Hemostasis composition and preparation method therefor

Abstract

The invention provides a hemostasis composition. The hemostasis composition comprises trypsin and zeolite; pore channels of the zeolite are micropores; the zeolite includes divalent metal positive ions; the mass ratio of the trypsin to the zeolite is 1:200-4:10. According to the hemostasis composition, the trypsin and the zeolite are specifically bound, so that the trypsin keeps a certain conformation on the surface of the zeolite, and higher procoagulant activity is obtained, and thereby, the hemostasis composition with an excellent blood coagulation effect is obtained. The hemostasis composition provided by the invention has a simple preparation method, is low in cost, and convenient to use, can be widely used for traumas and surgical hemostasis, and is especially suitable for emergencyhemostasis of hemophilia patients.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a hemostatic composition and a preparation method thereof. Background technique [0002] Humans (including animals) can be injured in a variety of situations. In some cases, the trauma and bleeding are minor and normal hemostasis requires only routine coagulation in addition to the application of simple first aid. Unfortunately, however, profuse bleeding can occur in many accidental situations, for example, from skin cuts or penetrating injuries (caused by knife cuts or bullets) resulting in aortic injury, most of a normal person's blood will be lost in a few minutes Drain and die. Therefore, in the first aid treatment of sudden accidents in daily life, the trauma hemostasis in the operation process of the hospital to the patient, especially in the rescue of the wounded soldiers in the war, it is very important to effectively stop the bleeding of the pa...

AI Technical Summary

Problems solved by technology

However, thrombin or fibrin glue is difficult to prepare, requires high purity, and storage conditions (-20°C) are harsh, and the cost is very high (the market price of thrombin is generally 2000 yuan / mg; the market price of fibrin glue is generally 8000 yuan / small package, one-time use), thus resulting in low practical value and commercial value

[0005] Zeolite is a commonly used inorganic material, and enzyme is a biological material that promotes biological reactions. There are great challenges in combining inorganic and biological materials. The main reason is that zeolite is a "rigid" inorganic material, and enzymes are "Flexible" biological macromolecules, when the enzyme is in contact with the surface of the zeolite, the rigid zeolite surface is easy to change the "flexible" characteristics of the enzyme, that is, the conformation changes; and the enzyme combined with the zeolite, a part of the enzyme and Zeolite surface contact, resulting in a reduced possibility of contact with reactants, that is, the reaction rate is slowed down, and the combination of enzymes and zeolites reduces the activity of enzymes

Therefore, the introduction of enzymes on the surface of zeolite will lead to different degrees of inactivation of enzymes on the surface of zeolite

For example: zeolite is an inorganic hemostatic material, and thrombin is a highly efficient biological hemostatic agent; but when the two are combined, most of the thrombin (94%) is inactivated on the surface of the zeolite, and the coagulation effect of thrombin is extended from 0.5min to 2.2min( Figure 4 ), the hemostatic effect is not good, and it is far from meeting the requirements of emergency hemostasis

At present, the existing technology has not yet found an inorganic bio-enzyme-bound hemostatic material that can stop bleeding quickly, with low cost, and is effective for hemophilia

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