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OPN (osteopontin), GDF15 (growth differentiation factor-15), NSE (neuron-specific enolase), TRAP5 (tartrate resistant acid phosphatase 5) and OPG (osteoprotegerin) combined diagnosis kit

A kit and reagent technology, applied in disease diagnosis, biological testing, material testing products, etc., can solve the problem that sensitivity and specificity cannot meet the requirements of clinical diagnosis, and achieve short cycle time, high sensitivity and good specificity. Effect

Active Publication Date: 2017-12-12
SHANGHAI CHANGHAI HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alpha-fetoprotein (AFP) is the preferred serum tumor marker for the diagnosis of liver cancer. It has been widely used in high-risk population screening, diagnosis, and prognosis evaluation. Sensitivity and specificity are far from meeting the requirements of clinical diagnosis. Therefore, an in-depth study of serum tumor markers for early liver cancer is needed.

Method used

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  • OPN (osteopontin), GDF15 (growth differentiation factor-15), NSE (neuron-specific enolase), TRAP5 (tartrate resistant acid phosphatase 5) and OPG (osteoprotegerin) combined diagnosis kit
  • OPN (osteopontin), GDF15 (growth differentiation factor-15), NSE (neuron-specific enolase), TRAP5 (tartrate resistant acid phosphatase 5) and OPG (osteoprotegerin) combined diagnosis kit
  • OPN (osteopontin), GDF15 (growth differentiation factor-15), NSE (neuron-specific enolase), TRAP5 (tartrate resistant acid phosphatase 5) and OPG (osteoprotegerin) combined diagnosis kit

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Experimental program
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Effect test

Embodiment 1

[0049] 1. Preparation of detection kit

[0050] The detection kit is prepared according to the following composition, and the kit is suitable for detecting the expression of OPN, GDF15, NSE, TRAP5 and OPG in plasma of biological samples by a liquid-phase protein chip method:

[0051] (a) OPN, GDF15, NSE, TRAP5 and OPG detection antibody Bead

[0052] (b) Experimental standard product and quality control product 1 and quality control product 2

[0053] (c) Serum or plasma matrix, Assay Buffer, Wash Buffer, Bead diluent

[0054] (d) Detection antibody, phycoerythrin streptavidin, 96-well plate corresponding to the experiment

[0055] (e) Description of the experimental method

[0056] 2. Relationship between OPN / GDF15 / NSE / TRAP5 / OPG expression and early liver cancer

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Abstract

The invention relates to the field of diagnosis kits, particularly to application of OPN, GDF15, NSE, TRAP5 and OPG to preparation of early liver cancer diagnosis detecting reagents or kits. The invention also discloses a kit which is used for diagnosing early liver cancer in a combined mode by detecting content of OPN, GDF15, NSE, TRAP and OPG in plasma. The kit has the advantages of being high in specificity and sensitivity, can be applied to early diagnosis of liver cancer, therapeutic effect evaluation during treatment and post-treatment metastasis and recurrence monitoring and can provide detecting results earlier than clinical symptoms. The kit and detecting technology is mature, short in cycle and good in specificity.

Description

technical field [0001] The present invention relates to the technical field of diagnostic kits, specifically, a kit for the combined diagnosis of early liver cancer by detecting the contents of OPN, GDF15, NSE, TRAP5 and OPG in plasma, and provides a new method and kit for the early diagnosis of liver cancer . Background technique [0002] Liver cancer refers to malignant tumors that occur in the liver and can be divided into two categories: primary and secondary. Primary liver cancer is the sixth most common cancer in the world, with 782,500 new cases and 745,500 deaths every year. It is also the third most common malignant tumor in mortality after gastric cancer and esophageal cancer. There are clear geographic differences in its incidence, with the vast majority of liver cancer cases occurring in developing countries, but rates are also increasing in North America and Europe. The high heterogeneity of HCC makes it difficult to eradicate cancer cells with chemotherapy al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/573G01N33/68
CPCG01N33/573G01N33/574G01N33/57438G01N33/57484G01N33/6863G01N33/6893G01N2333/4746G01N2333/4753G01N2800/085
Inventor 刘善荣程树群程凯刘子鑫石洁贾音张慧万思琴余喜亚
Owner SHANGHAI CHANGHAI HOSPITAL
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