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Sowbug symbiotic bacterium of chaetomium globosum and application thereof to preparation of antitumor compounds

A technology of Chaetomium globosa and its anti-tumor effect, which is applied in the field of medicine and can solve the problem of very little research on symbiotic bacteria and their metabolites

Inactive Publication Date: 2018-02-13
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with the species diversity of the mulewort, there are very few studies on its symbionts and their metabolites

Method used

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  • Sowbug symbiotic bacterium of chaetomium globosum and application thereof to preparation of antitumor compounds
  • Sowbug symbiotic bacterium of chaetomium globosum and application thereof to preparation of antitumor compounds
  • Sowbug symbiotic bacterium of chaetomium globosum and application thereof to preparation of antitumor compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Preparation and structure identification of the compound chaetomin A.

[0035] (1) Preparation of compound chaetomycin A shown in formula one

[0036] 1. Fermentation conditions

[0037] Preparation of seed culture solution: Take 3.0 grams of yeast extract, 3.0 grams of malt extract, 5.0 grams of peptone, and 10.0 grams of glucose, dissolve them in an appropriate amount of water, then dilute to 1 liter with water, sterilize at 121°C for 30 minutes, and set aside. Inoculate the symbiotic fungus Chaetomium globosa (strain preservation number: CCTCC M2016343) into the above-mentioned medium, and culture it on a shaker at 25-28°C and 100-120 rpm for 3-5 days to obtain seed culture liquid.

[0038] Fermentation: put 200 grams of rice into a 1-liter Erlenmeyer flask, add 200 milliliters of water, sterilize at 121°C for 30 minutes, and set aside. The above seed culture solution is inoculated into the rice culture medium, and cultured statically for 25-30 days und...

Embodiment 2

[0046] Example 2: Preparation and structure identification of the compound chaetomin B.

[0047] (1) Preparation of compound chaetomycin B shown in formula 2

[0048] 1. Fermentation conditions

[0049] Preparation of seed culture solution: Take 3.0 grams of yeast extract, 3.0 grams of malt extract, 5.0 grams of peptone, and 10.0 grams of glucose, dissolve them in an appropriate amount of water, then dilute to 1 liter with water, sterilize at 121°C for 30 minutes, and set aside. Inoculate the symbiotic fungus Chaetomium globosa (strain preservation number: CCTCC M2016343) into the above-mentioned medium, and culture it on a shaker at 25-28°C and 100-120 rpm for 3-5 days to obtain seed culture liquid.

[0050] Fermentation: put 200 grams of rice into a 1 liter Erlenmeyer flask, add 200 milliliters of water, sterilize at 121°C for 30 minutes, set aside. The above seed culture solution is inoculated into the rice culture medium, and cultured statically for 25-30 days under the...

Embodiment 3

[0058] Example 3: Preparation and structure identification of the compound chaetomin C.

[0059] (1) Preparation of compound chaetomycin C shown in formula three

[0060] 1. Fermentation conditions

[0061] Preparation of seed culture solution: Take 3.0 grams of yeast extract, 3.0 grams of malt extract, 5.0 grams of peptone, and 10.0 grams of glucose, dissolve them in an appropriate amount of water, then dilute to 1 liter with water, sterilize at 121°C for 30 minutes, and set aside. Inoculate the symbiotic fungus Chaetomium globosa (strain preservation number: CCTCC M2016343) into the above-mentioned medium, and culture it on a shaker at 25-28°C and 100-120 rpm for 3-5 days to obtain seed culture liquid.

[0062] Fermentation: put 200 grams of rice into a 1-liter Erlenmeyer flask, add 200 milliliters of water, sterilize at 121°C for 30 minutes, and set aside. The above seed culture solution is inoculated into the rice culture medium, and cultured statically for 25-30 days u...

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Abstract

The invention belongs to the technical field of medicine, and relates to a sowbug symbiotic bacterium of chaetomium globosum and application thereof to the preparation of indole alkaloid compounds with the antitumor effects. The invention provides activity and structures of new indole alkaloid compounds 1 to 10 from the secondary metabolites of the chaetomium globosum, and provides a separation purification preparation method and structure identification of the new compounds. The chaetomium globosum provided by the invention is preserved in CCTCC (China Center for Type Culture Collection), andhas the strain preservation number being CCCC M2016343. The preservation time is June 23, 2016; the preservation address is Wuhan University, Wuhan, China. The invention provides backup compounds fordeveloping the novel anti-tumor medicine, and has the important significance on the development and utilization of medical insect symbiotic bacterium resources.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a symbiotic bacterium named chaetomium globosum, and its application in the preparation of compounds with anti-tumor effects, as well as the produced compounds with anti-tumor effects and their preparation method. Background technique [0002] Symbiotic bacteria are a large group of special microorganisms that live together with the host for a long time. According to different hosts, they can be divided into plant endophytes, rodent symbiotic bacteria, sponge symbiotic bacteria and other symbiotic bacteria. Symbiotic bacteria are an important source of active secondary metabolites, which also play a very important role in the murine body. With the limitation of plant resources, the probability of discovering new active ingredients from plants and plant endophytes is getting smaller and smaller. There are very rich types of mouseworts, and nearly 1 million species have been repor...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/16C07D491/044C07D491/107C07D209/96A61P35/00C12R1/645
CPCC07D209/96C07D491/044C07D491/107C12P17/16C12N1/145C12R2001/645
Inventor 张勇慧薛永波陈春梅朱虎成张锦文
Owner HUAZHONG UNIV OF SCI & TECH
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