Umbilical cord mesenchymal stem cell transportation protective liquid
A technology of MSCs and protective solution, applied in the field of transport protective solution for umbilical cord mesenchymal stem cells, can solve the problems of umbilical cord mesenchymal stem cells coagulation, decreased cell activity, etc.
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Embodiment 1
[0020] Example 1 Preparation of Umbilical Cord Mesenchymal Stem Cells
[0021] Step one umbilical cord treatment
[0022] (1) Wash the umbilical cord: transfer the umbilical cord to a 10cm sterile petri dish with sterile toothed forceps, add specimen washing solution to rinse, and repeat 2 to 3 times to remove blood stains as much as possible.
[0023] (2) Sterile tissue scissors Cut the umbilical cord into sections of about 2-3 cm, add 10-20 mL of specimen washing solution to wash the blood clot, and repeat the washing until the blood stains are basically removed, and the washing solution is clear. Eliminate blood vessels: remove two arteries and one vein of the umbilical cord according to the helical trend of blood vessels.
[0024] (3) Wash the blood stains from the umbilical cord tissue from which blood vessels have been removed with an appropriate amount of specimen washing solution, and repeat the washing until the washing solution is clear.
[0025] (4) Put the washed...
Embodiment 2
[0051] Example 2 The umbilical cord mesenchymal stem cells of the present invention are mixed with the transport protection solution
[0052]A kind of umbilical cord mesenchymal stem cell transport protection solution, which consists of 1% heparin sodium solution, 1% streptomycin sulfate solution and 96% DMEM medium aqueous solution, 1% Dazhu Rhodiola injection and 1% Composition of Xiangdan Injection.
[0053] According to the ratio in the formula, take a corresponding volume of streptomycin sulfate solution and add it to the DMEM medium aqueous solution and mix evenly. For the preparation method of umbilical cord mesenchymal stem cells, please refer to Example 1. The concentration of umbilical cord mesenchymal stem cells in the umbilical cord transportation protection solution is 5.5×10 5 ~6.5×10 5 pc / ml, aliquoted into cryovials for storage.
Embodiment 3
[0054] Example 3 The umbilical cord mesenchymal stem cells of the present invention are mixed with the transport protection solution
[0055] A kind of umbilical cord mesenchymal stem cell transport protection solution, which consists of 2% heparin sodium solution, 2% streptomycin sulfate solution and 92% DMEM medium aqueous solution, 2% Dazhu Rhodiola injection and 2% Composition of Xiangdan Injection.
[0056] According to the ratio in the formula, take a corresponding volume of streptomycin sulfate solution and add it to the DMEM medium aqueous solution and mix evenly. For the preparation method of umbilical cord mesenchymal stem cells, please refer to Example 1. The concentration of umbilical cord mesenchymal stem cells in the transport protection solution is 5.5×10 5 ~6.5×10 5 pc / ml, aliquoted into cryovials for storage.
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