Application of a ginseng-derived nanoparticle in the preparation of a drug for treating tumors
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A technology of nanoparticles and ginseng, which is applied in the field of anti-tumor drugs, can solve the problem that new drugs with anti-tumor functions have not been effectively developed, and achieve the effects of improving tumor microenvironment, easy operation and less time-consuming
Active Publication Date: 2021-04-27
JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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The development of new drugs with anti-tumor function based on ginseng has not been effectively developed
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Embodiment 1
[0041] Example 1: Preparation of ginseng-derived nanoparticles
[0042] (1) Wash fresh ginseng with clean water, and extract the original pulp through low-speed screw extrusion technology in an environment of 20°C;
[0043] (2) filtering the squeezed raw pulp through a 500-mesh sieve in an environment of 20°C to remove impurities, and collecting the filtrate;
[0044] (3) Centrifuge the collected filtrate at 200×g for 10 minutes, discard the precipitate, and collect the supernatant; then centrifuge the supernatant at 2000×g for 30 minutes, discard the precipitate, and collect the supernatant; Centrifuge at 10,000×g for 60 minutes, discard the precipitate, and collect the supernatant; finally, centrifuge the supernatant at 120,000×g for 60 minutes, and collect the precipitate;
[0045] (4) After the precipitate was resuspended with pH 7.2 phosphate buffer, centrifuge at 120,000×g for 60 minutes to collect the precipitate; , centrifuged for 60 minutes, and the supernatant was ...
Embodiment 2
[0046] Embodiment 2: Observation of the morphology of GDNPs by electron microscope
[0047] The extracted GDNPs were ultracentrifuged at 120,000×g for 60 minutes to precipitate and compress tightly. The supernatant was discarded, the precipitate was fixed with 2.5% (v / v) glutaraldehyde, and sent to the electron microscope room for processing, and the morphology of GDNPs was observed on the microscope . Such as figure 1 Shown: Under the electron microscope, it can be seen that GDNPs are nanoparticles with a membrane structure, and the size is between 150-500nm, which confirms the effective recruitment of nano-scale microbodies derived from ginseng.
Embodiment 3
[0048] Example 3: Particle size and distribution of ginseng-derived nanoparticles analyzed by Malvern particle size analyzer
[0049] The extracted GDNPs were tested for particle size by Malvern particle sizer, such as figure 2 As shown, the peak uniformity of GDNPs is better.
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Abstract
The invention discloses the application of nano particles derived from ginseng in the preparation of drugs for treating tumors. The ginseng-derived nanoparticles have a membrane structure, the particle diameter range is 150-500nm, and the peak particle diameter is 280-350nm; the ginseng-derived nanoparticles are prepared by multiple centrifugation and filtration to remove impurities; the ginseng-derived nanoparticles The particles can effectively depolarize the tumor-associated macrophages (Tumor‑associated macrophages, TAM) in the tumor microenvironment, making them shift from M2 macrophages that promote tumor invasion and metastasis to M1 macrophages that kill tumors. to improve the tumor microenvironment and kill tumors. It has a good application prospect in the development of drugs for the preparation of natural anti-tumor drugs.
Description
technical field [0001] The invention belongs to the field of antitumor drugs, and in particular relates to the application of nano particles derived from ginseng in the preparation of drugs for treating tumors. Background technique [0002] The tumor microenvironment (tumor microenvironment) refers to the tumor cells, stromal cells (including fibroblasts, immune and inflammatory cells, adipocytes, glial cells, smooth muscle cells, and some vascular endothelial cells, etc.) The local homeostatic environment composed of tumor cells and extracellular matrix provides the necessary material basis for the occurrence, development, invasion, and metastasis of tumors. The tumor microenvironment plays an important role in supporting and promoting tumors. How to intervene and improve the tumor microenvironment is the core issue in preventing tumor development and metastasis. [0003] Studies in recent years have shown that the macrophages growing in the tumor microenvironment—Tumor-a...
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Patent Type & AuthorityPatents(China)
IPC IPC(8): A61K9/14A61K36/258A61P35/00
CPCA61K9/14A61K36/258A61K2236/31
Inventor曹萌曹鹏颜怀江
OwnerJIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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