effect of equipment cost

Active Publication Date: 2016-06-22
OPTOTRACE SUZHOU TECH
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a method for judging the degree of fluorescence elimination of Raman spectrum through information entropy, so as to solve the existing problems of increasing instrument cost and complexity, untrue results, and constituting restrictions on instrument design. Inadequacies

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  • Method for judging fluorescence quenching degree of Raman spectrum through information entropy
  • Method for judging fluorescence quenching degree of Raman spectrum through information entropy
  • Method for judging fluorescence quenching degree of Raman spectrum through information entropy

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Embodiment 1

[0049] A method for judging the degree of fluorescence elimination of sucrose Raman spectrum by information entropy, the method comprises the following steps:

[0050] A. Use Raman laser probe 785nm laser excitation, measure sucrose Raman spectrum, fix focus, set integration time 10s, measure twice with time interval of 30s, record the obtained mixed signal S 1、 S 2 ,like figure 1 shown in, S 1 is the measured spectrum at time 1, S 2 is the measurement spectrum at time 2;

[0051] B. The weight value of the spectrum at the adjustment time 2 is 1.028, which is subtracted from the spectrum at the previous time to obtain the fluorescence decrease metric value ΔS that effectively eliminates the Raman component in it. 3 Spectral S with unweighted direct subtraction 4 for example figure 2 shown, from figure 2 It can be seen that the use of weighted subtraction can effectively eliminate the Raman component;

[0052] C. Select the Savitzky-Golay filter to denoise the fluores...

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Abstract

The invention discloses a method for judging the fluorescence quenching degree of Raman spectrum through information entropy and relates to a Raman spectrum fluorescence quenching method. The method uses the discrete-sequence information entropy as the criterion of fluorescence deduction, fluorescence is gradually deducted from a mixed signal, and the fluorescence in the mixed signal is completely quenched when the information entropy reaches the minimum. The method has the advantages that the fluorescence quenching degree is judged through the information entropy, the method is scientific and reasonable and unlimited by instrument wave-number range, instrument performance can be brought into full play, and the freedom degree of instrument design and manufacturing is increased; the limitation that the fluorescence residue situation needs to be judged in a specific wavelength range in the prior art is solved, no special requirement on spectrum range is needed, and the method is adaptive to instrument design with different wavelength settings; Raman true signals which conform to actual mechanisms and are not interfered by fluorescence can be obtained fast and accurately while equipment complexity is not increased and an existing measuring habit is not changed, and the method is easy to popularize and use.

Description

technical field [0001] The invention relates to a Raman spectrum fluorescence elimination method, in particular to a method for judging the fluorescence elimination degree of Raman spectrum by information entropy. Background technique [0002] Laser Raman spectroscopy can provide molecular spectral information, and at the same time has the advantages of being less susceptible to moisture interference, non-contact and non-destructive to the sample, and is a rapidly developing qualitative and quantitative analysis method. However, the fluorescence generated at the same time as the excitation of Raman scattering interferes with the distinguishability of the Raman spectrum and the quality of the signal. [0003] The micro-spectroscopic Raman spectrometer using array photosensitive has developed rapidly in recent years. The advantages are simple structure and no complicated maintenance. However, the use of hardware to eliminate fluorescence interference will increase the complexi...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 姚志湘粟晖刘柳
Owner OPTOTRACE SUZHOU TECH
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