A kind of purple flower trigeminal white and tissue culture rooted seedlings for hardening and transplanting method

A purple flower trident and hardening technology, which is applied in gardening methods, fertilization methods, botanical equipment and methods, etc., can solve the problems of high infection rate of stem rot, poor field planting effect, and low transplanting survival rate, etc. Effects of infecting, improving the survival rate of transplanting, and improving the survival rate

Active Publication Date: 2020-12-11
云南恩润生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

People have begun to try artificial planting of Radix Sinensis, and the existing tubers are used to plant, and plant tissue culture technology is used to propagate Radix Radix and seedlings are planted. The premise of putting seedlings into field cultivation, different seedling hardening methods have a great influence on the survival rate of hardened seedlings and the robustness of seedlings. and the bottleneck of industrialization
During the transplanting and acclimation period of tissue cultured seedlings from sterile heterotrophs to autotrophic bacteria outside the bottle, it is very easy to die a large number of seedlings; or the quality of the seedlings produced is not high, and the effect of field planting is poor, etc.
In conventional seedling hardening, strong light hardening and small arched shed are often used to harden seedlings. The survival rate of transplanted hardened seedlings is about 60%. The slow seedling period is long, and the probability of being infected by root rot and stem rot

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for hardening and transplanting purple-flowered three-fork Dabai and tissue-cultured rooted seedlings, comprising the following steps:

[0037] 1) Seedling hardening in a bottle: Start seedling hardening in September, first disinfect the culture room, and then gradually uncap the culture bottle of the purple flower trident Dabai and tissue culture rooted seedlings in the culture room. The environmental conditions were kept the same as during the tissue culture; then the culture bottle was transferred to the position outside the bottle to harden the seedlings, kept ventilated, controlled the shading over 85%, while keeping the temperature at 20°C and the air humidity above 85%, and transitioned for 5 days;

[0038] 2) Cleaning and soaking: Take out the rooted seedlings of Violet trigeminalis and tissue culture that have been hardened in the bottle from the culture bottle, divide them into individual plants and place them in a culture dish filled with 0.5 cm deep ro...

Embodiment 2

[0045] A method for hardening and transplanting purple-flowered three-fork Dabai and tissue-cultured rooted seedlings, comprising the following steps:

[0046] 1) Seedling hardening in a bottle: Choose to start seedling hardening in October, first disinfect and sterilize the culture room, and then gradually uncover the culture bottles of the purple flower trident and rooted seedlings in the culture room. The environmental conditions were kept the same as during the tissue culture; then the culture bottle was transferred to the position outside the bottle to harden the seedlings, kept ventilated, controlled the shading above 85%, while keeping the temperature at 22°C and the air humidity above 85%, and transitioned for 7 days;

[0047] 2) Cleaning and soaking: take out the rooted seedlings of Violet trident and tissue culture that have been hardened in the bottle from the culture bottle, divide them into individual plants and place them in a culture dish filled with 2cm deep roo...

Embodiment 3

[0054] A method for hardening and transplanting purple-flowered three-fork Dabai and tissue-cultured rooted seedlings, comprising the following steps:

[0055] 1) Seedling hardening in a bottle: Choose to start seedling hardening in February, first disinfect the culture room, and then gradually uncap the culture bottle of the purple flower trident Dabai and tissue culture rooted seedlings in the culture room. The environmental conditions were kept the same as during the tissue culture; then the culture bottle was transferred to the position of seedling hardening outside the bottle, ventilation was maintained, the shading was controlled over 90%, while the temperature was kept at 21°C, the air humidity was over 90%, and the transition culture was carried out for 6 days; The disinfection and sterilization treatment is to use 1100 μW / cm2 ultraviolet light to irradiate and disinfect for 90 minutes in the cultivation room;

[0056] 2) Cleaning and soaking: Take out the rooted seedl...

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PUM

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Abstract

The invention discloses a seedling hardening transplanting method of tissue culture rooting seedlings of Bletilla striata(Thunb.)Reichb.f.. The seedling hardening transplanting method comprises the following steps of 1, conducting seedling hardening in a bottle; 2, conducting cleaning and soaking; 3, conducting seedling hardening outside the bottle, wherein A, seedling hardening at the first proper temperature is conducted, B, seedling hardening at the low temperature is conducted, C, seedling hardening at the second proper temperature is conducted, and D, seedling hardening at the normal temperature is conducted. By means of the seedling hardening transplanting method of the tissue culture rooting seedlings of Bletilla striata(Thunb.)Reichb.f., the infection of tissue culture seedlings byinfectious microbes is greatly lowered, the survival rate is increased, cultivation of healthy seedlings is facilitated, the method is significant in increasing the field planting survival rate and prompting subsequent growth, and thus a basis is laid for improving the product quality and increasing the product yield by a large margin. Grading seedling hardening is conducted, the method has a more scientific adaptation period, the transplanting survival rate is greatly increased, and the seedling recovering time is shortened; the seedling hardening survival rate can reach 99% or above.

Description

technical field [0001] The invention belongs to the technical field of cultivating white plants, and in particular relates to a seedling hardening and transplanting method of purple-flowered three-fork Chinese big white and tissue culture rooted seedlings. Background technique [0002] The purple-flowered three-pointed big white is a species of the orchid plant Bletilla striata (Thunb.) Reichb.f., and there are other species such as yellow flowers, white flowers, and light red flowers, which are also different from the purple-flowered three-pointed small white in Yunnan. There are currently 6 species found in Baiji and 4 species in China, all of which can be used in medicine to varying degrees. However, the best medicinal materials belong to Zihua Sanchabaiji (including Zihua Sanchabaiji and Zihua Sanchaxiaobaiji). The Baiji medicinal materials listed in the 2015 edition of the Chinese Pharmacopoeia describe two conditions in detail, one is purple flowers, and the other is p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/25A01G24/12A01G24/25A01G24/23A01G24/15A01G24/28A01G24/22A01G7/06A01C21/00
CPCA01C21/005A01G7/06
Inventor 王兴瑞李福元王彩丽毕汉专毕汉春徐智生
Owner 云南恩润生物科技发展有限公司
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