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Coagulase detection reagent, reaction pad, preparation method and kit thereof

A technology for detecting reagents and coagulase, which is applied in biochemical equipment and methods, measuring devices, and microbial determination/inspection, etc. Effect

Active Publication Date: 2018-10-09
GUANGZHOU HONGQI OPTICAL INSTR TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the accuracy of the detection reagents currently used to detect coagulase is lacking

Method used

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  • Coagulase detection reagent, reaction pad, preparation method and kit thereof
  • Coagulase detection reagent, reaction pad, preparation method and kit thereof
  • Coagulase detection reagent, reaction pad, preparation method and kit thereof

Examples

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preparation example Construction

[0056] The preparation method of this reaction pad comprises the following steps:

[0057] 1) Prepare materials: prepare morpholineethanesulfonic acid, sucrose, polyvinylpyrrolidone and glycyl-arginyl tetramethoxy-β-naphthylamine according to the above parts by weight;

[0058] 2) Configure morpholineethanesulfonic acid buffer solution (MES): dissolve morpholineethanesulfonic acid in a water solvent to obtain morpholineethanesulfonic acid buffer solution for use;

[0059] 3) Prepare soaking solution: dissolve sucrose, polyvinylpyrrolidone and glycyl-arginyl tetramethoxy-β-naphthylamine in morpholine ethanesulfonic acid buffer to obtain soaking solution;

[0060] 4) Soaking liquid treatment: put the filter paper in the soaking liquid and fully soak it for 10-20 seconds, take it out, and then dry it at a temperature of 20-70° C. for 30-40 minutes to obtain a reaction pad.

[0061] More specifically, the filter paper may be Whatman glass fiber filter paper, GF / A Whatman fiber fi...

Embodiment 1

[0067] Embodiments 1 to 3 disclose coagulase detection reagents, respectively prepare three groups of raw material components according to Table 1, and prepare morpholineethanesulfonic acid buffer solutions with different concentrations according to Table 2; prepare chromogen raw materials according to Table 3; Group A is used for Example 1, Group B is used in Example 2, and Group C is used in Example 3.

[0068] Sucrose, polyvinylpyrrolidone and glycyl-arginyl tetramethoxy-β-naphthylamine were successively dissolved in 100 mL of morpholinoethanesulfonic acid buffer to obtain a reactant.

[0069] Dissolving cinnamaldehyde in 10 mL of hydrochloric acid solution to obtain a chromogenic agent, and combining the chromogenic agent with a reactant to obtain a coagulase detection reagent.

[0070] The composition and content of table 1 reactant

[0071]

[0072] The preparation parameters of table 2 morpholineethanesulfonic acid buffer

[0073]

[0074] The composition and co...

Embodiment 4～6

[0083] Embodiment 4~6 discloses a kind of kit:

[0084] 1) according to table 1, table 2 and table 3, prepare materials and prepare morpholine ethanesulfonic acid buffer; A group is used for embodiment 4, and B group is used for embodiment 5, and C group is used for embodiment 6;

[0085] 2) Prepare soaking solution: dissolve sucrose, polyvinylpyrrolidone and glycyl-arginyl tetramethoxy-β-naphthylamine in morpholine ethanesulfonic acid buffer to obtain soaking solution;

[0086] 3) Soaking solution treatment: put the filter paper in the soaking solution and fully soak it for 10-20 seconds, then take it out, and then dry it at a temperature of 40°C for 30-40 minutes to obtain a reaction pad;

[0087] 4) Place the reaction pad: place the reaction pad in the reaction well, and then seal the reaction well;

[0088] 5) Preparation of a color developer: dissolving cinnamaldehyde in hydrochloric acid solution to obtain a color developer; combining the color developer with a reaction...

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Abstract

The invention discloses a coagulase detection reagent, a reaction pad, a preparation method thereof and a kit. The coagulase detection reagent comprises reaction agents and a color developing agent, the reaction agents include morpholine ethane sulfonic acid, cane sugar, polyvinylpyrrolidone and glycyl-protamine tetramethoxy-beta-naphthylamine, the reaction pad comprises a carrier and ingredients of the reaction agents, and the ingredients of the reaction agents are coated on the carrier. The preparation method of the reaction pad includes: 1), dissolving the cane sugar, polyvinylpyrrolidone and glycyl-protamine tetramethoxy-beta-naphthylamine in morpholine ethane sulfonic acid to obtain soaking liquid; 2), putting the carrier in the soaking liquid, taking out after sufficient soaking, and drying to obtain the reaction pad. The reaction pad obtained by the preparation method is combined with ingredients of the detection agent to be applied in preparing the kit which is high in sensitivity and accuracy.

Description

technical field [0001] The invention relates to the field of detection reagents for aerobic bacterial vaginitis, in particular to a coagulation enzyme detection reagent, a reaction pad, a preparation method thereof and a kit. Background technique [0002] Vaginitis is an inflammation of the vaginal mucosa and submucosal connective tissue, and is a common disease in gynecological clinics. For normal healthy women, due to anatomical and biochemical characteristics, the vagina has a natural defense function against the invasion of pathogens. When the natural defense function of the vagina is destroyed, pathogens are easy to invade and cause vaginal inflammation. There are three types of common vaginitis: bacterial vaginosis (BV / AV), fungal vaginitis (VVC) and trichomonas vaginitis (TV). Among them, female bacterial vaginal infection can be divided into two types, one is caused by anaerobic bacteria and facultative anaerobic bacteria, clinically called bacterial vaginosis (BV),...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/56
CPCG01N33/573
Inventor 眭红燕朱华琳范静彦李必松
Owner GUANGZHOU HONGQI OPTICAL INSTR TECH
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