Liquid-dropping buffering solution gradient operation system and operation method thereof

Abstract

The invention provides a liquid-dropping buffering solution gradient operation system. The liquid-dropping buffering solution gradient operation system comprises at least four liquid storage bottles for storing a buffering solution, wherein the liquid storage bottles are connected with a proportion control unit, the proportion control unit is connected with a flow control unit, a peristaltic pumpin the flow control unit is connected with a liquid dropping head, and the liquid dropping head is provided with an insertion hole for a Pt electrode and a capillary pipe to insert. An operation method of the liquid-dropping buffering solution gradient operation system comprises the following steps: cleaning and activating a capillary pipe, powering on, preheating, selecting an appropriate buffering solution component according to an experiment target, determining an initial buffering solution formula, preparing, cleaning the Pt electrode, feeding a sample, communicating a capillary electrophoresis apparatus, removing bubbles in the pipeline, setting a flow rate, a gradient parameter and an operation voltage value, starting the high voltage, recording the data, and after the recording, repeating the experiment from the third step; and after the experiment, rinsing the liquid-dropping buffering solution gradient operation system by utilizing pure water, and cleaning the electrode and the capillary tube. The liquid-dropping buffering solution gradient operation system is simple in structure and convenient in operation.

Description

technical field [0001] The patent of the present invention relates to capillary electrophoresis, in particular to a drop-type buffer solution gradient operating system and its operating method. Background technique [0002] The existing capillary electrophoresis system basically adopts a single buffer solution operation mode for sample separation and analysis, which is very unfavorable for the separation and analysis of real complex samples with large differences in properties between components but containing components with similar properties. Such samples have complex components, If a single buffer solution is used, the separation time will be greatly increased, the natural broadening of the chromatographic peak will be intensified, and the separation efficiency will decrease. Even a small number of capillary electrophoresis methods that use gradient buffer solutions also use manual replacement of buffer solutions, which is time-consuming and cumbersome to operate, which ...

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Problems solved by technology

[0003] The invention provides a drop-type buffer solution gradient operation system and its operation method, which solves the problem that the capillary electrophoresis method of the gradient buffer solution adopts the method of manually replacing the buffer solution, which is time-consuming and cumbersome to operate so that the separation stability is greatly reduced. question

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