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A method and application of rapid detection of 6 quality index substances in Ophiopogon japonicus alcohol extract

A technology of quality indicators and material content, applied in the direction of measuring devices, color/spectral characteristic measurement, instruments, etc., can solve the problems of long instrument time, high requirements for instruments and analysts, troublesome pretreatment, etc., to ensure the quality of completion and shorten the time Measuring time and ensuring the effect of product quality

Active Publication Date: 2020-06-30
DALI PHARMA
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above methods have high analysis accuracy, but require analysts to spend a lot of time analyzing them. The method analysis operation is complicated, the instrument takes a long time, the pretreatment is troublesome, and the requirements for instruments and analysts are high, so it is not suitable for real-time analysis and monitoring of the process.

Method used

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  • A method and application of rapid detection of 6 quality index substances in Ophiopogon japonicus alcohol extract
  • A method and application of rapid detection of 6 quality index substances in Ophiopogon japonicus alcohol extract
  • A method and application of rapid detection of 6 quality index substances in Ophiopogon japonicus alcohol extract

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Experimental program
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Effect test

Embodiment 1

[0046] Method flow see figure 1 .

[0047] 1. Collection of calibration set samples:

[0048] Accurately weigh 30g of Ophiopogon japonicus decoction pieces, place them in a glass jacketed extraction tank, add alcohol solution as the extraction solvent, heat and reflux for extraction for a period of time, then collect the filtrate, repeat the above steps, a total of decoction pieces are refluxed and extracted twice, different batches of wheat The amount of ethanol, alcohol concentration and single extraction time in the winter ethanol reflux extraction process were all different. From 10 batches of Ophiopogon japonicus ethanol reflux extraction process, the extraction solution was drawn from time to time, and a total of 14 calibration set samples were obtained;

[0049] 2. Determination of reference values ​​of fructose, glucose and sucrose content in calibration set samples

[0050] High performance liquid chromatography coupled with evaporative light scattering detector (H...

Embodiment 2

[0094] According to the method of Example 1, the difference is that the calculation software Simca-P+12.0 (Umetrics, Sweden) is used instead, and the PLS method is used to establish the quantitative calibration model of 6 quality indicators in the radix radix alcohol extract.

[0095] 1. Use the same calibration set sample as in Example 1, its reference value, and ultraviolet spectrum.

[0096] 2. Establishment of calibration model

[0097] Using Simca to process the raw data, optimize the preprocessing method and band selection of the spectrum, and process the raw data with the optimal band range and sample preprocessing method to obtain the characteristic spectral information of the ethanol extract of Ophiopogon japonicus. The quantitative regression model of six quality indicators was established by PLS method, and the internal validation was carried out by leave-one-out crossover.

[0098] (1) Modeling optimization of different spectral preprocessing methods: SG smoothing...

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Abstract

The invention discloses a method for rapidly detecting contents of six quality index substances in a radix ophiopogonis alcohol extracting solution, belonging to the field of quality monitoring of a traditional Chinese medicine production process. The method comprises the following steps: (1) collecting calibration set samples; (2) determining contents of fructose, glucose, cane sugar, total saponins of radix ophiopogonis, methylophiopogonanone A and methylophiopogonanone B in the calibration set samples by utilizing the traditional method; (3) determining ultraviolet absorption spectrums of the calibration set samples; (4) building a calibration model; (5) verifying the calibration model; and (6) calculating the contents of the six quality index substances in a to-be-detected radix ophiopogonis alcohol extracting solution. According to the method disclosed by the invention, the operation is simple, and the total determination time is shortened by more than 50 times compared with the traditional analytical method; and good correlation exists between ultraviolet characteristic spectrums and the contents of six different quality index substances, the finishing quality of an extraction process is guaranteed by monitoring the quality indexes in real time, and further final product quality is guaranteed.

Description

technical field [0001] The invention relates to the field of quality monitoring in the production process of traditional Chinese medicines, in particular to a method for rapidly detecting the content of six quality index substances in an ethanol extract of Ophiopogon japonicus and its simultaneous determination in the intermediates of an ethanol extraction unit of Ophiopogon japonicus in the production process of Shenmai injection Application of 6 quality indicators. Background technique [0002] Shenmai injection is a pure Chinese medicine quick-acting preparation developed on the basis of the ancient Chinese medicine "Shengmaisan". Clinically, Shenmai Injection is mainly used for the treatment of cardiovascular diseases such as heart failure and arrhythmia, and is also commonly used as an adjuvant drug for antineoplastic drugs. With the wide application of Shenmai injection, people pay more and more attention to the quality control level of its production process. [000...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N21/33
CPCG01N21/33G01N30/88
Inventor 瞿海斌袁玮赵芳张金华李文龙厉明段锦云
Owner DALI PHARMA
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