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Detection kit for oral squamous cell carcinoma lymph node metastasis prediction

A technology of lymph node metastasis and detection kit, which is applied in the direction of microbial measurement/testing, biochemical equipment and methods, etc. It can solve the problems of affecting the results and reducing the sensitivity of the experiment, and achieve the effect of improving the survival rate

Active Publication Date: 2018-09-18
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Problems solved by technology

[0005] At present, the mainstream method for screening candidate marker gene molecules is generally a high-throughput method for initial screening, and then real-time polymerase chain reaction for verification. The method is stable and technically mature, but it is also prone to some false positive results; using probes The detection of candidate gene molecules by this method will not only improve the detection specificity, but also reduce the experimental sensitivity. To a certain extent, there will be a missed diagnosis rate, and there will be some differences between different batches of samples and experimental reagents, which will affect the results.

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  • Detection kit for oral squamous cell carcinoma lymph node metastasis prediction
  • Detection kit for oral squamous cell carcinoma lymph node metastasis prediction
  • Detection kit for oral squamous cell carcinoma lymph node metastasis prediction

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[0025] The following describes the preferred embodiments of the present invention with reference to the accompanying drawings to make the technical content clearer and easier to understand. The present invention can be embodied in many different forms of embodiments, and the protection scope of the present invention is not limited to the embodiments mentioned herein.

[0026] The kit in this embodiment includes total RNA (ribonucleic acid) extraction reagent (manufacturer: Thermo Fisher Scientific (China) Co., Ltd., article number: 15596018), PCR reverse transcription reagent (manufacturer: Thermo Fisher Technology (China) Co., Ltd., product number: 4374967), PCR quantitative reagent (manufacturer: Thermo Fisher Scientific (China) Co., Ltd., product number: 11736059). The kit also includes the upstream and downstream primer sequences of the following 78 oral squamous cell carcinoma-related differentially expressed genes designed by Primer 5.0 software, and the β-actin housekee...

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Abstract

The invention relates to the technical field of gene detection, and discloses a detection kit for oral squamous cell carcinoma lymph node metastasis prediction. The kit comprises forward primer and reverse primer sequences of 7 kinds of genes including PDGFRA, PLAU, SOX9, SPP1, CDKN2A, CASP1 and MAL. The invention also discloses a method for screening the gene or the gene combination for the oralsquamous cell carcinoma lymph node metastasis prediction. The detection kit can be used for objectively, accurately and sensitively predicting the oral squamous cell carcinoma lymph node metastasis condition, so that the survival rate of the oral squamous cell carcinoma patients can be favorably improved.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to a detection kit for the prediction of lymph node metastasis of oral squamous cell carcinoma. Background technique [0002] Oral squamous cell carcinoma is one of the most common malignant tumors of the oral and maxillofacial region. The 5-year survival rate of advanced oral squamous cell carcinoma is low, and lymph node metastasis is an independent predictor for survival prediction of patients with oral squamous cell carcinoma. Improving the prediction level of lymph node metastasis in patients with oral squamous cell carcinoma is one of the main ways to improve the survival rate of patients with oral squamous cell carcinoma clinically. [0003] At present, the prediction of lymph node metastasis of oral squamous cell carcinoma still relies on imaging and the detection of clinical specialists. Due to differences in the professional level of physicians in regions and train...

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/158
Inventor 曹巍陈万涛邹欣冯芝恩吴坤
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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