Tissue-culture and fast-propagation method of semen fici formosanae

A seed group, the technology of Taiwan banyan, applied in the field of agricultural biology, can solve the problems of limited number of ear picking, low reproduction coefficient, and the number of seedlings cannot meet large-scale planting

Active Publication Date: 2018-07-31
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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Problems solved by technology

Taiwan banyan seeds have deep dormancy and low reproduction coefficient. At present, Taiwan banyan is mostly artificially propagated by cuttings. Because the number of ear picking is limited and cuttings are affected by seasons, the number of seedlings can not meet the needs of large-scale planting.

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  • Tissue-culture and fast-propagation method of semen fici formosanae
  • Tissue-culture and fast-propagation method of semen fici formosanae
  • Tissue-culture and fast-propagation method of semen fici formosanae

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Embodiment Construction

[0024] Preferred embodiments of the present invention are described in further detail below:

[0025] A method for rapid propagation of Ficus taiwanensis tissue culture, comprising the following steps:

[0026] (1) Environmental disinfection: according to 5-8ml / m 3 , 5ml / m 3 Weigh 40% formaldehyde and potassium permanganate and pour them into cans in turn, use the generated smoke to seal the fumigation inoculation room for 3-4 days, then open the door and let the formaldehyde gas go. In order to inoculate as soon as possible, you can Use ammonia water to neutralize formaldehyde; turn on the ultraviolet lamp before inoculation, irradiate for 20-30 minutes, then spray the ultra-clean work surface evenly with 75% alcohol, and wipe it clean with sterilized gauze.

[0027] (2) Selection of explants: select the mature seeds that are strong and plump in the wild Ficus taiwanensis

[0028] (3) Disinfection of explants: use mature wild Taiwanese seeds as explants, add appropriate am...

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Abstract

The invention provides a tissue-culture and fast-propagation method of semen fici formosanae. The tissue-culture and fast-propagation method comprises the following steps of: (1) environment disinfection; (2) choose of explants; (3) disinfection of disinfection; (4) preparation of culture medium; (5) induction of callus; (6) differentiation of callus; (7) proliferation of adventitious buds; (8) rooting culture; (9) culture conditions; (10) hardening and transplantation. The tissue-culture and fast-propagation method researches the sterilization effect of different disinfection modes for the semen fici formosanae and the influence of the matching ratio of different growth regulators on induction of the callus and differentiation and rooting of the adventitious buds of the semen fici formosanae and gives out an optimum scheme, and has the beneficial effects that the formula is simple, the effect is obvious, the defects of cutting propagation of ficus formosana are overcome and the speedof breeding and promotion of the varieties is accelerated.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of Ficus taiwanensis seeds. Background technique [0002] Taiwan banyan, also known as long-leaf milk tree, star wood, and water betel nut, is a shrub of the genus Moraceae. It is distributed in Taiwan, Vietnam, Guizhou, Guangxi, Guangdong and other places in China. It often grows wild on hillsides at an altitude of 200 meters to 1,000 meters. Or in a damp place next to a stream, the mature plant height can reach 2-3 meters. The roots, bark, leaves and fruits of the Ficus taiwanensis are the raw materials for pharmaceuticals, which have the functions of promoting blood circulation, relaxing tendons and collaterals, dispelling wind and dampness, and clearing away heat and detoxification. In addition, the root system of Taiwan banyan is well developed, and it does not have high requirements on the soil. It can also be deve...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 杨鑫谭冠宁李丽淑何虎翼唐洲萍樊吴静何新民
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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