A kind of purple flower trigeminal white and tissue culture rooted seedlings for hardening and transplanting method
A purple flower trident and hardening technology, which is applied in gardening methods, fertilization methods, botanical equipment and methods, etc., can solve the problems of high infection rate of stem rot, poor field planting effect, and low transplanting survival rate, etc. Effects of infecting, improving the survival rate of transplanting, and improving the survival rate
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Embodiment 1
[0036] A method for hardening and transplanting purple-flowered three-fork Dabai and tissue-cultured rooted seedlings, comprising the following steps:
[0037] 1) Seedling hardening in a bottle: Start seedling hardening in September, first disinfect the culture room, and then gradually uncap the culture bottle of the purple flower trident Dabai and tissue culture rooted seedlings in the culture room. The environmental conditions were kept the same as during the tissue culture; then the culture bottle was transferred to the position outside the bottle to harden the seedlings, kept ventilated, controlled the shading over 85%, while keeping the temperature at 20°C and the air humidity above 85%, and transitioned for 5 days;
[0038] 2) Cleaning and soaking: Take out the rooted seedlings of Violet trigeminalis and tissue culture that have been hardened in the bottle from the culture bottle, divide them into individual plants and place them in a culture dish filled with 0.5 cm deep ro...
Embodiment 2
[0045] A method for hardening and transplanting purple-flowered three-fork Dabai and tissue-cultured rooted seedlings, comprising the following steps:
[0046] 1) Seedling hardening in a bottle: Choose to start seedling hardening in October, first disinfect and sterilize the culture room, and then gradually uncover the culture bottles of the purple flower trident and rooted seedlings in the culture room. The environmental conditions were kept the same as during the tissue culture; then the culture bottle was transferred to the position outside the bottle to harden the seedlings, kept ventilated, controlled the shading above 85%, while keeping the temperature at 22°C and the air humidity above 85%, and transitioned for 7 days;
[0047] 2) Cleaning and soaking: take out the rooted seedlings of Violet trident and tissue culture that have been hardened in the bottle from the culture bottle, divide them into individual plants and place them in a culture dish filled with 2cm deep roo...
Embodiment 3
[0054] A method for hardening and transplanting purple-flowered three-fork Dabai and tissue-cultured rooted seedlings, comprising the following steps:
[0055] 1) Seedling hardening in a bottle: Choose to start seedling hardening in February, first disinfect the culture room, and then gradually uncap the culture bottle of the purple flower trident Dabai and tissue culture rooted seedlings in the culture room. The environmental conditions were kept the same as during the tissue culture; then the culture bottle was transferred to the position of seedling hardening outside the bottle, ventilation was maintained, the shading was controlled over 90%, while the temperature was kept at 21°C, the air humidity was over 90%, and the transition culture was carried out for 6 days; The disinfection and sterilization treatment is to use 1100 μW / cm2 ultraviolet light to irradiate and disinfect for 90 minutes in the cultivation room;
[0056] 2) Cleaning and soaking: Take out the rooted seedl...
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