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Cervical cancer susceptibility gene typing detection kit and application thereof

A technology of susceptibility genes and kits, applied in the field of testing, can solve problems such as unknown specific mechanisms, cell inflammation and necrosis, and achieve the effect of predicting the risk of disease in future generations

Inactive Publication Date: 2017-07-25
SHANGHAI YIRUN BIOLOGICAL TECH
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Problems solved by technology

The gene at this site encodes the GSDMB protein, which belongs to the Gasdermin family, which includes GSDMA, GSDMB, GSDMC, DFNA5, DFNB59 and other members. These proteins can lead to cell inflammation and necrosis after receiving the order of the human natural immune system, but The exact mechanism is unknown

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  • Cervical cancer susceptibility gene typing detection kit and application thereof
  • Cervical cancer susceptibility gene typing detection kit and application thereof
  • Cervical cancer susceptibility gene typing detection kit and application thereof

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Embodiment Construction

[0026] The technical solution of this patent will be further described in detail below in conjunction with specific embodiments.

[0027] A cervical cancer susceptibility gene detection and typing kit, the kit includes a DNA extraction kit, a DNA amplification kit and a sequencing kit; the DNA extraction kit adopts the DP322 type of Tiangen Biochemical Technology Co., Ltd. Product, the DNA amplification kit mainly includes amplification primers, enzymes, PCR Buffer, dNTP Mixture, amplification primers, the amplification primers are: 1) F: 5'-TCAGTCCCACCTCAGCA-3' (SEQ ID NO. 1), R: 5'-CAGAAGAAGGGCAAAGC-3' (SEQ ID NO.2), 2) F: 5'-CCCACAACCCATGCATCTGATA-3' (SEQ ID NO.3), R: 5'-GGCCACTGACATCTGCACAAAC-3' (SEQ ID NO.4), 3) F: 5'-GCCCACCTGAGAAAAGCTGAAC-3' (SEQ ID NO.5), R: 5'-AGGCATGTGGGAGCCTGTG-3' (SEQ ID NO.6); the enzyme used Bao Biological Engineering Co., Ltd. TaKaRa Taq TM enzyme; the sequencing kit adopts the Terminator v3.1 Cycle Sequencing Kit.

[0028] The amplificatio...

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Abstract

The invention discloses a cervical cancer susceptibility gene typing detection kit and an application thereof. With the application of the cervical cancer susceptibility gene typing detection kit, polymorphic sites of genes HLA-DPB2, GSDMB / GSDML and HLA-DRB1, related to cervical cancer, are detected, so that genetic factors of individuals in the aspect of the cervical cancer can be understood and relative attack risks of the cervical cancer can be assessed; therefore, the kit has positive significance for early diagnosis, early treatment and early intervention of the cervical cancer. Meanwhile, the kit can also assist diagnosis of the cervical cancer, guide reasonable use of drugs, bring about benefits for predicting a risk of suffering from the disease (the cervical cancer) in offspring and the like.

Description

technical field [0001] The invention relates to the technical field of testing, in particular to a detection and typing kit for cervical cancer susceptibility genes and its application. Background technique [0002] With the completion of the Human Genome Project (HGP), the discovery of a large number of disease-related genes has promoted the transformation of the traditional biomedical model into a predictable, preventable, individualized and participatory genomic medicine model, which will provide a better way for the future. The development of prevention, diagnosis, and treatment of major and complex diseases such as cancer, cardiovascular and cerebrovascular diseases, diabetes, neurological and mental diseases, which have long plagued human beings, has opened up new ways and also provided good opportunities for the industrialization of genetic science. [0003] Genome Wide Association Studies (GWAS) is a case-control association analysis using millions of single nucleoti...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q1/6886C12Q2600/106C12Q2600/118C12Q2600/156C12Q2531/113C12Q2535/101
Inventor 何侃栗娟王佳其他发明人请求不公开姓名
Owner SHANGHAI YIRUN BIOLOGICAL TECH
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