Protective solution for earthworm egg cocoon storage
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A technology for earthworm eggs and protective liquid, which can be used in applications, preservation of human or animal bodies, animal husbandry, etc., and can solve problems such as inconvenience
Active Publication Date: 2017-12-22
YANTAI YULONG BIOTECH CO LTD
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[0003]
In summary, there are obviously inconveniences and defects in
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Embodiment 1
[0034] A, prepare phosphate buffer saline (PBS), pH value: 7.2;
[0035] B. Add glycerol: 10%, polysucrose: 5%, PVP: 10% to the PBS buffer in step A and mix well;
[0036] C, use the protective solution obtained in step B to process the cocoons of earthworm eggs and store after drying;
[0037] D. Take out the earthworm egg cocoons treated in step C after being preserved for 12 months, disperse them in the soil, and give appropriate conditions, hatching rate: 95%;
Embodiment 2
[0039] A, prepare phosphate buffer saline (PBS), pH value: 7.2;
[0040] B. Add glycerol: 5%, trehalose: 5%, PVP: 10% to the PBS buffer in step A and mix well;
[0041] C, use the protective solution obtained in step B to process the cocoons of earthworm eggs and store after drying;
[0042] D. Take out the earthworm egg cocoons treated in step C after being preserved for 12 months, disperse them in the soil, and give appropriate conditions, hatching rate: 95%;
Embodiment 3
[0044] A, prepare phosphate buffer saline (PBS), pH value: 7.0;
[0045] B. Add glycerol: 5%, polysucrose: 5%, PVP: 5% to the PBS buffer in step A and mix well;
[0046] C, use the protective solution obtained in step B to process the cocoons of earthworm eggs and store after drying;
[0047] D. Take out the earthworm egg cocoons treated in step C after being stored for 9 months, disperse them in the soil, and give appropriate conditions, hatching rate: 85%;
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Abstract
The invention relates to the technical field of earthworm egg cocoon storage, in particular to a protective solution for earthworm egg cocoon storage. The protective solution is prepared through the following steps: A, a phosphate buffer with PH being 6.8-7.6 is prepared; B, 5%-15% of glycerin, 5%-15% of polysucrose and 0-30% of PVP (polyvinylpyrrolidone) are added to the phosphate buffer in the step A, the phosphate buffer is taken as a standard for the adding amounts of the three components, glycerin is added by volume, and polysucrose and PVP are added by massic volume. Therefore, earthworm egg cocoons are soaked in the protective solution which is prepared from the phosphate buffer by adding glycerin, polysucrose and PVP in specific concentration to the phosphate buffer and uniformly mixing the components, or the protective solution is sprayed to the surfaces of the earthworm egg cocoons, and the earthworm egg cocoons can be dehydrated slowly and stored in a low-temperature environment for a long time. When placed in an environment with proper conditions, the earthworm egg cocoons can hatch rapidly and the hatching rate is high. The protective solution is applicable to fields of earthworm egg cocoon storage and transportation, bio-fertilizer, soil improvement and the like.
Description
technical field [0001] The invention relates to the technical field of earthworm egg cocoon storage, in particular to a protective solution for earthworm egg cocoon storage. Background technique [0002] Earthworms are hermaphroditic animals, and their eggs are wrapped in light yellow-green oocysts (also known as egg cocoons), and about a dozen eggs are wrapped in one oocyst. These oocysts will hatch into small earthworms under suitable environmental conditions. At present, the conventional practice in earthworm culture is to separate earthworm eggs from adult earthworms and continue hatching culture, or to store them at low temperature after separation. There is no report about the further preservation and application of earthworm egg cocoons. If special technical means are used to protect the cocoons, the cocoons of earthworm eggs can be preserved for a long time, and a high hatching rate can be guaranteed. Therefore, the earthworm egg cocoon will be more convenient for ...
Claims
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