A group of primers for detecting fⅷ/fⅸ gene mutation in hemophilia patients and its application

Abstract

The invention discloses a set of primers and applications for detecting FVIII / FIX gene mutations in hemophilia patients. The primers are 8 pairs of F9 primers, 39 pairs, 3 22 inversions, and 1 inversion 4 F8 primers , can be used in the etiological classification of hemophilia, according to the etiological classification of hemophilia, non-factor drugs can be used to treat hemophilia, and can make hemophilia patients change from severe to medium, light, acquired FⅧ / FIX : C can return to normal, and further verify that the hemophilia etiology classification method of the present invention is exact and reliable, and for preventing hemophilia hemorrhage and avoiding disability, a new train of thought, new approach have been opened up, and it is helpful for guiding prenatal and postnatal care and blocking inheritance. greater significance.

Description

technical field

[0001] The invention relates to PCR amplification primers in genetic engineering, especially a set of primers for detecting FⅧ / FIX gene mutations in hemophilia patients, and using the primers to classify the etiology of hemophilia, and targeted according to the classification results Treatment for hemophilia. Background technique

[0002] Hemophilia is a group of genetic diseases characterized by three highs (high bleeding rate, high mortality rate, and high disability rate) [1～2] . In the past 20 years, due to the development of molecular biology technology, it has been found that the cause of hemophilia is mainly FⅧ / FⅨ gene mutation [1] , In addition, it was found that a small number of patients had no genetic family history, but the gene mutation of the patient [3] , another 1.5 / 100,000 patients are acquired hemophilia A (Acguired hemophilia A, AHA) [4～5] , but there is still a lack of unified etiological classification criteria for hemophilia, which b...

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