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Plant root secreta generation and collection device and collection method thereof

A technology of root exudates and collection devices, which is applied in the direction of sampling devices, etc., and can solve the problems of perishable roots, inability to continuously sample, and easy damage to samples

Active Publication Date: 2016-09-28
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a device and method for monitoring root exudates changes at any time without disturbing the growth of the root system, without destroying the rhizosphere environment, and at any time after the infection of the rhizosphere pathogens. Susceptible to microbial contamination, sampling is easy to damage the root system, and continuous sampling is not possible. Provide a method for collecting plant root exudates that can be continuously sampled during soil stress under natural conditions.

Method used

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  • Plant root secreta generation and collection device and collection method thereof

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Embodiment Construction

[0022] The invention provides a device for generating and collecting plant root exudates, which includes a plant growth device, a root growth guide device and a root eluting and exudate collection device, and the plant growth device is filled with nutrient soil for planting and planting of the plant to be tested. Nutrient supply.

[0023] The plant growth period is located above the root growth guiding device, and an secretion collecting device is installed below the root growth guiding device.

[0024] Described root growth guiding device is made up of mesh structure and plastic partition, and the aperture size of described mesh structure is The plastic partition is a plastic partition matching the bottom of the plant grower.

[0025] After the plant grower is connected with the root growth guiding device, when collecting plant root exudates, the net structure is 0.5cm thick agar; Bacteria 0.5cm thick medium (oat, PDA, or NA, etc.).

[0026] The nutrient soil needs to be ...

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Abstract

The invention provides a plant root secreta generation and collection device and a collection method thereof. The device comprises a plant growth device, a root growth guiding device and a root elution and secreta collection device. The root growth guiding device comprises a net structure and a plastic separator plate. The collection method is a plant root secreta collection method under soil stress under natural conditions and does not interfere root growth and damage a rhizosphere environment. The collection method comprises root secreta effective expression without outside interference after single pathogen infection and monitoring of root secreta change after rhizosphere pathogen infection.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to a device for generating and collecting plant root exudates and a collecting method thereof. Background technique [0002] In the process of plant growth and development, the root system, as the contact part of the plant and the soil, not only absorbs water and nutrients from the soil, but also releases some inorganic ions and organic compounds around the root through root secretion, these substances are collectively called root exudates. A large number of studies have shown that root exudates affect soil biological environmental changes and allelopathy, and different crops have different effects on soil microbial communities. Crops play an important role in the soil microbial community through their root exudates. Different crops have different component contents in root exudates, and the same crops have different component contents in root exudates after being infected ...

Claims

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Application Information

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IPC IPC(8): G01N1/02
CPCG01N1/02
Inventor 冯超陈德鑫王凤龙张成省王静王文静田雪莹
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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