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Production of oligosaccharides

An oligosaccharide, product technology, applied in the application field of hydrolase in this type of method, can solve the problems of expensive technology, time-consuming and the like

Pending Publication Date: 2016-06-29
CHR HANSEN HMO GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Even though small-scale synthesis of desired oligosaccharides is possible, developing synthetic routes is often time-consuming, technically challenging and often prohibitively expensive

Method used

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  • Production of oligosaccharides
  • Production of oligosaccharides
  • Production of oligosaccharides

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Embodiment Construction

[0088] β-galactosidase was added to a solution containing fermentation-derived lacto-N-tetraose and lactose (both at 10 mM concentration); 50 units / ml (final concentration) of β-galactosidase (Sigma-Aldrich Chemie GmbH, Munich Germany, catalog number G6008)) to decompose lactose in glucose and galactose for better chromatographic separation of sugars (eg by size-dependent gel filtration chromatography). It can be shown that within a few seconds lactose is broken down into its monosaccharides galactose and glucose, while lacto-N-tetraose remains the same (see also figure 1 ). These experiments demonstrate the highly selective action of purified E. coli β-galactosidase, which selectively hydrolyzes only the Gal(β1-4)Glc glycosidic linkages and not the non-reducing ends present in lacto-N-tetraose Gal(β1-3)GlcNAc glycosidic bond at the place. therefore, figure 1 A shows the overlaid HPLC chromatograms of 10 mM lactose and 10 mM lacto-N-tetraose (authentic standard), and figur...

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Abstract

The present invention relates to the use of one or more glycosidases in the process for the production and / or purification of a produced desired oligosaccharide. The process is preferably a microbial fermentation process using a host microorganism, which may also comprise nucleic acids expressing sugar catabolic pathway proteins suitable for the degradation of saccharides otherwise hindering the purification of the desired oligosaccharide.

Description

technical field [0001] The present invention relates generally to methods for the production of oligo- or polysaccharides, such as, for example, via microbial fermentation, and in particular to the use of hydrolases in such methods. Background technique [0002] The commercial production of oligosaccharides, along with the development of novel biomanufacturing techniques, has become of increasing interest over the past few years. Today, oligosaccharides are used, for example, as functional food components, nutritional supplements or as nutraceuticals (nutraceuticals). Traditionally, oligosaccharides are defined as polymers of 2 or more (usually up to 10) monosaccharides, however, in addition polymers of up to 20 to 25 monosaccharides are often assimilated with them. In particular, prebiotic oligosaccharides (prebiotic oligosaccharides) are of high interest because they represent noncariogenic and nondigestible compounds that stimulate the human gastrointestinal microflora (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/26C07H1/06
CPCC12P19/14C12Y302/01C07H1/00C12P19/26C12P19/00C12N15/63C12P19/20C12N1/205C12R2001/19C12N15/72C12N2800/101C12Y302/01023C07H1/06C12P19/12
Inventor 斯特凡·延内魏因
Owner CHR HANSEN HMO GMBH
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