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Umbilical cord mesenchymal stem cell transportation protective liquid

A technology of MSCs and protective solution, applied in the field of transport protective solution for umbilical cord mesenchymal stem cells, can solve the problems of umbilical cord mesenchymal stem cells coagulation, decreased cell activity, etc.

Inactive Publication Date: 2018-11-23
QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the invention is to solve the problem of coagulation and decreased cell activity of umbilical cord mesenchymal stem cells during transportation, and provides a protective solution for umbilical cord mesenchymal stem cell transportation, which has a strong anticoagulant effect and can effectively protect umbilical cord mesenchymal stem cells. Cell activity of stem cells during transport

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Preparation of Umbilical Cord Mesenchymal Stem Cells

[0021] Step one umbilical cord treatment

[0022] (1) Wash the umbilical cord: transfer the umbilical cord to a 10cm sterile petri dish with sterile toothed forceps, add specimen washing solution to rinse, and repeat 2 to 3 times to remove blood stains as much as possible.

[0023] (2) Sterile tissue scissors Cut the umbilical cord into sections of about 2-3 cm, add 10-20 mL of specimen washing solution to wash the blood clot, and repeat the washing until the blood stains are basically removed, and the washing solution is clear. Eliminate blood vessels: remove two arteries and one vein of the umbilical cord according to the helical trend of blood vessels.

[0024] (3) Wash the blood stains from the umbilical cord tissue from which blood vessels have been removed with an appropriate amount of specimen washing solution, and repeat the washing until the washing solution is clear.

[0025] (4) Put the washed...

Embodiment 2

[0051] Example 2 The umbilical cord mesenchymal stem cells of the present invention are mixed with the transport protection solution

[0052]A kind of umbilical cord mesenchymal stem cell transport protection solution, which consists of 1% heparin sodium solution, 1% streptomycin sulfate solution and 96% DMEM medium aqueous solution, 1% Dazhu Rhodiola injection and 1% Composition of Xiangdan Injection.

[0053] According to the ratio in the formula, take a corresponding volume of streptomycin sulfate solution and add it to the DMEM medium aqueous solution and mix evenly. For the preparation method of umbilical cord mesenchymal stem cells, please refer to Example 1. The concentration of umbilical cord mesenchymal stem cells in the umbilical cord transportation protection solution is 5.5×10 5 ~6.5×10 5 pc / ml, aliquoted into cryovials for storage.

Embodiment 3

[0054] Example 3 The umbilical cord mesenchymal stem cells of the present invention are mixed with the transport protection solution

[0055] A kind of umbilical cord mesenchymal stem cell transport protection solution, which consists of 2% heparin sodium solution, 2% streptomycin sulfate solution and 92% DMEM medium aqueous solution, 2% Dazhu Rhodiola injection and 2% Composition of Xiangdan Injection.

[0056] According to the ratio in the formula, take a corresponding volume of streptomycin sulfate solution and add it to the DMEM medium aqueous solution and mix evenly. For the preparation method of umbilical cord mesenchymal stem cells, please refer to Example 1. The concentration of umbilical cord mesenchymal stem cells in the transport protection solution is 5.5×10 5 ~6.5×10 5 pc / ml, aliquoted into cryovials for storage.

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Abstract

The invention discloses umbilical cord mesenchymal stem cell transportation protective liquid which is prepared from ingredients in volume percent: 1% to 3% of heparin sodium solution, 1% to 3% of streptomycin sulfate solution, 80% to 96% of DMEM culture medium water solution, 1% to 2% of sofren injection and 1% to 2% of xiangdan injection. According to the umbilical cord mesenchymal stem cell transportation protective liquid disclosed by the invention, different ingredients of transportation protective liquid is utilized to perform storage experiments and anticoagulation experiments on umbilical cord mesenchymal stem cells having the same source; after being treated for the same time, statistics are performed on survival rates of all the treated umbilical cord mesenchymal stem cells; results show that the umbilical cord mesenchymal stem cell transportation protective liquid disclosed by the invention can ensure a higher cell survival rate; the umbilical cord mesenchymal stem cell transportation protective liquid is prepared from the sofren injection and the xiangdan injection, so that an anticoagulation effect of stem cells is ensured in compatible use, the heparin sodium use amount is simultaneously reduced, side effects caused by the heparin sodium is reduced, and safety of the umbilical cord mesenchymal stem cell transportation protective liquid disclosed by the invention is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a transport protection solution for umbilical cord mesenchymal stem cells Background technique [0002] Stem cells are a type of pluripotent cells with self-replicating ability. Under certain conditions, it can differentiate into a variety of functional cells. Stem cells are divided into embryonic stem cells (Embryonic stem cells, ES cells) and adult stem cells (Somatic stem cells) according to their developmental stages. Stem cells are divided into three categories according to their developmental potential: totipotent stem cells (TSCs), pluripotent stem cells (Pluripotent stem cells) and unipotent stem cells (Unipotent stem cells). Stem cells are not fully differentiated and immature cells, which have the potential to regenerate various tissues and organs and the human body, and are called "universal cells" in the medical field. [0003] In recent years, the upsurge of preserving ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021
Inventor 戚凤春宫丽张炳强章宏金铎于淼于露
Owner QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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