Whole Genome Sequence, Primers and Application of Artificially Attenuated Strain b of Foot-and-Mouth Disease Virus
A foot-and-mouth disease virus, artificial technology, applied in the field of molecular biology, can solve problems such as inability to rescue the virus, difficulty in studying molecular pathogenic mechanisms, inability to understand the biological characteristics of the virus growth curve, virus titer, pathogenicity, etc., and achieve broad Effects of Research and Industrialization Prospects
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Embodiment 1
[0026] Example 1: The whole genome sequence of artificially attenuated foot-and-mouth disease virus strain O / Akesu / 58CE39B, as shown in SEQ ID No.1.
Embodiment 2
[0027] Embodiment 2: the assay method of foot-and-mouth disease virus artificially attenuated strain O / Akesu / 58CE39B complete genome sequence, carry out as follows: the first step, with foot-and-mouth disease virus artificially attenuated strain O / Akesu / 58CE39B cDNA as template, use SEQ The primers shown in ID No.2 to SEQ ID No.15 amplify 7 fragments of the whole genome of the artificially attenuated strain O / Akesu / 58CE39B of foot-and-mouth disease virus; the second step, after the amplification, the target fragments are recovered and identified by TA cloning, Connected into the sequencing vector pMD19-T for sequencing; the third step, after sequencing, sequentially spliced, edited and corrected the overlapping parts of the DNA sequences of the 7 fragments to obtain the whole genome sequence of the artificially attenuated strain of foot-and-mouth disease virus O / Akesu / 58CE39B .
Embodiment 3
[0028] Example 3: Primers for amplifying the whole genome sequence of artificially attenuated foot-and-mouth disease virus strain O / Akesu / 58CE39B, the sequences of which are shown in SEQ ID No.2 to SEQ ID No.15.
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