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A microfluidic chip-based glomerular system and its application

A microfluidic chip and glomerular technology, applied in the field of glomerular system, can solve problems such as the inability to reproduce renal filtration function in vitro

Active Publication Date: 2021-03-19
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been a lot of work related to the simple in vitro culture of kidney cells for evaluation, most of which focus on simple cytotoxicity, and the filtration function of the kidney cannot be reproduced in vitro. The construction of a functional in vitro kidney model still has great potential. challenge

Method used

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  • A microfluidic chip-based glomerular system and its application
  • A microfluidic chip-based glomerular system and its application
  • A microfluidic chip-based glomerular system and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Construction and functional characterization of glomerulus chip

[0032] A microfluidic chip, such as figure 2 As shown: the microfluidic chip is composed of left main channel 1, right main channel 4, collagen channel 3, collagen channel entrance 5, left main channel entrance 6, and right main channel entrance 7. Collagen channel 3 is connected to the left Main channel 1 on the left, and main channel 4 on the right.

[0033] The main channel 1 on the left side is I-shaped, the main channel 4 on the right side of the chip is U-shaped, and the collagen channel 3 is in the shape of "Feng", which is a horizontal structure in the middle of the collagen channel, and the collagen channel passes through the The structure is connected with the left main channel 1 and the right main channel 4, and the number of transverse structures is 3; the junction of the collagen channel 3 and the left main channel 1 is a hemispherical interface, and the collagen side is a concave surface. ...

Embodiment 2

[0038] Application in the evaluation of nephrotoxicity

[0039] The glomerulus chip was constructed, cultured by adding cadmium acetate containing 0, 1, 2, 4, 8, and 16 μM cell culture solution, and the main channel culture solution was collected after 24 hours of treatment for LDH leakage detection. The results are as follows: Figure 5as shown in c. It can be seen that as the concentration of cadmium acetate increases, the leakage of LDH in glomeruli increases, and high concentration of cadmium acetate produces obvious nephrotoxicity. Carry out cck-8 quantitative test and life-and-death staining on the glomeruli, and take pictures under the fluorescence microscope. The results are as follows: Figure 5 a and Figure 5 as shown in b. With the increase of cadmium acetate concentration, the dead cells of glomerulus increased, and the cell viability decreased obviously. High concentration of cadmium acetate produced obvious nephrotoxicity. IgG (red fluorescence) was added to...

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Abstract

The invention relates to a glomerulus system based on a micro-fluidic chip and applications of the glomerulus system. The micro-fluidic chip is composed of a left main channel, a right main channel, acollagen channel, a collagen channel inlet, a left main channel inlet and a right main channel inlet, wherein the left main channel is connected to the left side of the collagen channel, and the right main channel is connected to the right side of the collagen channel; the left main channel is inoculated with a glomerulus micro-tissue, the right main channel is used as a collecting area, the filtering effect of the glomeruli can be observed, and the influences of the renal toxicity caused by the chemical ingredients on the filtering function of the glomeruli also can be observed. According tothe technical scheme, the construction of a glomerulus chip with the filtering function outside the body is realized, and the application in the kidney toxicity evaluation of the medicines is also realized.

Description

technical field [0001] The invention relates to the technical field of applying microfluidic chip technology to the construction of in vitro organ models, in particular to a microfluidic chip-based glomerulus system and its application. Background technique [0002] The kidney is one of the main target organs of drug toxicity. The kidney is composed of more than 20 types of cells with different ultrastructure, metabolic capacity and transport function, and is an important organ for drug excretion. When chemical drugs are excreted by the kidney, they will be selectively enriched in kidney cells, causing damage to cell membranes, mitochondria, endoplasmic reticulum and lysosomes, destroying the integrity of cells, and causing apoptosis or necrosis of kidney cells. Reduce the filtration function of glomerulus and the reabsorption function of renal tubules, and then cause the imbalance of water and electrolyte in the body, and even cause acute renal failure in severe cases. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12N5/071C12Q1/02
CPCC12M21/08C12M23/16C12N5/0684C12N2503/02C12N2533/54G01N33/5014G01N2500/10
Inventor 秦建华李中玉陶婷婷郭雅琼
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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