Sample quantitation with a miniature mass spectrometer

Abstract

The invention generally relates to sample analysis with a miniature mass spectrometer. In certain embodiments, the invention provides methods that involve generating ions of a first analyte and ions of a second analyte. Those ions are transferred through a discontinuous sample introduction interface into a first ion trap of a mass spectrometer in a manner in which the discontinuous sample introduction interface remains open during the transferring. The discontinuous sample introduction interface is closed and the ions are sequentially transferred to a second ion trap of the mass spectrometer where they are sequentially analyzed.

Description

[0001] Related applications [0002] This application claims the benefit and priority of U.S. Provisional Application Serial No. 62 / 013,005, filed June 17, 2014, and U.S. Provisional Application Serial No. 61 / 865,377, filed August 13, 2013, the contents of which Incorporated herein by reference in its entirety. [0003] governmental support [0004] This invention was made with government support under GM106016 awarded by the National Institutes of Health. The government has certain rights in this invention. technical field [0005] The present invention generally relates to sample quantification using a miniature mass spectrometer. Background technique [0006] In commercial HPLC-MS systems, triple quadrupole mass spectrometers are typically used to measure the relative intensities of characteristic fragment peaks of analytes and their internal standards for quantification (MRM, Multiple Reaction Monitoring). However, triple quadrupole mass spectrometers are large be...

AI Technical Summary

Problems solved by technology

For miniature mass spectrometers equipped with discrete sample introduction interfaces, measurements of analyte and internal standard intensities are performed with a minimum time difference of one second, which leads to inaccuracies in quantification

Images