Culture medium composition containing fermentation mother solution as well as production method and fermentation method thereof

A culture medium and composition technology, applied in the field of re-fermentation and utilization of fermented yeast liquid, can solve the problems of increasing the economic and environmental burden of enterprises, high daily operating costs, and the application of complete sets of technologies

Active Publication Date: 2018-10-19
杭州雪域生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no mature complete set of technology applied to production practice at home and abroad.
The main problem is that the one-time investment is too large, or the daily operation cost is too high, most manufacturers cannot bear it, and have to maintain the status quo of excessive emissions for a long time
[0007] With the development of the industry and the expansion of production scale, the output of fermented yeast liquid will also continue to increase. If it cannot be effectively and properly treated, it will increase the economic and environmental burden of the enterprise and hinder the healthy development of the enterprise.

Method used

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  • Culture medium composition containing fermentation mother solution as well as production method and fermentation method thereof
  • Culture medium composition containing fermentation mother solution as well as production method and fermentation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Add 0.06g of broken iron-rich yeast and 30g of corn superfine powder to 1L of Agaricus blazei mycelium liquid yeast solution, sterilize at 121°C for 30 minutes, and then cool.

[0030] The Agaricus blazei seeds were inoculated into the above-mentioned liquid medium, and liquid fermentation was carried out at a temperature of 25°C, aeration and stirring, and a fermentation time of 15 days. The cultured fermentation broth was centrifuged at 2000 rpm for processing, and the mycelium was collected, washed and dried to obtain organic enrichment. Agaricus blazei mycelium.

Embodiment 2

[0032] Add 0.18 g of broken iron-rich yeast and 10 g of corn superfine powder to 1L of Agaricus blazei mycelium liquid yeast, sterilize at 121°C for 15 minutes and then cool.

[0033] The Agaricus blazei seeds were inoculated into the above-mentioned liquid medium, and liquid fermentation was carried out at a temperature of 27°C, aeration and stirring, and a fermentation time of 3 days. The cultured fermentation broth was centrifuged at 10000 rpm, and the mycelium was collected, washed and dried to obtain organic enrichment. Agaricus blazei mycelium.

Embodiment 3

[0035] Add 0.09g of broken iron-rich yeast and 25g of corn superfine powder to 1L of Agaricus blazei mycelium liquid yeast, sterilize at 121°C for 20 minutes, and then cool.

[0036] The Agaricus blazei seeds were inoculated into the above-mentioned liquid medium, and the liquid fermentation was carried out at a temperature of 25°C, aeration and stirring, and a fermentation time of 5 days. The cultured fermentation broth was subjected to plate and frame pressure filtration with a pressure of 60 kg, and the mycelium was collected. Washing and drying to obtain organic Agaricus blazei mycelium.

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Abstract

The invention provides a culture medium composition containing a fermentation mother solution as well as a production method and a fermentation method thereof and belongs to the technical field of secondary fermentation and utilization of the fermentation mother solution. Compared with the fermentation mother solution and a mixing ratio and each component of the fermentation mother solution and afermentation raw solution, the dry weight and iron content of mycelia of agaricus subrufescens obtained by carrying out liquid fermentation through the culture medium composition containing the fermentation mother solution are remarkably improved; the culture medium composition containing the fermentation mother solution also has apparent advantages in the aspects of cost, capability of changing wastes into valuable things, environment friendliness and the like.

Description

Technical field [0001] The present invention relates to the technical field of re-fermentation and utilization of hair yeast liquid, and in particular to a culture medium composition containing hair yeast liquid and a preparation method and fermentation method thereof. Background technique [0002] With the progress of human civilization and the continuous development of the national economy, people pay more and more attention to their own health. [0003] Agaricus blazei, also known as Brazilian mushroom or Brazilian mushroom, is native to Brazil, Peru and other places and belongs to the category of edible and medicinal mushrooms. Agaricus blazei Murrill is extremely nutritious, has an excellent taste, pure and delicious taste, and has high edible value. The protein composition includes 18 kinds of amino acids, 8 kinds of essential amino acids in the human body are complete, and it also contains a variety of vitamins and ergosterol. In Japan, it is one of the treasures that the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 徐深录李肖娟
Owner 杭州雪域生物技术有限公司
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