Biomarker for diagnosis of hepatoma and use thereof

A technology of biomarkers and markers, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low-accuracy screening and inspection, and achieve the effect of reducing medical expenses

Active Publication Date: 2017-08-29
SEOUL NAT UNIV R&DB FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the individual serum markers are significant as prognostic factors, but have not been used in screening tests due to low accuracy when used alone, and the method of early diagnosis of liver cancer using a single serological marker is considered to have reached its limit
In addition, patients who are actually diagnosed at a stage where radical treatment such as surgery or high-frequency thermotherapy can be performed are limited to about 30% of all liver cancer patients.

Method used

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  • Biomarker for diagnosis of hepatoma and use thereof
  • Biomarker for diagnosis of hepatoma and use thereof
  • Biomarker for diagnosis of hepatoma and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Embodiment 1: Clinical sample information used in the present invention

[0094] This experiment was carried out in accordance with the protocol approved by the Seoul National University Medical Research Ethics Review Committee, and written consent based on sufficient explanation was obtained from each patient, and the clinical information is shown below.

[0095] In order to select a marker candidate group and to select a protein candidate group showing expression differences from pooling samples of normal people and liver cancer patients, 60 normal people (male / female=41 / 19) and 60 liver cancer patients were used. A sample of people (male / female=42 / 18) was studied. In fact, in order to apply and analyze individual samples, 30 patients with liver cirrhosis, 30 patients with liver cancer before treatment, and 30 patients who recovered from liver cancer after treatment were used in the primary sample group, and 50 patients with liver cirrhosis were used in the secondary ...

Embodiment 2

[0096] Embodiment 2: Data Mining for Selecting Target Candidate Groups

[0097] Target candidate populations were selected as follows. A candidate group of protein markers for early diagnosis of liver cancer is secured using the Liver Atlas database, which is the most comprehensive resource currently associated with liver diseases. There are a total of 50,265 proteins known to be associated with liver diseases in the Liver Atlas database. In order to select only the proteins that can be detected from the blood, only the proteins that can be secreted are screened. A total of 1,683 proteins were selected as a result of proteins in blood or possibly secreted (Uniprot database (protein database) standard). Finally, in order to select only the proteins that can be detected by the mass analyzer, four different peptide MS / MS library sources (NIST Ion-Trap, NIST Q-TOF, ISB human plasma (human plasma), Home made library (domestic library)) to select only proteins with peptide MS / MS d...

Embodiment 3

[0098] Example 3: Derivation of target candidate populations by MRM analysis

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Abstract

Disclosed in the present application is a marker or a maker combination enabling diagnosis, prognosis, or treatment monitoring of hepatoma with high accuracy and sensitivity. The marker according to the present application enables simple and effective diagnosis or monitoring of hepatoma by a noninvasive test using blood, thus can be developed into diverse test kits, and can be very usefully utilized in early detection of hepatoma at home or in general practice, for example, through POCT development.

Description

technical field [0001] The invention relates to a biomarker for liver cancer diagnosis and its application. Background technique [0002] Liver cancer or hepatocellular carcinoma (Hepatocellular carcinoma, HCC) is the most common type of liver cancer in adults and occupies the third place in the cause of death due to cancer (Stefaniuk P, et al., 2010, World JGastroenterol 16:418- 424 (Stephaniek P et al., 2010, World Journal of Gastroenterology 16:418-424)). Liver cancer is a disease that does not show symptoms until it has advanced to a certain extent, so the appropriate treatment period is often missed, and the prognosis is very poor even if treatment is given. In particular, liver cancer is a critical disease that can cause death within one year when surgical resection is not possible. Considering the clinical reality, technologies that enable early cancer diagnosis and prognosis prediction are the most realistic measures for cancer treatment , and a guideline for a new...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/533
CPCG01N33/533G01N33/57438G01N33/574
Inventor 金永洙尹晶焕金炫秀
Owner SEOUL NAT UNIV R&DB FOUND
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