The invention provides a hemostasis composition. The hemostasis composition comprises trypsin and zeolite; pore channels of the zeolite are micropores; the zeolite includes divalent metal positive ions; the mass ratio of the trypsin to the zeolite is 1:200-4:10. According to the hemostasis composition, the trypsin and the zeolite are specifically bound, so that the trypsin keeps a certain conformation on the surface of the zeolite, and higher procoagulant activity is obtained, and thereby, the hemostasis composition with an excellent blood coagulation effect is obtained. The hemostasis composition provided by the invention has a simple preparation method, is low in cost, and convenient to use, can be widely used for traumas and surgical hemostasis, and is especially suitable for emergencyhemostasis of hemophilia patients.