18 results about "Estrogens catechol" patented technology

The invention belongs to the field of pharmaceutical preparations, and provides a medical composition for treating premature delivery and a preparation method thereof. The progesterone nanocrystal (D50 is 250-350 nm) and poloxamer 407 / 188 thermosensitive gel-forming system composite design is adopted, hydroxypropyl methylcellulose or carbomer 974P and a functional adhesion polymer (carbomer-cysteine or carbomer-catechol) are matched, the pH is adjusted to 4.0-4.5 through a lactic acid / sodium lactate buffer system, and the progesterone nanocrystal / poloxamer composite gel-forming agent is prepared. The excellent performance that the sol-gel transition temperature is 30-34 DEG C, the elastic modulus is not smaller than 150 Pa at 37 DEG C, the modulus retention rate is not smaller than 60% after the emulsion is diluted by 5-10 times, the adhesion work is not smaller than 0.60 N.s, and the anti-flushing retention rate is not smaller than 70% after the emulsion is diluted by 5-10 times is achieved. The invention solves the problem of synergy of high-modulus gel strength, rapid self-repairing ability and anti-dilution and anti-flushing stability, realizes unification of long-acting drug release and excellent biological adhesion performance in vagina physiological fluid dilution and mechanical flushing environments, and has wide clinical application value.

The application discloses a method for constructing a social novelty defect model through catechol exposure of pregnant mice, and belongs to the technical field of disease animal models. The method comprises the following steps: performing catechol exposure treatment on the pregnant mice, feeding the pregnant mice until childbirth, and waiting for the obtained offspring mice to grow to a required age, so as to obtain the social novelty defect model. The method is capable of precisely matching a key time window of embryonic neural development, and adopts a single catechol exposure mode, so that the method is simple to operate, has a high model formation rate, and has a high survival rate of the mother mice and the offspring. In addition, the induced social novelty defect model of the offspring mice is stable in phenotype and strong in specificity, can simulate the cross-generation influence of maternal pregnancy environment exposure on abnormal social functions of offspring young, and is suitable for mechanism research, drug screening and diagnosis marker development of social impairment diseases such as autism.

Described are conjugates comprising a water-soluble polymer linked to a compound comprising a catechol moiety via a cleavable linkage, wherein the cleavable linkage is formed between the water-soluble polymer and a first phenolic hydroxyl group of the catechol moiety and a second phenolic hydroxyl group of the catechol moiety is linked to a blocking group wherein the rate of hydrolytic release of the compound comprising the catechol moiety is controlled, at least in part, through structure or design of the blocking group on the second phenolic hydroxyl group of the catechol moiety. Therefore, the rate of hydrolytic release of the compound comprising the catechol moiety can be tuned through structural design of the group on the second phenolic hydroxyl group of the catechol moiety. Compounds used in the synthesis of the described conjugates and methods of using the described conjugate and other compounds in the treatment of dopamine-responsive disorders are also described.

The application relates to a composite nanogel and a preparation method and application thereof, in particular to the technical field of biomedical materials. The composite nanogel comprises a nanogel framework and tirapazamine and folic acid loaded on the nanogel framework, wherein raw material components of the nanogel framework comprise a temperature-sensitive monomer and a monomer containing a catechol group. The composite nanogel provided by the application can solve the problems that poor blood vessel adhesion of traditional embolic agents easily leads to embolism recanalization, simple physical embolism is difficult to completely remove hypoxic tumor cells, and existing treatment strategies cannot effectively reverse the immune suppression microenvironment after embolism and activate the systemic anti-tumor immune response.

The invention provides a catechol-O-methyltransferase mutant as well as a preparation method and application thereof, and relates to the technical field of biology. The catechol-O-methyltransferase mutant contains 79P mutation relative to wild type catechol-O-methyltransferase, the catechol-O-methyltransferase mutant can be expressed in vitro through recombinant cells, and the problems that in the prior art, a catechol-O-methyltransferase preparation process is poor in stability and high in cost are solved.

The invention relates to a preparation method of microenvironment response type allosteric cross-linked oral nanogel. The preparation method comprises the following steps: S1, synthesizing a catechol modified HA molecular precursor HA-DA; s2, synthesizing a Se-HA-DA conjugate cross-linked with a diselenide bond and a C-HA-DA conjugate cross-linked with a carbon bond by taking the HA-DA dialyzed and acidified in 0.01 M HCl as a substrate; and S3, preparing the endogenous response'allosteric-crosslinking 'nanogel carrying the DNase I by taking the Se-HA-DA conjugate cross-linked with the selenium bond or the C-HA-DA conjugate cross-linked with the carbon bond as a precursor substance of the nanogel. Due to the adoption of the technical scheme, too high barrier rigidity caused by an irreversible cross-linking structure can be reduced, and the mechanical requirement of tissue repair is adapted through a dynamic cross-linking network in the process of allosteric and cross-linking. The invention relates to an application of a microenvironment response type allosteric cross-linked oral nanogel.

The invention relates to composite nanogel as well as a preparation method and application thereof, in particular to the technical field of biomedical materials. The composite nanogel comprises a nanogel skeleton, tirapazamine and folic acid, the tirapazamine and the folic acid are loaded on the nanogel skeleton, and raw material components of the nanogel skeleton comprise a temperature-sensitive monomer and a monomer containing a catechol group. The composite nanogel provided by the invention can solve the problems that a traditional embolism agent is poor in blood vessel adhesion and easy to cause embolism recovery, hypoxia tumor cells are difficult to thoroughly remove by pure physical embolism, and an immunosuppressive microenvironment after embolism cannot be effectively reversed and systemic anti-tumor immune response cannot be activated by an existing treatment strategy.

Bioinspired adhesives comprising a macromolecule such as zein and one or more catechol- or gallol-containing compounds are provided. The adhesive can also comprise an iron crosslinker, an interfacial crosslinker, or both an iron crosslinker and an interfacial crosslinker. Methods of manufacturing and using the adhesive are also provided.

There are described compositions comprising an effective amount of a combination of two or more components, said components selected from acacetin, ACTI peptide, alpha-lipolic acid, alprostadil, anisomycin, apigenin, ascorbic acid, astragalus, berberine, β-lapachone, β-hydroxy-beta-methyl-butyrate, Bacopa monnieri, catechin, catechol, chamomile, chrysin, coumestrol, curcumin, dinitrophenol, dinoprost, ellagic acid, (−)-epigallocatechin gallate, green tea extract, fisetin, genistein, ginsenoside RE, glabridin, 18-α-glycyrrhetinic acid, 18-β-glycyrrhetinic acid, glycyrrhizin, hydroquinone, isoquercitrin (EMIQ), kaempferol, kuromanin, leucine, lithium, luteolin, luteolin, luteolinidin, melatonin, menadione, 1-methylnicotinamide (MNA), methyl salicylate, myricetin, nadide, niacin (vitamin B3), nicotinamide (NAM), nicotinamide mononucleotide (NMN), nicotinamide riboside (NR), nicotinic acid adenine dinucleotide (NaAD), nicotinic acid mononucleotide (NaMN), parsley (Petroselinium crispum), phenylephrine, pokeweed mitogen, 15-Δ prostaglandin J2, puromycin, quercetin, quinolinic acid, retinoic acid, trichostatin A, troxrutin, rutin, tryptophan, vitamin D3, withaferin A, wortmannin and zinc (including salts thereof).

The present application relates to catechol O The present application relates to a vanillin production method, and belongs to the technical field of metabolic engineering. The present application uses catechol methyltransferase mutants to produce vanillin. Leptospira interrogans ​ The present application relates to a vanillin production method, and belongs to the technical field of metabolic engineering. The present application uses catechol methyltransferase mutants to produce vanillin. O The present application relates to a vanillin production method, and belongs to the technical field of metabolic engineering. The present application uses catechol methyltransferase mutants to produce vanillin. The present application relates to a vanillin production method, and belongs to the technical field of metabolic engineering. The present application uses catechol methyltransferase mutants to produce vanillin.

The invention discloses an in-situ adhesion gel filler with biofilm attacking and dual osteogenesis promoting functions as well as a preparation method and application of the in-situ adhesion gel filler. Comprising the following steps: 1) preparing a pre-solution by using methacrylic acylated hyaluronic acid, catechol group and nitrobenzene group modified hyaluronic acid and a photoinitiator, and adding a traditional Chinese medicine antibacterial agent; (2) separately loading the antibacterial peptide and the osteogenesis promoting polypeptide in the mesoporous zinc oxide Meso-ZnO by adopting an ultrasonic impregnation method; (3) preparing a composite methacrylated silk fibroin microsphere containing Meso-ZnO loaded with the osteogenesis promoting polypeptide by using a micro-fluidic technology; 4, EPS hydrolase, Meso-ZnO loaded with antibacterial peptide and composite microspheres are added into the cooled pre-solution, an in-situ adhesion gel filler is obtained, and the in-situ adhesion gel filler is cured through a dual UV in-situ curing technology.The in-situ adhesion gel filler has the advantages that biological membrane extracellular matrixes are destroyed, free bacteria are killed, and bone repair is promoted.

The application provides a hydrogel stone-dissolving stent and a preparation method and application thereof. The stent is a gamma-polyglutamic acid / polyethyleneimine hydrogel coated with a stone-dissolving drug, citric acid, on the outer surface of a bare stent in a pancreatic duct. The hydrogel stone-dissolving stent prepared by the application has good surface viscoelasticity, soft texture, strong water absorption and good biocompatibility, can gradually swell in the pancreatic duct and slowly release citric acid, and finally achieve the purpose of stone dissolution. In order to prevent the hydrogel from falling off, the dopamine is first oxidized and self-polymerized in a Tris-HCl solution to oxidize catechol to catecholquinone, so as to perform surface modification on the pancreatic duct stent, covalently bond the benzenequinone group with amino groups in the gamma-polyglutamic acid / polyethyleneimine hydrogel, and then the hydrogel and the stent can be more firmly attached. Meanwhile, the stent has good pressure resistance and considerable in-vitro stone-dissolving effect.

Disclosed are method for predicting naltrexone response in subjects with AUD. In some embodiments, the methods include performing or having performed one or more methylation assays on a genomic DNA sample isolated from the subject to determine the methylation status of one or more regions of the isolated genomic DNA, wherein the one or more regions are subsequences of a gene selected from a mu opioid receptor (OPRM1) gene, a catechol-O-methyltransferase (COMT) gene, and a dopamine transporter (SLC6A3) gene, wherein the methylation status of the one or more regions of the isolated genomic DNA determined is predictive of naltrexone response in the subject. Also provided are method for treating patients diagnosed with AUD with the medication naltrexone based on a methylation status of a combination of specific methylation sites located associated with an OPRM1 gene, a COMT gene, and / or an SLC6A3 gene in obtained from each AUD patient.

The invention relates to a phenolic acid-strontium nano-particle and a preparation method and application thereof, the phenolic acid-strontium nano-particle is a nano-structure obtained by self-assembly by using a method of modifying phenolic acid by metal strontium coordination, according to the prepared caffeic acid-strontium nanoparticles, coordination modification of phenolic acid molecules is achieved through coordination of strontium, carboxyl of phenolic acid and catechol groups, and the caffeic acid-strontium nanoparticles are of a spherical structure, small and uniform in particle size, good in dispersity, simple in preparation process, low in cost and easy to produce in batches; after the caffeic acid is subjected to coordination modification by strontium, the capability of targeted inhibition of the caffeic acid on the TRPV1 is remarkably enhanced, so that the problem of calcium overload of a focus is solved, and the problems of inflammatory polarization of macrophages and sensitization of damaged sensory neurons, which are caused by abnormal calcium influx due to high expression of the TRPV1 at the arthritis focus part, are effectively and synchronously solved; and the compound has excellent bone repair performance and extremely high biological safety, and has wide application prospects in preparation of drugs for preventing and / or treating inflammatory bone related diseases and preparation of bone repair drugs.

The invention discloses a preparation method of a multifunctional hemostatic dressing suitable for multiple external chest injuries. The preparation method comprises the following six steps: preparation of amino modified chitosan, preparation of nanoparticles loaded with blood coagulation factors, preparation of a catechol-sodium alginate composite adhesive, improvement of the performance of a base material through electrode treatment, preparation of a porous hydrophilic layer and composite molding. The method integrates an electrode treatment technology, effectively eliminates the internal stress of the material, and improves the adhesiveness and stability; in combination with core components such as modified chitosan and blood coagulation factor nanoparticles, the dressing is endowed with the functions of quickly stopping bleeding, strongly adhering in a wet state, inhibiting bacteria, resisting infection, promoting healing and the like. The finished dressing is good in biocompatibility, free of sensitization and irritation and excellent in mechanical property, the complex hemostasis requirement for multiple external chest injuries can be met in a targeted mode, the preparation technology is controllable, and the dressing is suitable for large-scale production.

The invention discloses a nursing dressing for promoting scar healing in nail and breast surgery and a preparation method thereof, and belongs to the technical field of medical dressings. According to the dressing, oxidized sodium hyaluronate, quaternized chitosan, oxidized catechin and zinc sulfate serve as main raw materials, oxidized catechin serves as a multifunctional cross-linking node and is subjected to a Michael addition reaction with a macromolecular chain, stable metal-catechol coordination bonds are formed with zinc ions, Schiff base reaction and ionic cross-linking are cooperated, and the dressing is prepared. And a quadruple dynamic stable network is constructed. The preparation method comprises the steps of raw material solution preparation, step-by-step chemical crosslinking and gel curing forming. The dressing has efficient antibacterial, anti-inflammatory and antioxidant functions, can promote ordered arrangement of collagen and migration of fibroblasts, realizes controlled-release delivery of active ingredients, remarkably inhibits postoperative scar hyperplasia, and is suitable for comprehensive nursing of postoperative wounds of thyroid and breast surgery such as thyroid resection and breast tumor resection.

Methods and consumer products for detecting a metabolite, the method comprising: testing a urine sample from a subject for a metabolite selected from the group consisting of ascorbate, dehydroascorbate, CEHC-sulfate, CEHC-taurine, CEHC-glucronide, pantoate, 5-amino valerate, galactonate, phenylacetylalanine, methylcatecholsulfate, phenylacetylglutamine, carboxysuccinate, carboxyethylvaline, arabinose, threonate, methylcrotonylglycine, glucuronate, carboxyethylisoleucine, glycoursodeoxycholate, leucylalanine, lithocholatesulfate, alio-threonine, cholic, glucuronide and combinations thereof; and producing a metabolic profile for the metabolite.

The invention provides a preparation method of (+ / -)-noradrenaline. Comprising the following steps: (a) adding 1-(3, 4-dihydroxyphenyl)-2-aminoethanone and Bronsted acid into a polar protic solvent, and heating to dissolve the 1-(3, 4-dihydroxyphenyl)-2-aminoethanone and the Bronsted acid; (b) transferring the solution obtained in the step (a) into a hydrogenation bottle, firstly adding palladium / carbon, then replacing gas in the bottle, hydrogenating to 3-5 atmospheric pressures, controlling the internal temperature to be 35-50 DEG C, and keeping for 2-5 hours; and (c) after the reaction is finished, filtering, cooling the filtrate, dropwise adding ammonia water until the pH value of the reaction liquid is 9-10, filtering the separated solid, and drying to obtain the (+ / -)-noradrenaline. According to the invention, an intermediate 1-(3, 4-dihydroxyphenyl)-2-aminoethanone obtained by substitution and hydrolysis of chloroacetyl catechol is used as a raw material, and (+ / -)-noradrenaline is prepared by hydrogenation in a specific polar protic solvent through Bronsted acid activation and palladium-carbon catalysis. The technological process is simple and convenient, the reaction condition is mild, the reaction is rapid, the requirement for equipment is low, and the obtained product is high in yield, high in purity, few in by-product and especially suitable for large-scale industrial production.