A kind of rapid propagation method of Taiwan banyan seed tissue culture

A technology of seed group and Taiwan ficus, applied in the field of agricultural biology, can solve the problems that the number of seedlings cannot meet the requirements of large-scale planting, the reproduction coefficient is low, and the number of picking ears is limited.

Active Publication Date: 2021-02-05
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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Problems solved by technology

Taiwan banyan seeds have deep dormancy and low reproduction coefficient. At present, Taiwan banyan is mostly artificially propagated by cuttings. Because the number of ear picking is limited and cuttings are affected by seasons, the number of seedlings can not meet the needs of large-scale planting.

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  • A kind of rapid propagation method of Taiwan banyan seed tissue culture
  • A kind of rapid propagation method of Taiwan banyan seed tissue culture
  • A kind of rapid propagation method of Taiwan banyan seed tissue culture

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Embodiment Construction

[0024] Preferred embodiments of the present invention are described in further detail below:

[0025] A method for rapid propagation of Ficus taiwanensis tissue culture, comprising the following steps:

[0026] (1) Environmental disinfection: according to 5-8ml / m 3 , 5ml / m 3 Weigh 40% formaldehyde and potassium permanganate and pour them into cans in turn, use the generated smoke to seal the fumigation inoculation room for 3-4 days, then open the door and let the formaldehyde gas go. In order to inoculate as soon as possible, you can Use ammonia water to neutralize formaldehyde; turn on the ultraviolet lamp before inoculation, irradiate for 20-30 minutes, then spray the ultra-clean work surface evenly with 75% alcohol, and wipe it clean with sterilized gauze.

[0027] (2) Selection of explants: select the mature seeds that are strong and plump in the wild Ficus taiwanensis

[0028] (3) Disinfection of explants: use mature wild Taiwanese seeds as explants, add appropriate am...

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Abstract

The invention provides a method for tissue culture and rapid propagation of Ficus taiwanensis seeds, comprising the following steps: step (1) environmental disinfection; step (2) selection of explants; step (3) disinfection of explants; step (4) cultivation Base configuration; step (5) callus induction; step (6) callus differentiation; step (7) adventitious bud proliferation; step (8) rooting culture; step (9) culture conditions; step (10) hardening and transplanting plant. The present invention studies the effects of different disinfection methods on the sterilizing effect of Ficus taiwanensis seeds and the ratio of different growth regulators on the callus induction, differentiation of adventitious buds and rooting of Ficus taiwanensis seeds, and provides the optimal scheme with simple formula, The effect is obvious, it overcomes the deficiency of Taiwan Ficus cutting propagation, and accelerates the breeding and popularization speed of this variety.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of Ficus taiwanensis seeds. Background technique [0002] Taiwan banyan, also known as long-leaf milk tree, star wood, and water betel nut, is a shrub of the genus Moraceae. It is distributed in Taiwan, Vietnam, Guizhou, Guangxi, Guangdong and other places in China. It often grows wild on hillsides at an altitude of 200 meters to 1,000 meters. Or in a damp place next to a stream, the mature plant height can reach 2-3 meters. The roots, bark, leaves and fruits of the Ficus taiwanensis are the raw materials for pharmaceuticals, which have the functions of promoting blood circulation, relaxing tendons and collaterals, dispelling wind and dampness, and clearing away heat and detoxification. In addition, the root system of Taiwan banyan is well developed, and it does not have high requirements on the soil. It can also be deve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 杨鑫谭冠宁李丽淑何虎翼唐洲萍樊吴静何新民
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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