Hemostasis composition and preparation method therefor

A composition and composite material technology, applied in the field of biomedical materials, can solve the problems of poor hemostatic effect, difficult preparation of thrombin fibrin glue, low practical value and commercial value, etc., so as to reduce the risk of continuous bleeding and reduce The cost of hemostatic medication and the effect of shortening the clotting time

A composition and composite material technology, applied in the field of biomedical materials, can solve the problems of poor hemostatic effect, difficult preparation of thrombin fibrin glue, low practical value and commercial value, etc., so as to reduce the risk of continuous bleeding and reduce The cost of hemostatic medication and the effect of shortening the clotting time

CN111249518AActive Publication Date: 2020-06-09ZHEJIANG UNIV
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  • Hemostasis composition and preparation method therefor
  • Hemostasis composition and preparation method therefor
  • Hemostasis composition and preparation method therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Preparation of trypsin A zeolite hemostatic composition:

[0066] ①Impregnate A-type zeolite with a silicon-aluminum ratio of 2 with a 5M magnesium chloride solution at room temperature for 12 hours, and the obtained A-type zeolite contains divalent magnesium ions and accounts for 95% of the metal cation content outside the zeolite framework.

[0067]② Disperse 25 mg of the above-mentioned A-type zeolite in 1 mL of aqueous solution to form a suspension of A-type zeolite; make A-type zeolite fully contact with trypsin (according to the mass ratio of trypsin and A-type zeolite is 1:10) , mixed at 25°C for 30 minutes to adsorb trypsin on the surface of A-type zeolite, and vacuum-dried the above solution at -20°C for 5 hours, and dispersed trypsin on the surface of zeolite to obtain a trypsin-type A zeolite hemostatic composition.

[0068] The normal blood coagulation time of trypsin A zeolite hemostatic composition is 0.4min, and the coagulation time of blood lacking X, VI...

Embodiment 2

[0072] Preparation of trypsin A zeolite hemostatic composition with different divalent cations:

[0073] ① Immerse A-type zeolite with a silicon-aluminum ratio of 2 with one or more solutions of 5M cobalt chloride, nickel chloride, calcium chloride, magnesium chloride or strontium chloride at room temperature for 12 hours to obtain A-type zeolite Each contains different divalent cations.

[0074] 2. Disperse the above-mentioned 25 mg of A-type zeolite containing different divalent cations in 1 mL of aqueous solution to form a suspension of A-type zeolite; make A-type zeolite fully contact with trypsin (the mass ratio of trypsin to A-type zeolite is 1:10), mixed for 30min at 25°C to adsorb trypsin on the surface of zeolite, and dried the above solution in vacuum at -20°C for 5h. Trypsin was dispersed on the surface of zeolite to obtain trypsin A zeolite with different divalent cations Hemostatic composition.

[0075] Different divalent cations (cobalt, nickel, calcium, magnes...

Embodiment 3

[0080] Preparation of trypsin A zeolite hemostatic composition in different proportions:

[0081] ①Impregnate A-type zeolite with a silicon-aluminum ratio of 2 with 5M magnesium chloride solution at room temperature for 12 hours, and the obtained A-type zeolite contains divalent magnesium ions and accounts for 95% of the metal cation content outside the zeolite framework.

[0082] ② Disperse the above-mentioned 25 mg of A-type zeolite in 1 mL aqueous solution evenly to form a suspension of A-type zeolite; make A-type zeolite fully contact with trypsin (the mass ratio of trypsin to A-type zeolite is 1:200, 1 :100, 1:60, 1:50, 1:40, 1:20, 1:10, 1.5:10, 2:10, 2.5:10, 3:10, 4:10), mixed for 30min at 25°C, Trypsin was adsorbed on the surface of zeolite, and the above solution was vacuum-dried at -20° C. for 5 h, and trypsin was dispersed on the surface of zeolite to obtain trypsin A zeolite hemostatic compositions with different ratios (Table 3).

[0083] The coagulation time of t...

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Abstract

The invention provides a hemostasis composition. The hemostasis composition comprises trypsin and zeolite; pore channels of the zeolite are micropores; the zeolite includes divalent metal positive ions; the mass ratio of the trypsin to the zeolite is 1:200-4:10. According to the hemostasis composition, the trypsin and the zeolite are specifically bound, so that the trypsin keeps a certain conformation on the surface of the zeolite, and higher procoagulant activity is obtained, and thereby, the hemostasis composition with an excellent blood coagulation effect is obtained. The hemostasis composition provided by the invention has a simple preparation method, is low in cost, and convenient to use, can be widely used for traumas and surgical hemostasis, and is especially suitable for emergencyhemostasis of hemophilia patients.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a hemostatic composition and a preparation method thereof. Background technique [0002] Humans (including animals) can be injured in a variety of situations. In some cases, the trauma and bleeding are minor and normal hemostasis requires only routine coagulation in addition to the application of simple first aid. Unfortunately, however, profuse bleeding can occur in many accidental situations, for example, from skin cuts or penetrating injuries (caused by knife cuts or bullets) resulting in aortic injury, most of a normal person's blood will be lost in a few minutes Drain and die. Therefore, in the first aid treatment of sudden accidents in daily life, the trauma hemostasis in the operation process of the hospital to the patient, especially in the rescue of the wounded soldiers in the war, it is very important to effectively stop the bleeding of the pa...

Claims

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Application Information

Patent Timeline
09 Jun 2020
Publication
CN111249518A
IPC
A61L24/02; A61L24/10; A61L24/00
CPC
A61L24/108; A61L24/02; A61L24/001; C08L89/00; A61L24/08; A61L24/0068; A61L24/0015; A61L15/18
Inventors
范杰; 余丽莎