Whole Genome Sequence, Primers and Application of Artificially Attenuated Strain b of Foot-and-Mouth Disease Virus

A foot-and-mouth disease virus, artificial technology, applied in the field of molecular biology, can solve problems such as inability to rescue the virus, difficulty in studying molecular pathogenic mechanisms, inability to understand the biological characteristics of the virus growth curve, virus titer, pathogenicity, etc., and achieve broad Effects of Research and Industrialization Prospects

Active Publication Date: 2022-02-22
VETERINARY INST XINJINAG ACADEMY OF ANIMAL SCI CLINIC MEDICAL SCI RES CENT XINJIANG ACADEMY OF ANIMAL HUSBANDRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The different topological sequences of each serotype of FMD virus are incomplete, making it difficult to study the molecular pathogenic mechanism of different topological FMD viruses
Because there is no report on the full genome sequence of the artificially attenuated strain O / Akesu / 58CE39B of FMD virus, it is impossible to understand the biological characteristics of the virus such as growth curve, virus titer, and pathogenicity, and it is also impossible to perform reverse genetic manipulation. system get rescue virus

Method used

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  • Whole Genome Sequence, Primers and Application of Artificially Attenuated Strain b of Foot-and-Mouth Disease Virus
  • Whole Genome Sequence, Primers and Application of Artificially Attenuated Strain b of Foot-and-Mouth Disease Virus
  • Whole Genome Sequence, Primers and Application of Artificially Attenuated Strain b of Foot-and-Mouth Disease Virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: The whole genome sequence of artificially attenuated foot-and-mouth disease virus strain O / Akesu / 58CE39B, as shown in SEQ ID No.1.

Embodiment 2

[0027] Embodiment 2: the assay method of foot-and-mouth disease virus artificially attenuated strain O / Akesu / 58CE39B complete genome sequence, carry out as follows: the first step, with foot-and-mouth disease virus artificially attenuated strain O / Akesu / 58CE39B cDNA as template, use SEQ The primers shown in ID No.2 to SEQ ID No.15 amplify 7 fragments of the whole genome of the artificially attenuated strain O / Akesu / 58CE39B of foot-and-mouth disease virus; the second step, after the amplification, the target fragments are recovered and identified by TA cloning, Connected into the sequencing vector pMD19-T for sequencing; the third step, after sequencing, sequentially spliced, edited and corrected the overlapping parts of the DNA sequences of the 7 fragments to obtain the whole genome sequence of the artificially attenuated strain of foot-and-mouth disease virus O / Akesu / 58CE39B .

Embodiment 3

[0028] Example 3: Primers for amplifying the whole genome sequence of artificially attenuated foot-and-mouth disease virus strain O / Akesu / 58CE39B, the sequences of which are shown in SEQ ID No.2 to SEQ ID No.15.

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Abstract

The present invention relates to the technical field of molecular biology, and relates to an artificial attenuated strain O / Akesu / 58 CE39B genome sequence, primers and applications thereof. The artificially attenuated strain O / Akesu / 58 CE39B genome sequence is as follows: Shown in SEQ ID No.1. The present invention discloses the full genome sequence of the artificially attenuated strain O / Akesu / 58 CE39B of foot-and-mouth disease virus for the first time, which is helpful for the reverse genetics research of the virus, and is for further research on the proliferation mechanism, virus titer and pathogenic mechanism of FMDV in cells It lays the foundation for FMDV-marked vaccines, which is conducive to the study of virus evolution, pathogenicity, and molecular epidemiology; and the artificially attenuated strain of foot-and-mouth disease virus O / Akesu / 58 CE39B rescued virus has good safety and effectiveness, and is expected to be used as a vaccine. The next-generation genetically engineered vaccine is used for the prevention and control of foot-and-mouth disease, and has broad prospects for research and industrialization.

Description

technical field [0001] The invention relates to the technical field of molecular biology, and relates to a whole genome sequence of the artificially attenuated strain O / Akesu / 58CE39B of foot-and-mouth disease virus, and the application of the whole genome sequence of the artificially attenuated strain O / Akesu / 58CE39B of foot-and-mouth disease virus in rescuing foot-and-mouth disease virus It also includes a method for determining the whole genome sequence of the artificially attenuated strain O / Akesu / 58CE39B of foot-and-mouth disease virus; it also includes a primer for amplifying the whole genome sequence of the artificially attenuated strain O / Akesu / 58CE39B of the foot-and-mouth disease virus, The primers are used as preparations or kits for preparing and amplifying the whole genome sequence of the artificial attenuated strain O / Akesu / 58CE39B of foot-and-mouth disease virus; Application of artificially attenuated strain O / Akesu / 58CE39B in rescuing O-type foot-and-mouth disea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/42C12Q1/6869C12N15/11C12N7/00C12N7/04A61K39/135A61P31/14
CPCA61K39/12A61P31/14C12N7/00C12Q1/6869C07K14/005C12N2770/32121C12N2770/32122C12N2770/32134C12N2770/32162
Inventor 苗书魁马文戈汪萍魏玉荣米晓云魏婕夏俊陆桂丽沙依兰·卡依扎王延薛英黄炯
Owner VETERINARY INST XINJINAG ACADEMY OF ANIMAL SCI CLINIC MEDICAL SCI RES CENT XINJIANG ACADEMY OF ANIMAL HUSBANDRY SCI
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