A kind of preparation method of bikokoline base and protopine

Abstract

The invention discloses a preparation method of bicuculline and fumarine. The preparation method comprises the following steps: 1) extracting: pulverizing a medicinal material, extracting with an acidmethanol or ethyl alcohol solution to obtain an extracting solution A, and conducting vacuum concentration to obtain a concentrated solution B; 2) separating and enriching: taking the concentrated solution B, regulating the PH, adsorbing through an inverse polymerization filler C, eluting with water firstly to obtain a washing solution D, then desorbing with an alcoholic solution E, collecting adesorbed solution F, and desorbing with an alcoholic solution G to obtain a desorbed solution H; 3) purifying: taking the desorbed solution H, conducting vacuum concentration to regulate the PH, standing, filtering a precipitate, stirring and mixing the precipitate, obtaining a solution J through silica gel chromatograph; conducting vacuum concentration on the desorbed solution F, regulating the PH to 9-10, and separating out a precipitate K; and regulating the PH of the washing solution D to 9-10, and separating out a precipitate L; and 4) crystalizing and drying: conducting vacuum concentration on the solution J, crystalizing and drying the solution J to obtain a pure product of bicuculline; and crystalizing and drying the precipitate K and the precipitate L to obtain a pure product of fumarine. By using the method, the yield is high, the purity is high, and the method can be applied to industrial production.

Description

technical field

[0001] The invention relates to the field of natural medicinal chemicals, in particular to a method for preparing bikokolinine and protopine. Background technique

[0002] Bicuculline (+)-Bicuculline molecular formula is C 20 h 17 NO 6 , molecular weight 367.36, CAS No. 485-49-4, light yellow powder, soluble in benzene, chloroform, ethyl acetate, slightly soluble in ethanol and ether; the molecular formula of Protopine is C 20 h 19 NO 5 , Molecular weight 353.37, CAS No. 130-86-9, white powder, soluble in benzene, chloroform, ethyl acetate, slightly soluble in ethanol and ether, these two compounds are alkaloid substances, and their structural formula is as follows.

[0003]

[0004] In the current technology, there is no preparation method that can separate and purify bikokolinine from plants, and the extraction method for the original opioidine also has the problems of low yield and difficulty in industrial production. Contents of the invention ...

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