Device for ultrasound microbubble-mediated transfection

Abstract

The invention discloses a device for ultrasound microbubble-mediated transfection. The device comprises a liquid storage container, a floating board, an ultrasonic transducer and a culture dish, wherein the liquid storage container is used for containing an ultrasound contrast agent, the floating board is in the liquid storage container and floats in the ultrasound contrast agent, the ultrasonic transducer is installed on the floating board and suspends in the ultrasound contrast agent under the floating action of the floating board, the culture dish is suspended in the liquid storage container and is above the ultrasonic transducer. According to the device for ultrasound microbubble-mediated transfection, the ultrasonic transducer suspends in the ultrasound contrast agent through the floating board, so that the ultrasonic transducer does not contact or fix with other fixed piece; when ultrasound acts on the ultrasound contrast agent in the liquid storage container, the energy of the ultrasound is directly transmitted to the ultrasound contrast agent and hardly losses, so that the ultrasound contrast agent generates more microbubbles, so as to improve the transfection effect.

Description

technical field [0001] The invention relates to medical equipment, in particular to a device for ultrasonic microbubble-mediated transfection. Background technique [0002] With more understanding of human genes, the occurrence of many diseases is related to genes, and gene therapy can fundamentally solve the problems and become the hope for the treatment of tumors and other genetic diseases. The key to gene therapy is to increase the gene transfection rate. [0003] At present, there are two main types of tools for transferring genes: one is viral vectors, and the other is non-viral vectors, such as liposomes and plasmids. The former can transfect genes into various tissue cells, and the transfection rate is high, but the virus itself is immunogenic and can cause a specific immune response in the human body, so the safety is poor; the latter is safe, but the transfection rate is low , which is limited in clinical application. Studies have shown that using ultrasonic micr...

AI Technical Summary

Problems solved by technology

[0005] An auxiliary device for ultrasonic microbubble-mediated gene transfection is disclosed in the related art. The device is used in a laboratory to conduct ultrasonic microbubble-mediated gene transfection experiments. A plurality of scale grooves arranged up and down, a pair of adjusting iron wires, a pair of carrying iron wires and a petri dish placed on the carrying iron wires, the carrying iron wires are fixed in the large bottle, and the adjusting iron wires are located under the carrying iron wires and placed on the scale grooves, which produces The number of sonicated microbubbles is relatively small, resulting in poor transfection

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